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Citations for GE Life Sciences :
351 - 400 of 788 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-fused IQGAP1-N and IQGAP1-C were purified using Glutathione Sepharose 4B (17-0756-01; GE Healthcare). Next ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... These were stored in a chilled compartment at 9°C within a skirted 384 well plate (GE Healthcare) before being washed over the chip surface in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
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bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: All SPR measurements were performed at 25° C using a Biacore 3000 instrument (BIAcore, GE Healthcare, Piscataway, NJ) as described previous24,25 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Immunology 2022Quote: The hcAb-binding properties of B10 were analyzed at 25 °C on a Biacore T200 instrument (GE Healthcare) using 10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then separated in 10% SDS polyacrylamide gels and transferred to Hybond C nitrocellulose membranes (GE Healthcare). After blocking with 5% non-fat milk in PBS/0.05% Tween ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Immunology 2021Quote: All surface plasmon resonance experiments were performed at 25°C using a Biacore T200 instrument (Cytiva, GE Healthcare) equipped with a flat carboxymethyldextran sensor chip (CMDP ...
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bioRxiv - Cell Biology 2021Quote: ... The experiments were performed at 25°C using a BiacoreTM T200 instrument (GE Healthcare Life Sciences, Uppsala, Sweden) flowed with EP+ buffer (10mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare). Proteins were exchanged into 150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
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bioRxiv - Biochemistry 2022Quote: Ca2+ binding was characterized by ITC at 25 °C using a VP-ITC microcalorimeter (GE Healthcare, Northampton, MA) with a cell volume of 1.4619 ml in Na+ or K+ containing buffers (20 mM Tris pH 7.9 ...
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bioRxiv - Microbiology 2023Quote: The surface plasmon resonance (SPR) experiments were performed at 25 °C on a BIAcore 2000 instrument (GE Healthcare). DsrC-trisulfide was immobilized on a NTA sensor chip (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
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bioRxiv - Cell Biology 2024Quote: ... Live imaging was carried out in a chamber at 37°C using a Deltavision Core (Applied Precision™) microscope equipped with a CoolSnap HQ2 (Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... Time-lapse imaging was carried out at 27 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision) comprising an Olympus IX70 widefield inverted epifluorescence microscope ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Genomics 2019Quote: ... hybridization (at 68°C in 50% formamide) and washing was done following the instructions of the manufacturer (GE Healthcare). The riboprobes were obtained by rub-off transcriptions of linearized pBlueScript plasmids containing the full retrozymes in the presence of DIG-UTP (Roche Diagnostic GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... dried for one hour at 80°C and exposed overnight to Phosphor screen in a cassette (GE healthcare, USA). Images were captured in Fujifilm FLA 5100 scanner (Japan ...
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bioRxiv - Biochemistry 2020Quote: ... Analytical size exclusion chromatography was performed at 24 °C using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a mobile phase containing 10 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Cell Biology 2022Quote: Living embryos were imaged at 21°C using a DeltaVision OMX V3 Blaze microscope (GE Healthcare Life Sciences, UK). The system was equipped with a 60x/1.42 NA oil UPlanSApo objective (Olympus Corp.) ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...