Labshake search
Citations for GE Life Sciences :
551 - 600 of 3108 citations for 6 Chloro 2 methylimidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
-
bioRxiv - Microbiology 2021Quote: ... The MxiN and Spa33 were combined with an excess of MxiN and the mixture was concentrated to a final volume of 2 mL for injection onto a Hi Load 26/600 Superdex 200 pg column (GE Healthcare Life Sciences). The protein absorbance at 280 nm was monitored to detect protein elution on an AKTA system (GE Healthcare Life Sciences) ...
-
bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... DNA was stained with 0.2 µg/ml ethidium bromide (EtBr) (Nacalai Tesque, 14631-94) and detected using a Typhoon FLA9000 gel imaging scanner (GE Healthcare, Chicago, Illinois). Gel images were processed using ImageJ 1.8.0 (NIH ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
-
bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
-
bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
-
bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
-
bioRxiv - Neuroscience 2023Quote: ... A 20-μl droplet of virus inoculum containing 8X106 plaque-forming unit (PFU) of PRV-Becker, resuspended in medium (Dulbecco modified Eagle medium, 2% fetal calf serum and antibiotics) (Hyclone, GE Healthcare life sciences), was applied onto the abraded area of the skin ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
-
bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
-
bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
-
bioRxiv - Cell Biology 2023Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
-
bioRxiv - Microbiology 2024Quote: ... MUC5B was purified using a Bio-Rad NGC fast protein liquid chromatography (FPLC) system equipped with an XK 50 column packed with 2 L of Sepharose CL-2B resin (GE Healthcare Bio-Sciences). Mucin-containing fractions were identified using a periodic acid-Schiff’s reagent assay and analysis of UV absorbance at 280 nm from FPLC ...
-
bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
-
bioRxiv - Bioengineering 2019Quote: ... 2 mL of human buffy coat/fresh blood mixed well with 2mL of phosphate-buffed saline (PBS) and then carefully layered on top of 2 mL of Ficoll (GE Healthcare Life Sciences, US) inside a 10ml centrifuge tube without mixing ...
-
bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
-
bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
-
bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
-
bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... applied onto a Superdex 6 column (10/300 Increase, GE Healthcare) and eluted in a buffer containing 25 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2019Quote: ... Size exclusion chromatography (Superose-6 increase 10/300 column, GE Healthcare) was then performed in Gel Filtration Buffer with a Shimadzu High Performance Liquid Chromatograph ...
-
bioRxiv - Cancer Biology 2021Quote: ... Ni sepharose 6 Fast flow (GE Healthcare, New York, NY, USA) was applied for the purification of EXOSCs ...
-
bioRxiv - Molecular Biology 2020Quote: A Superose 6 column (GE Healthcare 17-5172-01 Lot# 10225796) was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare) ...
-
bioRxiv - Biophysics 2019Quote: ... IMAC Sepharose 6 Fast Flow Resin was acquired from GE Healthcare Bio-Sciences AB ...
-
bioRxiv - Biochemistry 2019Quote: ... RNAP was further purified using size exclusion (Superose 6, GE Healthcare) and ion-exchange chromatography (ResourseQ column ...
-
bioRxiv - Biochemistry 2021Quote: ... Ni sepharose 6 FastFlow beads were obtained from GE Healthcare (USA). Anion exchange Chromatography was performed using ÄKTA pure system (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... 600 μl of IgG Sepharose® 6 Fast Flow (GE healthcare) bead suspension was taken into a column and washed once with 10 ml of IP binding buffer (20 mM HEPES-KOH pH 7.9 ...
-
bioRxiv - Biophysics 2020Quote: ... The complexes were further purified over Superose 6 increase (GE healthcare) equilibrated with 25 mM HEPES pH 7.9 ...
-
bioRxiv - Biophysics 2020Quote: ... Size exclusion chromatography (Superose-6 increase 10/300 column, GE Healthcare) was then performed in Gel Filtration Buffer with a Shimadzu High Performance Liquid Chromatograph ...
-
bioRxiv - Biochemistry 2022Quote: ... and SEC using a Superose 6 10/300 column (GE Healthcare) as described49.
-
bioRxiv - Biophysics 2022Quote: ... and then loaded onto a Superose 6 increase column (GE Healthcare) on an NGC system (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... pH 6) and loaded onto a DEAE Sephacel column (GE healthcare) equilibrated with equilibration buffer ...
-
bioRxiv - Biochemistry 2020Quote: SEC-HPLC was performed using a Superose-6 column (GE Healthcare). The protein concentration was 20-30 μM ...
-
bioRxiv - Cancer Biology 2022Quote: The SuperoseTM 6 10/300 GL gel filtration column (GE Healthcare) was equilibrated with one column volume of running buffer (20mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2022Quote: ... then loaded onto a Superose 6 10/300 increase (GE Healthcare) column for gel filtration in GF buffer buffer (20 mM Tris-HCl ...
-
bioRxiv - Immunology 2019Quote: ... The complex was then purified over Superose 6 column (GE Healthcare) and concentrated to 1.5 mg/mL and mixed with Lauryl Maltose Neopentyl Glycol (LMNG ...
-
bioRxiv - Microbiology 2020Quote: ... pH 6) and loaded onto a DEAE Sephacel column (GE healthcare) equilibrated with buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... except that a Superose 6 increase (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
-
bioRxiv - Biochemistry 2020Quote: Sample was injected onto Superose 6 10/300 column (GE Healthcare) at a flow rate of 0.20mL/min in size-exclusion buffer (20mM HEPES ...
-
bioRxiv - Molecular Biology 2019Quote: ... mounted with a Superose 6 5/150 increase column (GE Healthcare) equilibrated in size-exclusion chromatography buffer (20 mM Tris-HCl pH 8 ...