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Citations for GE Life Sciences :
401 - 450 of 2379 citations for 6 methyl 2 thioxo 2 3 dihydroquinazolin 4 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the cells were resuspended in one mL R10 (RPMI [GE Healthcare Life Sciences ...
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bioRxiv - Bioengineering 2021Quote: ... and on one 5 ml StrepTrap HP column (GE Healthcare, US) equilibrated in binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... at a dilution of 1:10,000 for one hour (GE Healthcare). Proteins were detected using SuperSignal West Femto substrate (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... and one Discovery MR750 (General Electric system, GE Healthcare, United States) using a 32-channel head coil in Umeå ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by purification via Superose 6 (GE Healthcare). The proteins were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2021Quote: ... IgG Sepharose 6 Fast Flow beads (GE Healthcare) were used ...
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bioRxiv - Immunology 2021Quote: ... or pH 6-11 (GE Healthcare, Uppsala, Sweden). With IPG strip reduction ...
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bioRxiv - Neuroscience 2019Quote: ... 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% sarkosyl (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... or IgG Sepharose (6 Fast Flow, GE Healthcare) (500 μl per 500 ml culture) ...
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bioRxiv - Microbiology 2021Quote: ... Ni Sepharose 6 Fast flow resin (GE Healthcare) affinity exclusion chromatography was performed to obtain the tagless protein (containing the tag C-terminal sequence ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA flux 6 system (GE Healthcare, USA). The system and cassette were sanitized with 1.0 N NaOH before and after diafiltration ...
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bioRxiv - Biochemistry 2023Quote: ... or Superpose 6 10/300 GL (GE Healthcare) size exclusion column ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-mm-diameter Whatman paper discs (GE Healthcare) were placed on top of the plate and wetted with 5 μl of a 10 mM TcmX solution in DMSO or 300 μg of erythromycin diluted in 10 μl of ethanol ...
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bioRxiv - Immunology 2023Quote: ... Superose 6 Increase 10/300 GL (GE Healthcare) in a mobile phase of PBS containing 300 parts per million of sodium azide and at a flow rate of 0.75 mL/min (Dionex Ultimate 3000 pump ...
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bioRxiv - Synthetic Biology 2024Quote: ... IMAC Sepharose 6 Fast Flow beads (GE Healthcare) was added into (Ribosome purification ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... filter and subjected to Superose 6 (GE Healthcare) Increase column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed three times in TBST before incubation with HRP-conjugated secondary antibodies for 1hr at room temperature in 2% milk TBST (1/10,000, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... concentrated to a volume of 2 ml and applied onto a S75 16/600 pg size exclusion column (GE Healthcare), equilibrated with 10 mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 200 mM-300 mM NaCl in 20 mM HEPES (pH 7.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected and incubated with 2 column volume (for 20 mL supernatant) of Gluthatione Sepharose 4B (GE Healthcare) for 1h30 on a rotating wheel ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted protein sample was dialyzed against 20 mM Tris-HCl (pH 8.0) and 2 mM CaCl2 (buffer B) and applied to a Mono Q 10/100 GL column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... was expressed in E.Coli Rosetta 2 (DE3) and purified by affinity chromatography over a Sepharose 4B GSH affinity column (GE healthcare) followed by gel filtration over a Hiload Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Ycf1p NBD1 and NBD1-R were exchanged into the fluorescence buffer (20 mM Na3PO4, 2% glycerol, 150 mM NaCl, pH 7.3) by size exclusion chromatography (Superdex75, GE Healthcare). MgATP-bound and apo NBD1 and NBD1-R were generated by adding 2 mM MgATP or 2 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was applied to a Source 15 Q anion exchange column and eluted with a gradient of 150 mMà350 mM NaCl in 20 mM Immidazole (pH 8.0) and 2 mM DTT followed by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Nbs and their mixture (1.6 nmol of SARS-CoV-2 RBD mixed with 1.6 nmol of Nbs) were run over a Superdex 75 column (GE healthcare) at a flow rate of 0.5 mL/min with AKTA pure.
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids encoding for GST fusion proteins of Cobl-like1-411 were generated by subcloning into pGEX-4T-2 (GE Healthcare). A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... the coating was done with a 2:1 ratio of non-coupled:Cy3-coupled fibronectin prepared with Cy3 Mono-Reactive Dye Pack (GE Healthcare) as recommended by the manufacturer.
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... Clear lysates were filtered through 0.45 μm and immediately applied at 2 mL/min onto a HisTrap HP 5 mL column (GE Healthcare) previously equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... MRPP1 and 2 formed a complex of ~150 kDa that was further purified by gel-filtration (Superdex 200, GE Healthcare). His6-Δ95 MRPP3 cDNA lacking the N-terminal disordered region was cloned into pMCSG7 vector and expressed and purified ...
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bioRxiv - Cell Biology 2019Quote: U-2 OS FUCCI cells were grown on a 96-well glass bottom plates (Whatman, Cat# 7716-2370, GE Healthcare, UK ...
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bioRxiv - Immunology 2020Quote: ... 7.5×104 congenically distinct cells of each sorted memory population were adoptively transferred and tumor-bearing mice were subsequently immunized with 10 μg of GP33-41 and 2 μg of poly(I:C) (GE Healthcare) s.c ...
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bioRxiv - Genetics 2019Quote: Germ cells and embryos were mounted on 2% agarose pads and imaged live using a DeltaVision Elite microscope (Applied Precision) with a 60x Silicon oil objective lens ...
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bioRxiv - Biochemistry 2021Quote: All samples were prepared in a total volume of 40 μL in SEC buffer A supplemented with 2 mM DTT and analysed on a Superdex 75 3.2/300 Increase column (GE Healthcare). For the TTP-DCP2 interaction ...
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bioRxiv - Immunology 2021Quote: ... the protein was diluted with 2-fold volume of 20 mM HEPES with pH 7.0 and loaded on HiTrap SP HP column (GE Healthcare) equilibrated with 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... fragments or Fabs 2/1.12 or 2/6.14 were either used as analytes or amine immobilized on a CM5 chip in a Biacore 3000 (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: Human isoform of amphiphysin 2/BIN1 including exon 11 was expressed in Rosetta 2 bacteria and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50 mM Tris at pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... Images were taken at 2 second intervals for 1 minute and deconvoluted with Applied Precision softWorx imaging software (GE Healthcare). The microtubule comet tracking and quantification was performed with the MTrackJ plugin in ImageJ/Fiji (Meijering et al. ...
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bioRxiv - Biophysics 2022Quote: ... the complex was further purified by anion exchange chromatography in presence of 2% CHAPS using the AKTA system (GE Healthcare), flash frozen and stored at −80 °C until use.