Labshake search
Citations for GE Life Sciences :
151 - 200 of 3736 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 carboxyphenyl α 2 propyn 1 yl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
-
bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
-
bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
-
bioRxiv - Plant Biology 2023Quote: ... The probe was labelled with [α-32P] dCTP (BRIT, India) using the Rediprime random DNA labelling system (GE healthcare). The prehybridization ...
-
bioRxiv - Microbiology 2022Quote: ... and 1 mM 2-mercaptoethanol and refolded on a NiFF (GE Healthcare) column using a reverse linear urea gradient with imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
-
bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
-
bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
-
bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... 360562 RCF spin in a Ti70 rotor and incubated with 2 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare) for 2 h ...
-
bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
-
bioRxiv - Biochemistry 2019Quote: ... Metal chelate chromatography was performed at 4 °C using an IMAC zinc-Sepharose 6 Fast Flow column (2.6 × 20 cm, GE Healthcare Life Sciences, Amersham, UK) equilibrated with 1×PBS pH 6.0 ...
-
bioRxiv - Biochemistry 2019Quote: ... Following dialysis the E215C K490 construct was reacted for 4 h at 4°C with Cy3-maleimide (GE Healthcare, PA13131) at a motor/Cy3 dye ratio of 1:10 as previously described (Tomishige et al ...
-
bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 27.000 xg for 30 min at 4 °C the supernatant was incubated with 4 mL Ni2+-Sepharose resin (GE Healthcare) for 1 hour at 4 °C under gentle agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... and for additional 4 hours with 40 µl of pre-washed Protein G Sepharose 4 Fast Flow beads (GE Healthcare) in a rotating wheel ...
-
bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
-
bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
-
bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
-
bioRxiv - Molecular Biology 2019Quote: ... and glutathione Sepharose 4 Fast Flow (GE Healthcare), respectively ...
-
bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
-
bioRxiv - Microbiology 2021Quote: ... Wells were washed and probed with 100 μl/well of a goat α-GST antibody (GE Healthcare Life Sciences, 27457701V) diluted 1:800 and incubated for one hour at room temperature ...
-
bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was then purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). The fractions containing α-Synuclein were dialyzed overnight against water and dried after aliquoting 200 ug into low binding Eppendorf tubes ...
-
bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was further purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). Exclusively monomeric α- Synuclein was obtain by size exclusion chromatography using a HiLoad® 26/600 Superdex® 75 prep grade (from GE healthcare) ...
-
bioRxiv - Biophysics 2024Quote: ... The secondary antibodies used were a sheep α-mouse antibody conjugated to horseradish peroxidase (HRP) (GE Life sciences, Marlborough MA) at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
-
bioRxiv - Biophysics 2021Quote: ... Nesprin-4 and Nesprin-4-WD/AA were purified via size exclusion chromatography using Superdex 200 10/300 GL (GE Healthcare) column equilibrated in lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Diluted chromatin corresponding to 30-40 million cells was precleared overnight at 4°C with vertical rotation at 15rpm with 80uls of Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads and immunoprecipitated overnight with 40uls of beads and 5ug of antibody (13402-1-AP ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared at 26,200g for 30 min at 4°C and supernatants were incubated with pre-washed Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 90 min at 4°C with mixing ...
-
bioRxiv - Molecular Biology 2021Quote: ... which were supplemented with KCl to a final concentration of 100 mM were subjected directly to immunoprecipitation and incubated for overnight at 4 °C with antibodies and protein G-Sepharose 4 Fast Flow (GE Healthcare) or HA magnetic beads (Pierce) ...
-
bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
-
bioRxiv - Biophysics 2020Quote: ... The harvested supernatant was diluted with buffer A to a final DDM concentration of 2 mM and loaded to Ni2+-Sepharose™ 6 fast flow resin (GE Healthcare, already equilibrated with 10 CV buffer A with 780 μM DDM ...
-
bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
-
bioRxiv - Microbiology 2023Quote: ... HRP-linked F(ab’)2 fragment (GE Healthcare NA9340, from donkey, 1:5000) and Mouse IgG ...
-
bioRxiv - Biophysics 2021Quote: ... 25 µM of purified SpyCatcher-GFP was mixed with excess purified SpyTag-SAT09 (1:1.5) and incubated at 4°C for 1 hr and loaded on a HiPrep Desalting Column (GE Healthcare) equilibrated with 10mM NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: The binding affinity of the discovered antibodies for α-cobratoxin was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). All measurements were performed at 25 °C using 10 mM HEPES ...
-
bioRxiv - Cancer Biology 2021Quote: Primary MEFs isolated from E13.5 embryos from Ku70+/+ and Ku703A/3A mice were cultured in Hyclone α-MEM (GE Life Sciences) supplemented with 10% fetal bovine serum (Corning ...
-
bioRxiv - Molecular Biology 2019Quote: ... α-Syn was purified by size exclusion chromatography using a HiLoad Superdex column 16/600 75 pg (GE healthcare life sciences) connected to an ÄKTA system (GE healthcare life sciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... Relative levels of [α-32P]-labelled GTP and GDP were detected and quantitated with Typhoon phosphor-imager (GE Healthcare, NSW, Australia).
-
bioRxiv - Evolutionary Biology 2021Quote: ... Protein A-Sepharose 4 Fast Flow suspension (GE Healthcare) was washed with Tris-Buffered Saline (TBS ...
-
bioRxiv - Genomics 2020Quote: ... Protein G Sepharose beads (GE Healthcare, 4 Fast Flow) were washed with WB140 ...
-
bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
-
bioRxiv - Molecular Biology 2021Quote: ... and Protein A SepharoseTM 4 Fast flow (GE Healthcare) beads by dimethyl pimelimidate cross-linking as described in (69).
-
bioRxiv - Molecular Biology 2021Quote: ... nProtein A Sepharose 4 Fast Flow beads (GE Healthcare) were used ...
-
bioRxiv - Biochemistry 2022Quote: ... and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare), respectively ...
-
bioRxiv - Biochemistry 2021Quote: ... or Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) following the instructions provided by the manufacturer with appropriate modifications ...
-
bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...