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Citations for GE Life Sciences :
651 - 700 of 3958 citations for 7 Chloromethyl 5H 1 3 thiazolo 3 2 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... at 4 °C overnight and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Epidemiology 2019Quote: ... measles MV-N was purified by chromatography on a MonoQ HR 5/5 strong anion exchange column (GE Healthcare, Piscataway, NJ) prior to use ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and then separated sequentially on a 5-ml HiTrap Q HP column followed by a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: nFhGST was purified by affinity chromatography from a Fasciola hepatica adult fluke extract using a 5/5 GSTrap HP column (GE Healthcare) following a pre-established protocol9 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Plant Biology 2020Quote: The enzymes’ buffer was changed to 10 mM MOPS (pH 7) using the PD Spintrap™ G-25 (GE Healthcare, 28-9180-04) protocol ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden) (75) ...
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bioRxiv - Physiology 2020Quote: ... transthoracic echocardiography was performed for cardiac examination using a Vivid 7 dimension cardiovascular ultrasound system equipped with a probe of 9-14 MHz frequency (GE Healthcare, Vélizy, France) as described in the supplemental methods herein ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... The membrane was then incubated for one hour at room temperature in presence of a sheep anti-mouse whole IgG HRP (GE Healthcare) secondary antibody diluted 1/5000 in blocking buffer ...
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bioRxiv - Plant Biology 2021Quote: ... linked to horseradish peroxidase was diluted 1:10000 in TBS and used for one hour at RT before detection by chemiluminescence using AmershamTM ECLTM Prime Western Blotting Detection Reagent (GE Healthcare) following manufactures instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... All capture surfaces were regenerated with one 30-s pulse of 10 mM glycine–HCl (pH 1.5, (GE Healthcare, Marlborough, MA). Kinetic parameters were obtained by globally fitting the data to a 1:1 binding model with mass transport limitation using Scrubber 2.0c Evaluation Software ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted from rosette leaves of one individual plant using the Nucleon Phytopure genomic DNA extraction kit (GE Healthcare), and whole genome bisulfite sequencing (WGBS ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: ... After two additional washing steps with TBS-T and one with TBS the blots were developed by incubation with Amersham ECL Western Blotting Detection Reagents (GE Healthcare) for 2 min and detected on an ImageQuant (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The medium was changed one day after transfection and live cells were imaged on an IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification before the plate was returned to the incubator and cells were allowed to expand ...
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bioRxiv - Cell Biology 2019Quote: ... 2) A Deltavision Elite System (GE Healthcare) equipped with an IX-71 inverted microscope (Olympus) ...
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bioRxiv - Immunology 2019Quote: ... 20U/ml Xuri IL-2 (GE Healthcare) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Biochemistry 2021Quote: ... DrrA-OCRL or DrrA-INPP5E were then loaded onto a 1 mL MonoS (5/50 GL) cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM HEPES pH 7 ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged using Typhoon 5 Imager (GE Healthcare) to record signals from the Cy5 dye ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare) was used for further purification which resulted in a monodisperse and clear INO80 complex ...
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bioRxiv - Biophysics 2019Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and finally eluted with 220 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... on a MonoQ 5/50 GL column (GE Healthcare). Sample purification and analysis with HPLC were performed using Agilent 1260 Infinity (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl of streptavidin beads (GE Healthcare, Chicago, USA) per O.D.254 unit was added ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... A HisTrap HP column (5 ml, GE Healthcare, France), prepacked with Ni High Performance Sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were scanned using a Typhoon 5 (GE Healthcare) and the signal intensity quantified using Image Studio Lite (LI-COR Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... applied to a 5 ml HisTrap HP (GE Healthcare), and washed with lysis buffer using an Äkta pure fast-performance liquid chromatography system (GE Healthcare,) ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare, Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... using a 5 mL HisTrap FF column (GE Healthcare). Bacterial pellets were lysed in a buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... using a SD200 Increase 5/150 column (GE Healthcare) at 0.4 ml/min with 1 frame recorded per second (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... first with HisTrap HP 5 mL columns (GE Healthcare) and next with a size-exclusion HiLoad Superdex 200 PG column (GE healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting sample was applied to a 5 ml HiTrap HP heparin column (GE Healthcare) equilibrated with TGE containing 50 mM NaCl and 1 mM β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and eluted with imidazole ...