Labshake search
Citations for GE Life Sciences :
1 - 50 of 5457 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
-
bioRxiv - Immunology 2020Quote: ... and 0.1 M 3-(N,N-dimethylamino) propyl-N-ethylcarbonadiimide (GE healthcare) at a flow rate of 5 µL/min ...
-
bioRxiv - Plant Biology 2019Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
-
bioRxiv - Plant Biology 2021Quote: ... separated on 2 % agarose gels and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 2 mM EDTA) and electroblotted onto a nylon (N+) membrane (GE Healthcare RPN203B) at 20 V for 90 min at 4 °C in 0.5x TBE buffer ...
-
bioRxiv - Plant Biology 2020Quote: ... and 2 mM EDTA at pH 7.4) and blotted onto Hybond-N+ membrane (GE Healthcare) using a Minifold I slot blot system (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... RNA (2 μg) of each group was spotted on a Hybond-N+ membrane (GE Healthcare), followed by UV cross-linking (120 mJ/cm2 ...
-
bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
-
bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
-
bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5 N NaOH) and 2 h with transfer buffer (1.5 M NaCl, 0.25 N NaOH) using a VacuGene XL (GE Healthcare). The DNA was fixed to the membrane by soaking it in freshly prepared 0.4 N NaOH for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... purified RNA was electrophoresed in non-denaturing 2% agarose TBE gel and then gel was blotted onto an Hybound-N nylon membrane (RPN303N, GE Healthcare) by semidry electroblotting in 0.5× TBE buffer for 2 hours at 200mA ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
-
bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
-
bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
-
bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
-
bioRxiv - Molecular Biology 2020Quote: ... Hybond-N+ (GE Healthcare). A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG ...
-
bioRxiv - Neuroscience 2020Quote: ... injection of 185 MBq [123I]-2ß-carbometoxy-3ß-[4-iodophenyl]-N-[3-fluoropropyl]nortropane ([123I]FP-CIT, GE Healthcare, Amersham, UK). The SPECT data acquisition was begun after exactly 3 hours ...
-
bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
-
bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
-
bioRxiv - Genetics 2020Quote: ... at 60 x magnification (Figs. 2 and 4) or DeltaVision microscope (GE Healthcare) at 100 x magnification (Fig ...
-
bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
-
bioRxiv - Neuroscience 2019Quote: ... P2 fractions were pre-cleared in NP-40 buffer for 4 hours and then incubated o/n at 4 °C with protein A-Sepharose beads (GE Healthcare, Milan, Italy) conjugated to 10 μg/ml of anti-Pin1 antibody (Santa Cruz Biotechnology ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
-
bioRxiv - Plant Biology 2019Quote: ... Hybond-N+ (GE Healthcare, Chicago, USA), was placed on the MS plate with 1% agar ...
-
bioRxiv - Microbiology 2021Quote: ... Hybond-N membranes (Amersham/GE Healthcare) were used for Southern blots (45) ...
-
bioRxiv - Biochemistry 2021Quote: ... and Amersham Hybond-N+ (GE Healthcare) membranes were equilibrated in 1X membrane wash buffer (20 mM Tris-Cl ...
-
bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... and STD samples were mixed and the rehydration solution (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% IPG Buffer 4-7 [GE Healthcare]) was added before overnight IPG-strips passive rehydration (pH gradient 4-7 ...
-
bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: Dynamic PET/CT imaging (2-3h) with arterial blood sampling was performed on Monkey 3 and Monkey 4 using a Discovery MI (GE Healthcare). Each animal had two baseline scans which were separated by one month for Monkey 3 and by one year for Monkey 4 ...
-
bioRxiv - Genetics 2019Quote: ... Asci were stained with DAPI (4’,6-diamidino-2-phenylindole) and visualized by the DeltaVision Core Imaging System (Applied Precision, LLC, USA).
-
bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was supplemented with 4 mM CaCl2 and stirred for 2 h in 4°C with CaM-Sepharose beads (GE Healthcare), pre-equilibrated with binding buffer (50 mM Tris/HCl pH 7.2 ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: ... blotted onto Hybond-N+ membranes (GE Healthcare) and hybridized to biotin-labeled Sμ and σδ ...
-
bioRxiv - Microbiology 2019Quote: ... using Hybond N membranes (Amersham/GE Healthcare) at a hybridization temperature of 45°C.
-
bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS)-Sepharose beads (GE Healthcare) were crosslinked with recombinant proteins according to the manufacturer’s instructions and were used for the purification of antibodies against DJ-1 protein ...
-
bioRxiv - Developmental Biology 2020Quote: ... transferred onto Hybond N membranes (GE Healthcare), and probed with 32P-random-primed 1kb XmaI-EcoRV cDNA fragment corresponding to the exon 1-4 T-box region.
-
bioRxiv - Molecular Biology 2023Quote: ... transferred to Hybond-N+ membrane (GE Healthcare), and hybridized over-night at 50 °C in ULTRA-Hyb Oligo Hybridization buffer (Invitrogen AM8663 ...
-
bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
-
bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
-
bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
-
bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
-
bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 pl DNA solution was loaded onto Hybond N+ nylon membranes (GE Life Sciences) presoaked with PBS ...
-
bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...