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Citations for GE Life Sciences :
1 - 50 of 1525 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Physiology 2024Quote: ... utilizing a tandem arrangement of 2 Superose 6 columns (GE Healthcare). The elution process entailed the collection of fractions in 0.5 ml increments ...
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bioRxiv - Cell Biology 2023Quote: ... 1H were collected using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5).
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-CAPN7(MIT)2 was bound to GST-sepharose beads (10 mL,GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at room temperature (GE Healthcare, NA934V, donkey anti-rabbit, 1:1000) a 5 min incubation with chemiluminescent HRP substrate (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification column was prepared using 2-3 mL IMAC Sepharose 6 Fast Flow beads (GE Healthcare) and charged with 0.1 N Nickel sulphate solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently incubated for 1h at RT with anti-mouse (NA931, GE Healthcare) HRP-conjugated secondary antibody ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ml of media was concentrated over 2 ml heparin affinity medium (Heparin Sepharose 6 Fast Flow, GE Healthcare) packed into a 10 ml Poly-Prep Chromatography gravity flow column (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: LC3B (amino acids 1-120) was expressed in BL21 pLysS DE3 E.coli cells from a pGEX-6P-2 plasmid (GE Healthcare) in 250 ml LB medium ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots (2 µl) of the homogenate were used for protein determination with a BCA (bicinchoninic acid) assay kit (GE Healthcare). Samples were prepared with 5X sample buffer (0.25 M Tris-HCl pH6.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... the combined peptide containing solutions were passed over pre-equilibrated (50 µl 0.5 % formic acid ) home-made 2-disc Glass microfiber StageTip (disc material: GE Healthcare; pore size ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Neuroscience 2020Quote: ... 1h at room temperature with the secondary antibody and developed using an ECL prime solution (GE Healthcare, RPN2232V1).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Immunology 2022Quote: ... 500 μL of the proteins ranging from 2 mg/mL – 4 mg/mL concentration were loaded on an analytical Superose 6 Increase 10/300 column (GE Healthcare) and eluted in 1x PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of 100% ethanol or 10% DEET in ethanol was applied to a 2 cm x 6 cm strip of Whatman filter paper (GE Healthcare). The treated paper was hung from a rectangular plastic frame in front of the Peltier element ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
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bioRxiv - Developmental Biology 2020Quote: ... The protein were electrophoresised for 1h at 150V and subsequently transferred onto MtOH activated polyvinylidene fluoride membranes (GE Healthcare). Equal loading was assessed using Ponceau red solution ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The harvested supernatant was diluted with buffer A to a final DDM concentration of 2 mM and loaded to Ni2+-Sepharose™ 6 fast flow resin (GE Healthcare, already equilibrated with 10 CV buffer A with 780 μM DDM ...
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bioRxiv - Molecular Biology 2021Quote: ... and analyzed (normalization, spot matching, expression analyses, and statistics) using Image Master 2-D Platinum 6 image analysis software (GE Healthcare, USA). Spots on the gel were identified and each spot was assigned a spot quantity (q) ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was obtained post the ultracentrifugation at 175,000 g for 1h and loaded onto a HiTrap TALON crude column (GE Healthcare) for affinity enrichment ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was recovered and incubated for 2 h with pre-equilibrated Ni-NTA beads (Ni Sepharose 6 Fast Flow, GE Healthcare Life Sciences) in a binding buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used ...
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bioRxiv - Neuroscience 2022Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used.
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bioRxiv - Biochemistry 2020Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used.
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bioRxiv - Synthetic Biology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used ...
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bioRxiv - Immunology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used (Supplemental Figure 2).
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bioRxiv - Biochemistry 2022Quote: ... After Superose 6 (GE Life Sciences) size-exclusion chromatography ...
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bioRxiv - Synthetic Biology 2022Quote: Protein cargo exchange experiments were performed by incubating 100 nM DNA origami with 300nM Alexa-647 labelled protein cargo for 1h before addition to washed s300 resin (according to manufacturer’s instructions, GE Life Sciences) in a spin column and centrifugation for 4 mins at 1000 g to remove free protein ...