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Citations for GE Life Sciences :
201 - 250 of 3692 citations for 6H Pyrazino 1 2 c pyrimidin 6 one octahydro 2 7 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
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bioRxiv - Biochemistry 2021Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4°C by injecting sample onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Microbiology 2021Quote: ... and applied to column with 2 ml of Ni2+-NTA-agarose (GE Healthcare) equilibrated with wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was loaded on a 2×5ml HisTrap HP column (GE Healthcare) pre-equilibrated with HisBind A ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT on a HiLoad Superdex 75 26/60 column (GE Healthcare). After treatment with Prescission protease overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked in 2% ECL Prime™ Blocking Agent (GE Healthcare, RPN418) in TBST (Tris-Buffered Saline (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... before incubation 2 h with 500 μL CNBr-activated Sepharose beads (GE Healthcare) coupled to monoclonal Rho-1D4 antibody (University of British Columbia ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Fractions were collected in a Whatman 2 ml 96-well plate (GE Healthcare) using a horizontal snaking pattern and were concatenated into 24 final fractions for proteomic analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 mM TCEP) by using a Superdex S75 16/60 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated IgG F(ab)2 donkey anti-rabbit (GE Healthcare), and HRP-conjugated affinity-purified goat anti-mouse IgG (Jackson).
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bioRxiv - Systems Biology 2020Quote: ... and filtered through a 10 kDa MWCO spin column (Vivaspin 2; GE Healthcare) and quantified by BCA assay (Pierce).
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bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
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bioRxiv - Systems Biology 2021Quote: ... agarose-GSH resin and Ni+2-NTA resin were from GE Healthcare (USA); and restriction enzymes from Thermo Fisher (USA) ...
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bioRxiv - Immunology 2020Quote: ... and supplemented to determine culture optima using [200U/mL] IL-2 (GE Healthcare), [155U/mL] IL-7 (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM MgCl2) using the PD-10 desalting columns (GE Healthcare, Piscataway, NJ). Quantification of VP2 particles was determined by absorbance at A280 with NanoDrop (NanoDrop2000 ...
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bioRxiv - Biochemistry 2022Quote: MCherry-kindlin 2 was bound on a HisTrap FF crude column (GE Healthcare) and eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... another 2 ml of buffer and 10 μl of PreScission protease (GE Healthcare) were added to the column followed by overnight incubation to cleave off the AVR proteins from the GST resin ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare; Chicago ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of carboxylate-modified paramagnetic beads (Sera-Mag Speed Beads, GE Healthcare), 0.5 μg solids/μl in water was added ...
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bioRxiv - Microbiology 2023Quote: ... 10 mM NaCl and 2 mM DTT by a desalting column (GE Healthcare), and was further purified by ion exchange chromatography with Resource Q column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was subsequently incubated with 2 ml Ni-NTA (GE Healthcare) per 1,000 ODs for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Zoology 2021Quote: ... One of the obtained PCR products was purified with ExoProStar 1-Step kit (GE Healthcare US77702) and Sanger-sequenced directly at Macrogen (Netherlands) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of purified sample (3.8 mg/mL TbMORN1(7-15) and 2.6 mg/ml TgMORN1(7-15)) were injected onto a Superdex 200 10/300 (GE Healthcare) column and the flow rate was set to 0.5 ml/min ...
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bioRxiv - Biophysics 2021Quote: ... pH 7 using PD 10 columns (GE Healthcare) and diluted to a final concentration of 20 μM ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
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bioRxiv - Biochemistry 2021Quote: ... or incubated with 1 ml Ni2+ slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) per 1 L expression medium for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Sepharose™ 6 10/300 GL resin (30 cm x 1 cm; GE Healthcare) was equilibrated in PBS buffer (10 mM orthophosphate ...