Labshake search
Citations for GE Life Sciences :
301 - 350 of 3883 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
-
bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
-
bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
-
bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
-
bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
-
bioRxiv - Biophysics 2022Quote: ... the volume was reduced to a volume of 400 ml using the ÄktaFlux system with a 3 kDa MWCO cartridge (GE Healthcare). Afterwards the system was used for buffer exchange with 50 mM HEPES ...
-
bioRxiv - Cell Biology 2019Quote: Each oligonucleotide contained an amino group at the 3’ end for fluorophore coupling using either Cy5 NHS ester (GE Healthcare, PA15102) or rhodamine NHS ester (ThermoFisher ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2020Quote: ... 5’-FAM-UGUUCUUCUGGAAGUCCAGTT-3’) was separated by gel electrophoresis (20% polyacrylamide gel containing 7 M Urea) and visualized with a Typhoon FLA9500 (GE Healthcare).
-
bioRxiv - Molecular Biology 2021Quote: ... The reaction took place for 3 h and the radio labeled kCR probe purified in a Sephadex G25 column (Amersham, GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... Purified 6-His-PI3Kγ catalytic subunit17,18 was used as the soluble analyte and was injected at different concentrations over the immobilized GRK2 in SPR buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% surfactant P-20, GE Healthcare Life Sciences, Biacore Inc.). Binding experiments were performed at 25°C in SPR buffer with a flow rate of 20 μl/min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
-
bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
-
bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
-
bioRxiv - Biophysics 2021Quote: FH1FH2 constructs of Cdc12p (residues 882-1375) and Bni1p (residues 1227-1776) were expressed from pGEX-4T-3 plasmids (GE Healthcare) in BL21(DE3 ...
-
bioRxiv - Cell Biology 2020Quote: ... N571 and N442 in frame with N-terminal tag glutathione S-transferase (GST) in pGEX-6P-3 expression plasmids (GE healthcare) were expressed in BL21-CodonPlus-RP E ...
-
bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
-
bioRxiv - Immunology 2022Quote: ... ELISA plates were afterwards washed 3 times with PBST and 50 μL of anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (Cytiva, GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
-
bioRxiv - Neuroscience 2022Quote: ... apposed them to a BAS-SR2040 phosphor screen (Fujifilm) for 3 days and imaged the slides using a phosphorimager (Typhoon FLA 7000; GE Healthcare). We drew ROIs onto the sections with standards using Multigauge software (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... The samples were then applied to IPG strips and separated in the first dimension with the IPGphor 3 system from GE Healthcare according to their isoelectric focusing (20 C ...
-
bioRxiv - Cell Biology 2019Quote: ... and 3’-primary amino-modified miR-430 MO (5’-TCTACCCCAACTTGATAGCACTTTC-3’) was obtained from Gene tools LLC and labeled with Cy3 NHS-ester (GE Healthcare). After the conjugation for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2019Quote: ... The cell-free supernatant was diluted 1:1 with LA buffer (50 mM lactic acid, pH 3) and applied to a 5 ml HiTrap SP Sepharose column (GE Healthcare). The column was washed by applying a pH gradient from 100 % LA buffer to 100% H buffer (50 mM HEPES-NaOH ...
-
bioRxiv - Microbiology 2019Quote: ... were loaded on 7-cm long immobilized pH gradient (IPG) strips with non-linear (NL) 3-10 pH gradient (GE Healthcare). Isoelectric focusing was carried out in Multiphor II electrophoresis system (GE Healthcare ...
-
bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
-
bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...
-
bioRxiv - Pathology 2019Quote: ... proteins were separated based on their isoelectric point on 18-cm immobilized pH gradient (IPG) gel strips of pH 3-10 (GE Healthcare). Subsequent to overnight rehydration ...
-
bioRxiv - Cell Biology 2019Quote: A total of 1.5 mg each of 0 hr and 3 or 5 hr ssDNA samples were labeled with Cy3-dUTP or Cy5-dUTP (GE Healthcare) by random priming without denaturation with 4 mg random nonamer oligo (Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2020Quote: Computerized axial tomography scanning (CT scan) was performed on anesthetized P30 mice [3% of Isoflurane (Isoba Vet)] using an eXplore ista PET-CTscan (GE Healthcare) with the following parameters ...
-
bioRxiv - Cell Biology 2020Quote: ... antigen was CENP-AHCP-3(105-183)::6xHis) and detected using an HRP-conjugated secondary antibody (rabbit or mouse; GE Healthcare). For antibody production CENP-AHCP-3(105-183)::6xHis was expressed in E ...
-
bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2021Quote: ... 50 mM NaCl) at approximately 3 mg/mL and then further purified by size-exclusion chromatography using a Superdex-200 column (GE Healthcare) equilibrated in solubilization buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the pirA gene was cloned into XhoI and EcoRI sites of pGEX-4T-3 vector (GE Healthcare Life Sciences, Freiburg, Germany), encoding recombinant PirA with N-terminal-fused GST-tag ...
-
bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated for 3 h at room temperature and loaded onto a Superdex 200 10/300 GL (GE Healthcare) sizeexclusion chromatography column using buffer B as mobile phase ...
-
bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
-
bioRxiv - Synthetic Biology 2022Quote: ... then wrapped into a clear foil and exposed in the cassette with the phosphor screen for 3 days and visualized on a Phosphorimager (Typhoon FLA 7000, GE Healthcare). prDC1416 was used to generate the blot in Fig ...
-
bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... control HUVEC or HUVEC 48 hr post-siRNA treatment were incubated with cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ml fibrinogen (820224 ...
-
bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
-
bioRxiv - Neuroscience 2023Quote: All scans were conducted in a 3 Tesla General Electric scanner with an eight-channel head coil (GE MR750; GE Healthcare). We acquired anatomical images via T1-weighted ...
-
bioRxiv - Microbiology 2024Quote: ... The eluted Cas1-Cas2/3 complex was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer C ...
-
bioRxiv - Biophysics 2024Quote: ... His6-GB1-tag was subsequently cleaved by TEV protease (3 mM) and separated from bZIP on a cation exchange column (HiTrap SP; GE Healthcare) using a 0–2 M gradient of sodium chloride ...
-
bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
-
bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... 360562 RCF spin in a Ti70 rotor and incubated with 2 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare) for 2 h ...
-
bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
-
bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
-
bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2019Quote: ... Each probe contains a 3’-end amine-modified nucleotide that allows directly couple to an NHS-ester Cy3 dye (GE Healthcare, PA23001) according to the manufacturer’s instructions ...