Labshake search
Citations for GE Life Sciences :
51 - 100 of 710 citations for 7 HYDROXY 4' METHOXYFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
-
bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
-
bioRxiv - Cell Biology 2021Quote: ... and mCherry (5% laser power, 0,05 exposure) were recorded at 7 minutes intervals and data were analyzed using SoftWoRx (GE Healthcare). The time from nuclear envelope breakdown (NEBD ...
-
bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
-
bioRxiv - Immunology 2021Quote: ... supernatant was adjusted to pH=7 and loaded onto a High-Trap Prot G column (GE Healthcare, Chicago, Illinois, USA) using an Äkta system (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
-
bioRxiv - Biochemistry 2019Quote: ... Following dialysis the E215C K490 construct was reacted for 4 h at 4°C with Cy3-maleimide (GE Healthcare, PA13131) at a motor/Cy3 dye ratio of 1:10 as previously described (Tomishige et al ...
-
bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
-
bioRxiv - Molecular Biology 2019Quote: ... and glutathione Sepharose 4 Fast Flow (GE Healthcare), respectively ...
-
bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
-
bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
-
bioRxiv - Molecular Biology 2019Quote: ... The reaction products were electrophoretically separated on a denaturing 24% polyacrylamide/7 M urea gel and visualized with a phosphorimager (Typhoon 9410; GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
-
bioRxiv - Biochemistry 2021Quote: ... 0.125 pmol of FAM-labeled RNA product were separated by gel electrophoresis (20 % polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... The reaction products were resolved on 10-14 % polyacrylamide gels containing 7 M urea and analyzed with a Typhoon TRIO Phosphorimager (GE Healthcare).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
-
bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
-
bioRxiv - Pathology 2019Quote: ... using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France). Bi-dimensional (B ...
-
bioRxiv - Pathology 2019Quote: ... Electrophoresis was performed at 100V (200 mA) for 1 h and 150V (300 mA) for 7-8 hrs in Ettan DALT six apparatus (GE Healthcare). After electrophoresis ...
-
bioRxiv - Biochemistry 2019Quote: For conjugation of pG to a SMCC-functionalized ODN an aliquot of pG was buffer exchanged to (100 mM Sodium Phosphate, 25 µM TCEP, pH 7) using a PD10 desalting column (GE Healthcare). Subsequently ...
-
bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
-
bioRxiv - Biophysics 2021Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
-
bioRxiv - Cell Biology 2021Quote: ... 3D widefield datasets were acquired using a 100x 1.4NA Uplan S Apochromat objective on a DeltaVision 2 Ultra microscope equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare), computationally deconvolved using the enhanced ratio constrained iterative deconvolution algorithm ...
-
bioRxiv - Microbiology 2020Quote: ... Germany) and differentiated into macrophages by culture for 7 days in RPMI1640 medium supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences ...
-
bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
-
bioRxiv - Microbiology 2022Quote: ... and analysed by denaturing 7% polyacrylamide gel electrophoresis and by phosphorimaging with a Typhoon FLA-9500 instrument and ImageQuant TL software (GE Healthcare). Potential Cu2+ scavenging-by-buffer effects (Mash et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
-
bioRxiv - Biochemistry 2023Quote: ... Purification of His-tagged domains was achieved by nickel-affinity using purification buffer supplemented with 1 mM DTT and gel filtration chromatography in 50mM ammonium bicarbonate pH 7 using a Superdex 200 Increase 10/300 column (GE Healthcare) following published protocols67 ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl pH 7) and size exclusion chromatography was performed using HiLoad 16/600 Superdex 75 pg Column (GE28-9893-33, GE Healthcare) attached to BioLogic DuoFlow Chromatography system (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: ... and 5 μg of total RNA was separated by 15% (wt/vol) acrylamide/7 M urea gel and subsequently transferred to a Hybond-NX nylon membrane (GE healthcare) after gel electrophoresis ...
-
bioRxiv - Plant Biology 2024Quote: ... gel containing 7 M urea and buffered with 0.5 X TBE using an SE600 standard dual-cooled gel electrophoresis unit (GE Healthcare, USA) until the bromophenol blue dye reached the bottom of the gel ...
-
bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
-
bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
-
bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
-
bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
-
bioRxiv - Molecular Biology 2021Quote: ... nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used ...
-
bioRxiv - Biochemistry 2022Quote: ... and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare), respectively ...
-
bioRxiv - Biochemistry 2021Quote: ... or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) following the instructions provided by the manufacturer with appropriate modifications ...
-
bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...