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Citations for GE Life Sciences :
251 - 300 of 2417 citations for 6H Thieno 2 3 b pyrrole 5 carboxylicacid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and 1% (v/v) ampholytes (pH 3-11 NL; GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2020Quote: ... cDNA constructs were inserted into the pGEX6P-3 plasmid (GE Healthcare) and expressed as GST-fusion proteins in High efficiency BL21(DE3 ...
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bioRxiv - Immunology 2021Quote: ... 3 or 4 of a Series S CM5 chip (GE Healthcare) functionalised with an anti-His capture kit (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: Early passage HUVECs were coated on Cytodex-3 beads (GE Healthcare) at a density of 10 million cells/40 μl beads and incubated in suspension for 3-4 hours with gentle mixing every hour ...
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Conjugation of inhibitors to beads was performed by carbodiimide coupling to ECH Sepharose 4B (CTx-0294885, VI16832 and PP58) or EAH Sepharose 4B (purvalanol B) (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... T cells were depleted by erythrocyte rosetting using sheep red blood cells and B cells were separated by Ficoll density gradient centrifugation as recommended by the manufacturer (GE Healthcare). The remaining erythrocytes were lysed in red blood cell lysis buffer (155 mM NH4Cl ...
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bioRxiv - Biochemistry 2021Quote: ... The RBD/RA of B-RAF (residues 150 -233) and RASSF5 (residues 199 - 367) were subcloned into pGEX-4T2 (GE Healthcare) to produce N-terminal GST fusion proteins ...
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Microbiology 2023Quote: ... Peak fractions were collected and diluted with buffer B to a conductivity of 18 mS/cm and loaded onto HiTrap SP columns (GE healthcare). A linear gradient of buffer B containing 1 M NaCl was used for elution ...
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bioRxiv - Microbiology 2023Quote: ... The sample was additionally diluted with buffer B to a conductivity of 18 mS/cm and loaded onto a Heparin column (GE healthcare). The protein was eluted with a linear gradient of buffer B containing 1 M NaCl ...
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bioRxiv - Biophysics 2023Quote: ... we repeated the dilution in Buffer B to 18 mS/cm conductivity and loaded it onto HiTrap SP columns (GE Healthcare). For elution ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-column digestion and the eluate was further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... The protein solubilized in the previous step were subjected to a Ni HP-5 column (5 mL, GE Healthcare, USA), previously equilibrated with 5 CV of Buffer C (buffer C (50 mM sodium phosphate pH 7.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Cell Biology 2019Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C with shaking at 100 rpm ...
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bioRxiv - Immunology 2022Quote: ... a Histrap excel 5 mL column (GE Healthcare) was used to isolate proteins ...
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bioRxiv - Plant Biology 2019Quote: ... pH 7.9 through NAP-5 column (GE Healthcare) and brought to the desired concentration either by dilution or by concentration through Amicon-Ultra device (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... a HisTrap HP 5 ml column (GE Healthcare) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... a MonoQ 5/50 GL column (GE Healthcare) was required to completely remove the GST-tag ...
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bioRxiv - Biochemistry 2020Quote: ... using a 5 mL HisTrap column (GE Healthcare). The bound protein was eluted using a linear imidazole gradient (0 - 0.5 M ...
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bioRxiv - Biophysics 2021Quote: ... while a Tricorn 5/20 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... a 5 ml HisTrap column (GE Healthcare, UK) was used for immobilized metal affinity chromatography (IMAC) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented 5% horse serum (GE Healthcare Life Sciences), 1% penicillin/streptomycin (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... using a 5 mL StrepTrap HP (GE Healthcare). The washing buffer contained 100 mM Tris pH 8.0 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 5 ml HisTrap™ HP column (GE Healthcare). For further purification steps the column was connected to a liquid chromatography system (Azura ...
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bioRxiv - Molecular Biology 2020Quote: ... blocked with 5% membrane blocking agent (GE Healthcare) in TBS and incubated with the corresponding primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC Column: 5 ml HisTrap HP (GE Healthcare); Gel Filtration Column ...
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bioRxiv - Molecular Biology 2022Quote: ... loaded onto 5 ml HisTrap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Microbiology 2019Quote: ... or 5 ml GSTrap HP column (GE Healthcare), depending on the fused tag ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% fetal bovine serum (FBS; Hyclone/GE Healthcare) in DMEM/F12 (Thermo Fisher Scientific)] for 45 min at 37°C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... A 5 ml HiTrap desalting column (GE Healthcare) was used to exchange the complex buffer into 100 mM ammonium acetate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... supplemented with 5% fetal bovine serum (GE Healthcare) and 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
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bioRxiv - Microbiology 2020Quote: ... 300 ml NaCl and eluted via a gradient of increasing imidazole (0-500 mM, Buffer B) using a ÄKTAPurifier™ System (GE Healthcare). After this first purification ...
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bioRxiv - Neuroscience 2021Quote: ... After washing again for 5 x 5 min western blots were visualized with ECL plus Western Blotting detection system (#RPN2231, GE Healthcare) or Immobilon Forte Western HRP substrate (#WBLUF ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Microbiology 2019Quote: ... and was chromatographed sequentially on a 5-ml HiTrap Q HP column and on a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...