Labshake search
Citations for GE Life Sciences :
101 - 150 of 171 citations for 7 CHLOROHEPTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleic acid (400 ng) was spotted on to Amersham Hybond N+ nylon membrane (GE Healthcare Lifesciences), air-dried and UV cross-linked for 3 min using a transilluminator ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.1% (v/v) trifluoroacetic acid and applied to a Superdex 30 3.2/300 column (GE Healthcare). Fractions containing crosslinked peptides were evaporated to dryness ...
-
bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
-
bioRxiv - Microbiology 2021Quote: ... and NAD(P)H were extracted from approximately 8 ml of OD750 nm = 0.7-1.0 cultures sampled onto 47 mm diameter glass microfiber filters of a 1.2 μm pore size (GE Healthcare, Little Chalfont, England), followed by immediate shock freezing ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: Transthoracic echocardiography was performed using a 10-14-MHz i13 probe and the Vivid 7 Ultrasound machine (GE Healthcare Ultrasound, Horten, Norway). Mice were lightly anesthetized with isoflurane ...
-
bioRxiv - Biochemistry 2023Quote: ... The synthesized DNA products were separated on denaturing 30% PAGE with 7 M urea in 1xTBE and visualized on a Typhoon 9400 (GE Healthcare, USA).
-
bioRxiv - Cancer Biology 2019Quote: ... The fluorophore conjugated CMP-sialic acids were then purified on a HiTrap® Q HP (GE Healthcare) column and eluted with a 0-100% gradient of NaCl elution buffer (150 NaCl ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
-
bioRxiv - Cell Biology 2022Quote: ... URA3: CaUra3pXhoI and CaURA3m81) and Amersham ECL Direct Nucleic Acid Labelling and Detection System kit (GE Healthcare UK Limited ...
-
bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
-
bioRxiv - Plant Biology 2020Quote: The enzymes’ buffer was changed to 10 mM MOPS (pH 7) using the PD Spintrap™ G-25 (GE Healthcare, 28-9180-04) protocol ...
-
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden) (75) ...
-
bioRxiv - Physiology 2020Quote: ... transthoracic echocardiography was performed for cardiac examination using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France) as described in the supplemental methods herein ...
-
bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
-
bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
-
bioRxiv - Physiology 2020Quote: ... Examinations were carried out with a linear array i13L probe (10-14 MHz) using a Vivid 7 Dimension ultrasound system (GE Healthcare Ultrasound, Horten, Norway). The operator was blinded to the genotype of the mice ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
-
bioRxiv - Immunology 2020Quote: ... acid-eluted immunoprecipitates were separated by SDS-PAGE under reducing conditions and transferred to HybondTM ECLTM membranes (GE Healthcare). Membranes were incubated sequentially with the different primary antibodies and developed with the appropriate HRP-conjugated secondary antibody using enhanced chemiluminescence (ECL) ...
-
bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
-
bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
-
bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...
-
bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
-
bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
-
bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
-
bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
-
bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
-
bioRxiv - Bioengineering 2019Quote: ... The nucleic acids were visualized using a G:BOX Chemi imager (Syngene, USA) and quantitative analyzed by ImageQuant (GE Healthcare, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
-
bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
-
bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
-
bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
-
bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
-
bioRxiv - Molecular Biology 2023Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
-
bioRxiv - Biochemistry 2020Quote: ... PDI (in PBS, pH 7) was submitted to size exclusion chromatography on a Superose™ 12 10/300 GL column (GE Healthcare, Cat. No. 17-5173-01) at a flow rate of 0.5 ml/min with PDI eluting between 11 and 14 ml.
-
bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
-
Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
-
bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
-
bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
-
bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
-
bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... All recombinant proteins were purified using Ni2+-nitrilotriacetic acid agarose (Ni-NTA) column followed by size-exclusion chromatography (Superdex 200 column from GE Healthcare) in a final buffer containing 50 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).