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Citations for GE Life Sciences :
401 - 450 of 1663 citations for 6 hydroxy 2 naphthalenesulfonic acid sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Immunology 2021Quote: ... The bound protein was eluted with 100 mM acetic acid on ÄKTA pure (GE healthcare). To remove IgG1 Fc ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was purified on a 5ml nickel nitrilotriacetic acid (Ni2+-NTA) resin (GE Healthcare). The column was washed stepwise with 20 ...
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bioRxiv - Cell Biology 2023Quote: ... as a model of pre-adipocytes [30] were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (Nacalai ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2020Quote: ... α-Syn fibrils were formed by buffer exchange of purified monomers into fibril forming buffer (20mM Sodium phosphate, pH7.5) using a PD-10 column (GE Healthcare). The resulting α-Syn solution was passed through a 0.2 µm syringe filter ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 ml of 20 µM purified Sup35NM were buffer exchanged into Fibril Forming Buffer (FFB - 20 mM sodium phosphate buffer pH 7.4, 50 mM NaCl) using a PD-10 column (GE Healthcare) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding 50 mM sodium succinate (pH 5.0) followed by buffer replacement by using Column PD 10 desalting column (GE Healthcare). Antibody SN-38 drug conjugates were prepared using purified antibody [30] ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0) overnight at 4°C and further purified with Resource-S cation exchange column (GE Healthcare). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... AcrB was then buffer exchanged to buffer C (50 mM sodium phosphate, 150 mM NaCl, 10 % Glycerol, pH 7.4) using a PD-10 desalting column (GE Healthcare) and flash frozen at -80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Biochemistry 2023Quote: ... in SEC buffer (20 mM Sodium-phosphate buffer pH 6.5, 50 mM NaCl) using an ÄKTA pure chromatography system (GE Healthcare). Eluted fractions were concentrated using Amicon® Ultra to 1 mM final concentration.
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bioRxiv - Biochemistry 2023Quote: ... resolved by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electro-transferred on 0.45 μM nitrocellulose membranes (GE Healthcare). The membranes were incubated in blocking solution (5% skim milk in 0.05% Tween-20/Tris Buffered Saline ...
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bioRxiv - Cell Biology 2024Quote: Samples were separated electrophoretically on a 12.5% sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE) and transferred to Polyvinylidene Difluoride (PVDF, GE Healthcare) membranes at 60 mA per membrane for 90 min in a semi-dry transfer unit (TE77X Hoefer) ...
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bioRxiv - Biochemistry 2020Quote: ... and were subsequently applied to a Superose 6 10/300 size-exclusion column (GE healthcare) equilibrated in SEC buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was injected onto a Superose 6 10/300 size exclusion column (GE Healthcare) pre-equilibrated overnight with buffer containing 25 mM K-HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 150 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The sample was loaded on a 6 ml Resource Q column (GE Healthcare Life Sciences) equilibrated with 50 mM HEPES-NaOH pH 7.5 and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Concentrated complex was loaded onto a Superose 6 increase 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by size exclusion chromatography on a Superose 6 Increase 3.2/300 column (GE Healthcare) mounted on an ÄKTA Ettan (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were injected through a Superose 6 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... Eluted proteins were concentrated on Amicon concentrators and passed through a superose-6 (GE Healthcare) gel-filtration column in lysis buffer using a Bio-Rad NGC system ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted complexes were purified to homogeneity by injection on Superose 6 16/50 (GE Healthcare) column that was equilibrated in gel filtration buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... a second purification step on a nickel-column (Ni Sepharose 6 Fast Flow, GE Healthcare) was performed ...
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bioRxiv - Biochemistry 2021Quote: ... prior to injection on a Superose 6 Increase 10/300 GL column (GE Healthcare, SG) in buffer (20 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... MBP-ParCCTD was loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer.
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bioRxiv - Biochemistry 2020Quote: ... and further purified using size exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in SEC buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and further purified using size exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) at 4 °C in SEC buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was passed through a column of 6 mL Ni-Sepharose resin (GE Healthcare) at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Nanodiscs were then concentrated and purified on a Superose 6 10/300 GL (GE Healthcare) equilibrated with 0.2 M ammonium acetate ...
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bioRxiv - Cancer Biology 2020Quote: ... The labeled complex was further purified on a Superose 6 size exclusion column (GE Healthcare). For crystallography ...
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bioRxiv - Microbiology 2021Quote: ... full-length gB was further purified on a Superose-6 column (GE Healthcare Life Sciences) into TBS pH7.4 to remove aggregates.
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Size-exclusion chromatography was performed using a Superose 6 10/300 GL column (GE Healthcare) equilibrated in buffer 20 mM MES ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was further polished on XK 26/70 Superose 6 pg column (GE Healthcare) equilibrated in 20 mM Tris-HCl pH 8 (4 °C) ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was bound to pre-equilibrated Ni Sepharose 6 Fast flow resin (GE Healthcare). The beads were washed with 1xPBS (pH 7.4) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the supernatant was loaded onto 5 mL Nickel Sepharose 6 fast flow resins (GE Healthcare) in a gravity flow column ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC-HPLC) was carried out using a Superose-6 column (GE Healthcare). The protein concentration was 10-30 μM ...
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bioRxiv - Cell Biology 2019Quote: ... and separated on a Superose 6 Increase column (GE Healthcare Life Sciences, Chicago, IL, USA) on an NGC Chromatography system (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was concentrated and further purified by size exclusion chromatography (Superose 6, GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...