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Citations for GE Life Sciences :
151 - 200 of 4125 citations for 6 Bromo N tert butyl 2 4 chlorophenyl imidazo 1 2 a pyridin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... siRNA non-targeting pool #2 (GE Healthcare Dharmacon) was used as a control for all the knockdown experiments ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... (2) Ni Sepharose fast flow resin (GE Healthcare) chromatography using a step elution from 50 mM to 200 mM Imidazol in Tris buffer B (20 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare, Vélizy-Villacoublay, France), 0.5% penicillin-streptomycin (Invitrogen)) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM ATP) overnight at 4°C and loaded onto a 2-ml Glutathione-Sepharose 4B (GE Healthcare Life Sciences, Piscataway, NJ, USA) column pre-charged with GST-N-WASp-VCA (Nolen & Pollard ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mg of each of the remaining lysates was diluted four-fold with IP buffer plus 0.1% v/v Triton X-100 and incubated for 2 hr at 4 °C with protein G-Sepharose (GE Healthcare catalog no. 17061801), pre-bound to HA-specific antibody (rabbit anti-HA from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were immobilized using direct immobilization using amine coupling on CM3 sensor chips (GE Healthcare) at ∼5000 RU ...
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bioRxiv - Biophysics 2019Quote: ... DNA modified with a primary amine group was mixed with Cy3 NHS ester (GE Healthcare) at room temperature for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples of both male and female livers from all the different treatments (N=6-8) were individually used to isolate total RNA via the Illustra RNAspin Mini RNA Isolation kit (GE Healthcare), according to the manufacturer’s standard protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Solubilized supernatant was isolated by ultracentrifugation and the supernatant was incubated for 2 h with 2 mL CNBR-activated Sepharose beads (GE healthcare) coupled with 4 mg high-affinity GFP nanobodies (2) ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Systems Biology 2021Quote: ... and imaged with an IN Cell Analyzer 6000 (nine fields of view per well, 20x/0.45NA air objective, 2൸2 binning) (GE Healthcare Life Sciences). Following image acquisition ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... was directly immobilized on the CM5 chip using an amine coupling kit (GE Healthcare Life Sciences). Before immobilization ...
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bioRxiv - Cell Biology 2021Quote: ... was amine coupled to the carboxy-methylated dextran surface of a CM5 sensor chip (GE Healthcare) using standard amine coupling chemistry ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... the sensor chip was saturated with streptavidin using an amine coupling kit (GE Healthcare Life Sciences). Biotinylated pMHCs were immobilize at various levels (100–300 RU for kinetics ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 2 ml of Chelating Sepharose Fast Flow (GE Healthcare) was added to a small plastic column and prepared for affinity purification by sequential washing with 1 column volume (CV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2 mM DTT using PD10 columns (GE Life Sciences) and concentrated to 9 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT by HiTrap desalting column (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... 4°C (SORVALL RC 6+) and supernatant was applied to a HisTrap affinity column (GE Healthcare) that was pre-equilibrated with binding buffer ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions eluted from anionic exchange column were incubated with ammonium sulfate to a final concentration of 1M and loaded on a Butyl Sepharose High Performance HiLoad16/10 (GE Healthcare) equilibrated with buffer C (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 4°C overnight with primary antibody (1:1000) and incubated for 1 hour at RT with secondary antibodies in either 2% PBA (GE healthcare, RPN418), 5% ELK or OBB (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1-2 minutes in darkness then exposed and imaged using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... sortase and excess DBCO-amine was removed using Ni-NTA resin and PD-10 columns (GE Healthcare) respectively ...
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bioRxiv - Biophysics 2020Quote: ... Neutravidin (Pierce) was amine coupled to the carboxymethylated dextran surface of a CM5 sensor chip (GE Healthcare) using standard amine coupling chemistry ...
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bioRxiv - Biophysics 2020Quote: The SPR chip was prepared by amine coupling of a polyclonal rabbit anti-mouse IgG (GE Healthcare) on both flow cells of CMD500 chip (Xantec) ...
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bioRxiv - Biophysics 2019Quote: ... A drop of 5 μL of 2% dimethyldichlorosilane (GE Healthcare) was added on the mask and hard vacuum was applied for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Flow cell (FC) 2 of an NTA chip (GE Healthcare) was activated with 30 μl 0.5 mM NiCl2 ...
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bioRxiv - Genetics 2022Quote: ... 2 ul of Streptavidin Sepharose High Performance beads (GE Healthcare) and 35 ul of H2O ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 2% of Protease Inhibitor Mix (GE Healthcare, Uppsala, Sweden). We purified all samples using a 2-D Clean-Up Kit (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM β-mercaptoethanol) on an ÅKTA system (GE Healthcare). PfKinesin-8X-MD containing fractions were pooled and loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare ...