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Citations for GE Life Sciences :
1 - 50 of 3165 citations for 6 Chloro 5 fluoro 1H indole 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Cy3B NHS ester (GE Healthcare); ATTO 647N NHS ester (ATTO-TEC) ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Genomics 2023Quote: ... Cy3B–NHS ester (GE Healthcare PA63101), or Alexa Fluor 488–NHS ester (Invitrogen A20000 ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... mounted with a Superose 6 5/150 increase column (GE Healthcare) equilibrated in size-exclusion chromatography buffer (20 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2019Quote: ... Using Cy5-NHS ester (GE Healthcare, PA15101) yielded 54-70% labeling ...
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bioRxiv - Systems Biology 2020Quote: ... or Cy3B-NHS ester (GE Healthcare PA63101) or Alexa Fluor 488-NHS (Invitrogen A20000 ...
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bioRxiv - Microbiology 2019Quote: ... Cy5 mono-reactive NHS ester (GE Healthcare), Alexa488 NHS ester (Thermo Fisher) ...
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bioRxiv - Systems Biology 2021Quote: ... or Cy3B-NHS ester (GE Healthcare PA63101) or Alexa Fluor 488-NHS ester (Invitrogen A20000 ...
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bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2019Quote: LecB was labeled with fluorescent dyes bearing NHS esters as reactive groups (Cy3 mono-reactive NHS ester (GE Healthcare), Cy5 mono-reactive NHS ester (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 5 mL Ni2+ Sepharose 6 Fast Flow (GE Healthcare) was transferred to a 20 mL gravity flow column ...
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bioRxiv - Molecular Biology 2019Quote: ... with Cy5-NHS-ester purchased from GE Healthcare. Synthesis and purification of activated Ala-and Val-DBE (3,5-dinitrobenzyl esters ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling with Cy5-NHS ester (GE Healthcare, PA15101) yielded 54–70% labeling efficiency ...
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1H were collected using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5).
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bioRxiv - Biochemistry 2022Quote: ... 5 ml of Ni2+-beads slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) were washed in washing buffer (20 mM TrisHCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Analytical SEC was performed using Superose 6 5/150 GL column (GE Healthcare) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... A Superose 6 Increase 5/150 size exclusion chromatography column (GE Healthcare Lifescience) was used to remove thrombin and exchange the buffer to αCSF90 buffer (70 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa-488 or Alexa-568 NHS esters (GE Healthcare) as described previously49.
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Biophysics 2022Quote: Cy3B-NHS ester (PA63101) was purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were labeled with Cy3 NHS ester (GE Healthcare) at an amine-modified internal thymine and HPLC purified on a 218TP C18 reverse-phase column (Grace/Vydac ...
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Molecular Biology 2019Quote: ... using the NHS-ester form of Cy dyes (GE Healthcare). 2012).1 μL of 1 mM of DNA/RNA dissolved in MilliQ H20 is added to 5 μL labeling buffer of (freshly prepared ...
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bioRxiv - Biophysics 2019Quote: ... a stock solution of Cy3B (monoreactive NHS ester, GE Healthcare) was prepared by dissolving a small amount of solid in 1 mL of doubly-distilled water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the supernatant was loaded onto 5 mL Nickel Sepharose 6 fast flow resins (GE Healthcare) in a gravity flow column ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Molecular Biology 2019Quote: ... purified using size-exclusion chromatography using a superose 6 increase 5/150 column (GE Healthcare) in crosslinking buffer (50 mM HEPES pH 8 ...
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bioRxiv - Immunology 2022Quote: ... The protein was fractionated by size-exclusion chromatography (Superose 6 increase 5/150, GE Healthcare) with buffer F (20 mM HEPES at pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and eluted from a Superose 6 Increase 5/150GL size exclusion column (GE Healthcare, UK) in 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Neuroscience 2020Quote: ... were coupled with Cy3B mono-reactive NHS ester (PA63101, GE Healthcare) according to the supplier’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... an Amersham Cy3 mono-reactive NHS ester dye pack (GE healthcare) was resuspended with labeling buffer and mixed with enough purified APE1 to yield a 100 μL solution of 10 mg/mL APE1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Amersham Cy5.5 Mono NHS Ester was from GE Healthcare (Buckinghamshire, UK), Amicon Ultracel membranes (10 kDa ...
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bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...