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Citations for GE Life Sciences :
101 - 150 of 3076 citations for 6 Chloro 5 methoxypyridin 2 amine hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... 2 mM CaCl2 to load onto an HiTrap Q HP 5 ml column (GE Healthcare). After anion exchange purification ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Synthetic Biology 2022Quote: CM3 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Immunology 2020Quote: CM5 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Biophysics 2019Quote: ... 360562 RCF spin in a Ti70 rotor and incubated with 2 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5) was immobilized on the surface of a CM5 sensor chip using a Amine Coupling Kit (GE Healthcare Bio-Sciences AB, Sweden). VHHs were serial 4-fold diluted from 417 nM to 0 nM and flowed over the captured RBD surface at 15 μl/min for 3 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
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bioRxiv - Genetics 2019Quote: ... Asci were stained with DAPI (4’,6-diamidino-2-phenylindole) and visualized by the DeltaVision Core Imaging System (Applied Precision, LLC, USA).
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bioRxiv - Biophysics 2020Quote: ... The harvested supernatant was diluted with buffer A to a final DDM concentration of 2 mM and loaded to Ni2+-Sepharose™ 6 fast flow resin (GE Healthcare, already equilibrated with 10 CV buffer A with 780 μM DDM ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Biochemistry 2019Quote: ... Superose-6 Increase (GE Healthcare) or Superdex S-200 (GE Healthcare ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotide pool was purified using 2 x illustra NAP-5 Columns (GE Life Sciences, PN: 17085302). The resulting solution was evaporated to dryness ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used ...
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bioRxiv - Neuroscience 2022Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used.
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bioRxiv - Biochemistry 2020Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used.
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bioRxiv - Synthetic Biology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used ...
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bioRxiv - Immunology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used (Supplemental Figure 2).
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bioRxiv - Biochemistry 2022Quote: ... After Superose 6 (GE Life Sciences) size-exclusion chromatography ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto a nickel (II) 5 mL HisTrap packed with Sepharose® 6 fast flow agarose beads (GE Healthcare Biosciences, NSW, Australia) using an ÄKTApure™ system (GE Healthcare Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Biochemistry 2022Quote: ... and size-exclusion (Superose 6; GE Healthcare) chromatography ...
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bioRxiv - Immunology 2020Quote: ... and Superose 6 (GE Healthcare Life Sciences) size exclusion chromotography (SEC) ...
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bioRxiv - Immunology 2020Quote: ... and a Superose 6 column (GE Healthcare). Before sample loading ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 increase 10/300 (GE Healthcare) was utilized for analysis of gephyrin and LS variants complexes ...
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bioRxiv - Biophysics 2023Quote: ... Sepharose 6 Fast Flow beads (GE Healthcare) were packed in a column (packed volume 10 L ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA Flux 6 (GE Healthcare, USA) were used for buffer exchange in this unit process step ...
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bioRxiv - Biophysics 2023Quote: ... and size-exclusion (Superose-6; GE Healthcare) chromatography.
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...