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Citations for GE Life Sciences :
651 - 700 of 2217 citations for 7 ACETOXY 2 CHLORO 1 HEPTENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
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bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Cell Biology 2023Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated with HRP-conjugated secondary anitbodies at 1:3,000 for 1 h at room temperature and bands developed using ECL Prime Western Blotting Detection Reagent (GE Healthcare). Blots were subsequently incubated with 1:20,000 HRP-conjugated β-actin (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... For further purification the protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted 1:1 with 20 mM HEPES KOH (pH 7.5) and loaded on a HiTrap Heparin HP (GE Healthcare) column and washed with Buffer A (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell culture media was replaced with a 1:1 mixture of 1X HBSS (SH30588.02, GE Healthcare Life Sciences, United States) with 20 mM HEPES (BP310-1 ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... 50-250 μl of plasma were used for immunoglobulin purification via 1:1 mixture of proteinA/proteinG resin (GE Healthcare). Slurry was washed once with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... 76 mm × 24 mm super frost glass slides were coated with a solution made of 14:1:1 ethanol:acetic-acid:PlusONE Bind-Silene (GE Healthcare; vol:vol:vol). In parallel ...
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bioRxiv - Neuroscience 2020Quote: ... 1:20,000-1:40,000),immunoreactive bands on the membranes were detected by enhanced chemiluminescence (ECL Plus; GE Healthcare Bio-Sciences). For total protein expression ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity and kinetic constants were determined by fitting the corrected sensorgrams with the 1:1 Langmuir model using BIAevaluation v3.0 software (GE Healthcare). The equilibrium binding constant KD was determined by the ratio of the binding rate constants kd/ka.
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bioRxiv - Biophysics 2021Quote: ... 25 µM of purified SpyCatcher-GFP was mixed with excess purified SpyTag-SAT09 (1:1.5) and incubated at 4°C for 1 hr and loaded on a HiPrep Desalting Column (GE Healthcare) equilibrated with 10mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Plant Biology 2019Quote: ... They were determined by fitting the experimental data with the 1:1 Langmuir model (BIA-evaluation software 4.1, GE Healthcare). The binding constants were calculated by two methods ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
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bioRxiv - Cell Biology 2020Quote: ... 100 µL of CENP-E2055-2608 and CENP-E2055- 2358 at 1 mg·ml-1 were run on a calibrated Superdex-200 10/300 GL Increase (GE Healthcare) size exclusion column pre-equilibrated in gel filtration buffer (described above ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and the NiRAN construct (nsp12 amino acid residues 1-259) were cloned into pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST tag followed by a PreScission protease site ...
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... was mixed with fibrinogen solution in a 7.5:1 molar ratio for 1 hour at room temperature and then filtered through a HiTrap desalting column (GE Healthcare) packed with Sephadex G-25 resin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA and 1 mM DTT) and loaded onto a Mono Q 10/100 GL column (GE Healthcare, Inc.) equilibrated in buffer E and eluted with a 160 mL linear gradient of 0.3–0.5 M NaCl in buffer E ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Cell Biology 2020Quote: ... using 1 ml Resource S column (GE healthcare) connected to a nano-HPLC (Ultimate 3000 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-anti rabbit secondary (GE Healthcare; 1:5000) and HRP-anti mouse secondary (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... donkey anti-rabbit (NA934, GE Healthcare, 1:10000) and sheep anti-mouse (NA931 ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse secondary antibody (1:4000, GE Healthcare) or alpaca anti-chicken (1:4000 ...
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bioRxiv - Microbiology 2019Quote: ... using 1 ml HisTrap HP columns (GE Healthcare) followed by size exclusion chromatography (HiLoad 16/60 Superdex 200 ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Bovine Serum Albumin (BSA, GE Life Sciences) and 0.03% Triton X-100 in 0.1 M PBS before overnight incubation with primary antibodies (rabbit anti-cldn5 ...
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bioRxiv - Cell Biology 2021Quote: ... and donkey anti-rabbit (GE healthcare; 1:5000).
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bioRxiv - Molecular Biology 2020Quote: ... anti-mouse (1:10,000, GE Healthcare, Cat. #NA931V) anti-Rat (1 ...