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Citations for GE Life Sciences :
1 - 50 of 2762 citations for 6 NITRO 1 PHENYL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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bioRxiv - Biophysics 2022Quote: ... hydrophobic chromatography on a phenyl-sepharose column (HiTrap Phenyl HP, GE Healthcare) and size exclusion chromatography on an analytical HiLoad 16/60 Superdex 75 (HiLoad 16/600 Superdex 75 pg ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... HiTrap Phenyl HP (GE Healthcare, 17-1351-01) hydrophobic column was used to obtain β-glucosidase fraction ...
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bioRxiv - Neuroscience 2020Quote: ... Separated samples were transferred to a nitro-cellulose membrane (GE Healthcare). Following transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Separated proteins were transferred to a nitro-cellulose membrane (GE Healthcare). Following transfer ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at room temperature (GE Healthcare, NA934V, donkey anti-rabbit, 1:1000) a 5 min incubation with chemiluminescent HRP substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... followed by another purification using Phenyl Sepharose (GE Healthcare, USA).
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bioRxiv - Developmental Biology 2022Quote: ... the gel content was transferred on 0.45µm nitro-cellulose membranes (GE Healthcare). After blocking with 5% milk and PBS/NP40 (PBS+0.3% NP40) ...
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bioRxiv - Immunology 2021Quote: ... and hydrophobic interactions with a HiTrap Phenyl HP column (GE healthcare. The individual TCR δ- and γ-chains of the VD1G9 TCR were ordered (IDT) ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was applied to a Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MES-NaOH pH 6.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... CaM was initially purified by affinity to phenyl sepharose (GE Life Sciences) in the presence of 5 mM CaCl2 ...
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bioRxiv - Immunology 2023Quote: ... Phenyl-Sepharose Fast Flow (low sub) (GE Healthcare Life Sciences, Uppsala, Sweden) was added and supernatants gently mixed in 4°C for 1.5 h ...
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bioRxiv - Cell Biology 2019Quote: ... Phenyl-Superose HR5/5 and Superose 12 HR10/30 were from GE Healthcare.
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bioRxiv - Biophysics 2022Quote: ... the solution was loaded onto a 10 ml Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... the solution was loaded onto a 10 ml Phenyl-sepharose HP (GE Healthcare) column equilibrated with 20 mM MOPS-NaOH pH 6.7 ...
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bioRxiv - Biochemistry 2023Quote: ... hydrophobic interaction chromatography (HIC) on a 5-ml Phenyl-Sepharose column (GE Healthcare), and gel filtration on a Superdex 75 16/600 (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Cell Biology 2023Quote: ... 1H were collected using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5).
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bioRxiv - Microbiology 2020Quote: ... The cell free supernatant was loaded onto a 5 ml HiTrap Phenyl FF (GE Healthcare) and protein was eluted by using a linear reverse gradient to 100 mM NaCl over 90 min at a flow rate of 2.5 ml/min ...
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bioRxiv - Biochemistry 2019Quote: ... protein supernatant was bound to a phenyl sepharose column (High Load FF 16/10, GE Healthcare). The column was washed with buffer C and protein eluted with 30% ethanol in water ...
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bioRxiv - Biochemistry 2020Quote: ... 1.5 M (NH4)2SO4) and was loaded on a 50 ml Phenyl Sepharose column (GE Healthcare). After one column volume wash step the tRNAs were eluted with a linear gradient of Phe-sepharose B buffer (20 mM NaOAc ...
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bioRxiv - Biophysics 2022Quote: ... A second purification step was performed by hydrophobic interaction chromatography (HiTrap Phenyl HP column, GE Healthcare), where OCP was eluted in 40 mM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was finally subjected to hydrophobic interaction chromatography using a phenyl Sepharose column (GE Healthcare) equilibrated in buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified using an AKTA FPLC system with HiTrap-Phenyl-HP column (GE Healthcare) and Superdex 200 size exclusion chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... StuI was further purified by hydrophobic interaction chromatography in a HiTrap Phenyl HP column (GE Healthcare). The ion-exchange chromatography were performed as a linear gradient from 20 mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydrophobic interaction chromatography (HIC) using a 5 mL HiTrap Phenyl FF (HS) column (GE Healthcare). For the ion exchange procedure ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was loaded on a HiLoad 16/10 Phenyl Sepharose HP (GE Healthcare® Life Sciences) column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The resulting supernatant was checked for activity prior to being applied to a 75mL Phenyl Sepharose (GE Healthcare) resin equilibrated in Buffer B (1 M ammonium sulfate ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently incubated for 1h at RT with anti-mouse (NA931, GE Healthcare) HRP-conjugated secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Alpha-synuclein was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction column (GE Healthcare) (76) ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled and supplemented with ammonium sulfate to a final concentration of 1.5M and loaded on a 5ml pre-packed HiTrap Phenyl HP column (GE Healthcare) equilibrated with buffer A (100mM Tris pH 7.3 ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Plant Biology 2021Quote: ... was added to the supernatant to dilute the ammonium sulfate concentration to 0.5 M and the solution was applied to pre-equilibrated hydrophobic column after passing through a 0.22 μm filter (Hitrap phenyl, GE Healthcare). Proteins were eluted by an ammonium sulfate gradient from 0.5 M to 0 M with ten column volumes ...
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bioRxiv - Biochemistry 2021Quote: ... a hydrophobic interaction chromatography using a linear decreasing (NH4)2SO4 gradient (from 1.5 M to 0 M) (HiTrap HIC phenyl column, GE Healthcare) was applied to the mixture ...
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bioRxiv - Biophysics 2020Quote: ... the pH of the aSyn sample was adjusted to pH 7 and filtered through a 0.22 μm filter before being loaded onto a HiPrep Phenyl FF 16/10 (High Sub) column (GE Healthcare, Sweden) and eluted in HIC buffer A (50 mM Bis-Tris ...
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bioRxiv - Biophysics 2019Quote: ... aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare). aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Neuroscience 2022Quote: ... 50 mM Bis-Tris pH 7) and further purified on a HiPrep Phenyl FF 16/10 (High Sub) hydrophobic interaction chromatography (HIC) column (GE Healthcare) and eluted against buffer D (50 mM Bis-Tris pH 7) ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Biochemistry 2021Quote: ... or incubated with 1 ml Ni2+ slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) per 1 L expression medium for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Sepharose™ 6 10/300 GL resin (30 cm x 1 cm; GE Healthcare) was equilibrated in PBS buffer (10 mM orthophosphate ...
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bioRxiv - Neuroscience 2020Quote: ... 1h at room temperature with the secondary antibody and developed using an ECL prime solution (GE Healthcare, RPN2232V1).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
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bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...