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Citations for GE Life Sciences :
551 - 600 of 4411 citations for 7 Bromo 3 4 dihydro 1H benzo e 1 4 diazepine 2 5 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Pathology 2019Quote: ... Electrophoresis was performed at 100V (200 mA) for 1 h and 150V (300 mA) for 7-8 hrs in Ettan DALT six apparatus (GE Healthcare). After electrophoresis ...
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bioRxiv - Molecular Biology 2019Quote: ... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate made by sonication was centrifuged at 11,000 rpm for 30 min at 4◦C and the clear supernatant was purified through Ni-NTA column chromatography (GE Healthcare) following kit protocols ...
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bioRxiv - Genetics 2021Quote: ... 2D gels were exposed for 4 hours in a phosphor screen cassette and the signal was detected with a Typhoon Trio phosphoimager (GE healthcare).
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by centrifugation at 40,000 rpm in a Type 45 Ti rotor for 60 minutes at 4 °C and applied onto a pre-equilibrated HisTrap HP column (GE Healthcare) in CENP-E lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1ko blot) Bis-Tris gels (Thermo) before transferring to PDVF membranes (GE Healthcare). Membranes were blocked in 5% milk and blotted with primary and secondary HRP antibodies ...
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bioRxiv - Biophysics 2019Quote: Proteins were concentrated with appropriate centrifugal filtration devices (Amicon) and further purified by SEC at 4°C using an ÄKTAFPLC chromatography system (GE Healthcare). For crystallographic studies ...
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bioRxiv - Microbiology 2019Quote: ... Images were acquired with 200 ms exposure time every 4 minutes for at least 6 h at 37°C using Softworx (Applied Precision) software ...
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bioRxiv - Biochemistry 2019Quote: ... of total cellular protein was separated by SDS-PAGE using 4-12.5 % Bis-Tris gels and transferred to nitrocellulose membrane (GE Healthcare # 10600006). SeeBlue pre-stained protein standard (Invitrogen # LC5925 ...
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bioRxiv - Biochemistry 2019Quote: Peak fractions from the IEX were concentrated to < 4 ml and loaded onto a Superdex 16/60 gel filtration column (GE Healthcare) in Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... The affinity-purified protein was TEV-cleaved o/n at 4°C and subsequently subjected to gel filtration on an equilibrated Superdex 75 gel filtration column 16/600 (GE Healthcare). Monomer fractions were pooled and concentrated to 1 mg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... was resolved using 4–15% Mini-PROTEAN® TGX™ gels and transferred to Hybond-P 0.45µm PVDF membrane (GE Healthcare). Membranes were blocked in Roche block and incubated with primary antibodies over night or for 3 hours at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by the addition of PreScission Protease (~100 μL at 76 mg/mL) (GE Healthcare) and the eluted ATAD2B bromodomain protein was concentrated to a total volume of approximately 3 mL ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was then mixed with GFP resin which was produced by crosslinking nanobody71 to NHS-Activated Sepharose 4 Fast Flow beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The purification steps for both POLA1–POLA2–PRIM1–PRIM2 and p50–Ctc1–Stn1–Ten1 were performed at 4°C using an AKTA chromatography system with prepacked columns (GE Healthcare), following the same protocol ...
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bioRxiv - Biophysics 2020Quote: ... and non-deamidated (pure N373 wildtype) GII.4 P dimer species was achieved by cation exchange chromatography using a 6 ml Resource S column (GE Healthcare) at 6 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation with primary antibodies overnight at 4°C followed by the addition of Protein A or G Sepharose beads (GE Healthcare) for 2-3 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Biochemistry 2022Quote: All chromatography purification steps were carried out at 4°C using an ÄKTA pure fast protein liquid chromatography (FPLC) system (GE Healthcare). All buffer solutions were chilled prior to use ...
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bioRxiv - Microbiology 2022Quote: ... The infusions were stored over-night at 4°C before the leaves were removed and the aqueous solutions were sterile-filtered (200 μM filter, Whatman/GE Healthcare). Afterwards ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples were run on 5∼20% precast gradient SDS-PAGE (ATTO) or 4∼20% precast gradient gel (Shanghai WSHT Biotechnology Inc.) and then transferred to Hybond ECL membranes (GE Healthcare) followed by western blot analysis with the indicated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The combined sample was incubated for an additional 30 min at 4 °C then injected onto a Superdex 200 column (GE Healthcare) equilibrated in 25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Lysate equivalent of approximately 2×105 cells per well were run on NuPage 4-12% Bis-Tris Gel (Novex) and transferred to Amersham Hybond-P PVDF membranes (GE Healthcare). Membranes were blocked with 2% nonfat dry milk (Lab Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 µL of supernatants were stored as input and the rest were incubated with the lysis buffer-prewashed G-sepharose beads (50 µL of 50% slurry per each reaction; Protein G-Sepharose 4 Fast Flow, GE Healthcare) for 3 h at 4 °C while rotating ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: ... SMCHD1 or γH2AX) and 30µL of preblocked Protein G Sepharose 4 Fast Flow 50% bead slurry (GE Healthcare, #17-0618-01) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... ~300 μL of 5 μM complex were mixed at a 1:1 molar ratio and incubated for 30 minutes at 4° before injection onto a Superdex200 10/300 GL analytical scale column (GE Healthcare). For CRY:PER2 CBD complexes used for BLI or CRY1:PER2 CBD:CLOCK:BMAL1 bHLH-PAS:Ebox DNA for cryo-EM ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE Healthcare Biosciences, 30 min, 4°C, 2,100 r.p.m.). PBMCs were extensively washed in cold phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated protein was spun down 15 min in a bench-top centrifuge (4°C) and immediately loaded onto a HiLoad 16/600 Superdex 200 column (GE Healthcare), preequilibrated in gel filtration buffer consisting of 20 mM HEPES pH 7.5 ...