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Citations for GE Life Sciences :
351 - 400 of 4046 citations for 6 Amino 4 hydroxy 3 7 sulfo 4 4 sulfophenyl azo 1 naphthyl azo naphthalene 2 7 disulfonic acid sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and subjected to size exclusion chromatography at 4°C using a Superdex-200 column (GE Healthcare) and SEC buffer (50 mM Bis-Tris-Propane pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Equilibrium dissociation constants were obtained at 4°C using a MicroCal VP-ITC system (GE Healthcare). Peptide concentrations between 300-750 μM in the syringe were injected into 25–35 μM pocket domain in the cell ...
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bioRxiv - Biochemistry 2019Quote: ... The purified nanobody was then coupled to CNBr-Activated Sepharose 4 Fast Flow beads (GE Healthcare) to yield the GFP nanotrap ...
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bioRxiv - Plant Biology 2019Quote: ... Ligands were coupled to NHS-activated Sepharose™ 4 Fast Flow beads (GE Healthcare, United Kingdom). Control beads blocked with ethanolamine were prepared in the same way ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... D1-4 (CNFY1-704) was purified using a HiLoad 16/600 Superdex 200 pg (GE Healthcare) pre-equilibrated in buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... the media was passed through a Sepharose 4 fast flow gel filtration column (GE Healthcare, 170149001), using the AKTA pure chromatography system (GE Health care) ...
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bioRxiv - Molecular Biology 2019Quote: ... and then analyzed by size-exclusion chromatography at 4°C using a AKTAmicro system (GE Healthcare) mounted with a Superose 6 5/150 increase column (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... samples were digested with 4 µL Sequencing Grade Modified trypsin (0.5 µg/µl; GE Life Sciences) overnight at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Genetics 2021Quote: ... Cells were then disrupted by sonication and after centrifugation (30 min at 30000 × g at 4°C) the supernatant (soluble fraction) was purified by FPLC on a 1 ml HisTrap FF column (GE Healthcare; Milan, Italy) by stepwise elution ...
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... The mixtures were incubated at 4°C for 1 h prior to purification on a Superdex 200 Increase SEC column (GE Healthcare Life Sciences) in buffer containing 150mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were incubated at 4°C for at least 1 hr with ∼40 µL of appropriate bead slurry (nProtein A Sepharose, GE Healthcare, 5280-01). Following incubation ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and with KCl to a final concentration of 0.3 M and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 7 ml of 30% Percoll (GE Healthcare, Catalogue no.-17-0891-01), and centrifuged at 931g for 8 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... His-SEPT2-mCherry-SEPT6/7-strep was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Physiology 2023Quote: ... Transthoracic echocardiographic images were obtained using a Sonosite Micromaxx (BCF Technology) or Vivid 7 (GE Healthcare) with short-axis 2D views at the mid-papillary muscle level recorded[32] ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was nutated (2h, 4°C) with 10ml glutathione-Sepharose 4B resin (GE Healthcare, Pittsburg, PA) in resuspension buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biochemistry 2021Quote: Aβ42 monomers were immobilized by amine coupling onto flowcell 4 on a CM5 sensor chip (GE Healthcare) using a BIAcore 3000 instrument (BIAcore AB) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were re-adsorbed onto a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Plant Biology 2022Quote: ... crude extracts (4 ml) were passed through 0.5 ml DEAE-Sephadex A-25 columns (GE Healthcare, USA). The bound GSL was digested on the column for 18 h at room temperature with ∼0.8U myrosinase/thioglucosidase ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...