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Citations for GE Life Sciences :
301 - 350 of 3311 citations for 6 7 Dihydro 2 formyl 5H pyrrolo 1 2 a imidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... or a Superose 6 increase (Superose 6 Increase 10/300 GL, GE Healthcare) which were equilibrated in SEC buffer ...
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bioRxiv - Systems Biology 2019Quote: ... 0.7% (v/v) acetonitrile (ACN) and 1% (v/v) carrier ampholytes mixture (IPG buffer pH 3.0-10.0, GE Healthcare). Fractionation was performed on a 3100 OFFGEL (OGE ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl, 20 mM NaH2PO4, 500 mM imidazole) on an ÄKTA Pure chromatography system (GE Healthcare). Fractions containing the protein were pooled ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was eluted with IMAC buffer containing 330 mM imidazole and loaded onto a HiTrap SP HP column (GE Healthcare). The column was washed with ion exchange buffer (IEC ...
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bioRxiv - Biochemistry 2020Quote: ... AcrB was then eluted with Buffer B containing 500 mM imidazole and directly injected onto a Superdex 75 10/600 GL size exclusion chromatography (SEC) column (GE Healthcare) equilibrated in buffer D (50 mM sodium phosphate ...
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bioRxiv - Biophysics 2021Quote: ... 500 mM imidazole) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL column, GE Healthcare). Protein concentration is determined using the protein extinction coefficient and corrected for direct absorption of the fluorophores at 280 nm ...
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bioRxiv - Biophysics 2021Quote: ... The dialyzed media was filtered again and 5 mM imidazole was added to the media before loading onto a Histrap column (GE healthcare). The protein was eluted using a linear imidazole gradient (20 to 500mM ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was eluted with loading buffer containing 400 mM imidazole and then purified by gel filtration with a Superdex 200 column on the ÄKTAprime plus system (GE Healthcare) in binding buffer (20 mM Tris-Cl ...
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bioRxiv - Neuroscience 2019Quote: ... the protein was eluted using 400 mM Imidazole (5 CV) and loaded onto a HiLoad 16/600 Superdex 200 pg size exclusion column (GE Healthcare). The main peak was collected ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove soluble aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer A (devoid of ATP and PefaBloc but containing 200 mM instead of 30 mM imidazole) and further purified over a Superdex 200 10/300 GL column (GE Healthcare) in buffer B (25 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... Samples were eluted using buffer A containing 300 mM imidazole and were subsequently applied to a MonoQ 10/100GL column (GE Healthcare) and eluted using a linear NaCl gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Capsid protein was eluted at 1 M imidazole in NTE buffer and the collected elute was subjected to size exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare). The fraction containing DENV2C protein were pooled and dialyzed against 50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... His-Thrombin-TEV-Nsp15 variants were eluted with 250 mM imidazole and further purified by gel filtration on a Superdex-200 column (GE Healthcare) in SEC Buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The his-tagged protein was eluted 4 CV of elution buffer (lysis buffer supplemented with 250 mM imidazole) and further purified by size exclusion chromatography in a Superdex 200 10/30 (GE Healthcare) equilibrated with 20 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Biophysics 2020Quote: ... 500 mM imidazole) The labelled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex 75 Increase 10/300 GL, GE Healthcare) to remove remaining fluorophores and aggregates ...
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bioRxiv - Biochemistry 2021Quote: ... and eluted with an elution buffer (20 mM NaK, 0.5 M NaCl, and 0.5 M imidazole) in an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The buffer of the eluted proteins was replaced with a mesotrypsin buffer (100 mM tris(hydroxymethyl)aminomethane (Tris) ...
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bioRxiv - Immunology 2021Quote: ... was used to elute the protein using a binding buffer with 400 mM imidazole followed by size exclusion chromatography on a Superdex75 16/600 (GE Healthcare) column equilibrated in binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was eluted with 240 mM imidazole and further purified over a Superdex S-75 gel filtration column (GE Healthcare) equilibrated in 150 mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... pre-equilibrated with five volumes of imidazole binding buffer (PBS, 10 mM imidazole buffer, pH 7.4) using a fast protein liquid chromatography (FPLC) system (AKTA Pure; GE Healthcare systems). The column was washed with twenty bed volumes of binding buffer (PBS ...
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bioRxiv - Genomics 2020Quote: ... Proteins were eluted with 3mL elution buffer (PBS, 250mM Imidazole) and excess salt was removed using the desalting column according to the manufacture’s protocol (GE Healthcare, 17085101). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
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bioRxiv - Microbiology 2021Quote: ... The peak fraction (eluting at ~150 mM imidazole) was pooled and further purified over size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 8.0 and 150 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... We eluted bound proteins using an imidazole gradient and subjected them to size-exclusion chromatography (Superdex 200 16/600, GE Healthcare) using 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... and the protein was eluted with an imidazole gradient (300 mM-500 mM) using the AKTA prime chromatography system (GE Healthcare). Fractions containing pure ParR protein (26.5 kDa ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Microbiology 2023Quote: ... After elution with 500 mM imidazole the VHH-containing fractions were buffer-exchanged with PBS using a Vivaspin column (5 kDa cutoff, GE Healthcare). The purified VHHs were analyzed by SDS-PAGE and Coomassie staining and by intact mass spectrometry ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... MBP-CENP-E2605-2701 was purified using the same lysis buffer without imidazole and an MBP-Trap HP coloumn (GE Healthcare). For ITC ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were eluted with a lysis buffer containing 400 mM imidazole and further purified with size exclusion chromatography (Superdex 200 column, GE Healthcare) with a buffer containing 50□mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM BME) with elution buffer B (buffer A with 500mM Imidazole) on a 5mL His-Trap FF Crude Nickel Column (GE Healthcare). Fractions containing Pol θ were pooled and were separated further on a 5mL HiTrap Heparin HP (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Imidazole and NaCl present in the Ni-NTA running buffer were eliminated by using a HiTrap Desalting 5 mL (GE Healthcare) column.
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bioRxiv - Biochemistry 2024Quote: ... and low-salt buffer (200 mM NaCl) the protein was eluted with 500 mM imidazole and further purified using size-exclusion chromatography (Superose S12 column; GE Healthcare).
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 400 mM imidazole and concentrated and applied to Superdex 200 increase 10/300 GL column (GE Healthcare) equilibrated in 0.2 M sodium bicarbonate buffer (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: His6-Ras1.G17V (1-172) was purified using Ni Sepharose 6 Fast Flow (GE Healthcare) and dialysed into buffer A (20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... The gels were transferred for 2 hours onto nitrocellulose membranes (GE Healthcare Biosciences, Pittsburgh, PA). Detection was performed by diluting primary antibodies in TBS supplemented with 0.1% Tween and either 5% BSA or 5% non-fat dry milk ...
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bioRxiv - Physiology 2019Quote: ... Protein concentration was determined using commercially available 2-D quant kit (GE Healthcare Life Sciences). Sample quality was confirmed using NuPAGE® protein gels stained with fluorescent SyproRuby protein stain (Thermo Fisher).
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bioRxiv - Genomics 2019Quote: ... The flow-through was then loaded onto a 2 mL HiTrap SP column (GE Healthcare) in 17% IEX-B buffer ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mM NaCl and 2 mM CaCl2) by PD-10 desalting column (GE Healthcare, USA). The cleavage reaction was performed with hEKL (370 nM ...
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bioRxiv - Biochemistry 2020Quote: ... loaded in a gravity column containing 2 mL of pre-equilibrated Ni-sepharose (GE Healthcare), and incubated under slow rotation for 30 min at 8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...