Labshake search
Citations for GE Life Sciences :
51 - 100 of 4218 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... 1 × 20 mL (GE Healthcare) with loading buffer (10 mM Tris (pH 7.6) ...
-
bioRxiv - Neuroscience 2020Quote: Derivatized-protein samples were analysed by two-dimensional (2D) gel electrophoresis using Immobiline® DryStrips pH 3-11 (IPG) non-linear (NL) 24 cm (GE Healthcare) for isoelectric focusing (IEF ...
-
bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
-
bioRxiv - Microbiology 2020Quote: ... Swarmer cells were isolated from the culture by centrifugation (20 mins at 11,000 rpm, 4°C) after mixture with 1 volume of Percoll (GE Healthcare). The synchronized swarmer cells were pipetted onto an agarose (2% ...
-
bioRxiv - Biochemistry 2021Quote: ... The buffered supernatant was loaded on a DEAE-20 mL HiPrep FF 16/10 anion exchange chromatography column (GE Healthcare), equilibrated with Tris-HCl buffer (50 mM ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... Cellulose acetate membrane (0.2μm pore, GE Healthcare) was equilibrated in 0.1% SDS/H2O and fixed to the filter trap device (PR648 Slot Blot Blotting Manifold ...
-
bioRxiv - Microbiology 2020Quote: ... 4-20% (p/v) Amersham ECL Gel (GE Healthcare, Chicago, Illinois, EEUU), using the ECL Gel Box system (GE Healthcare ...
-
bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
-
bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
-
bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...
-
bioRxiv - Microbiology 2020Quote: ... Sgo_R10 and Sgo_R3-4 were further purified by preparative SEC using a Superdex 75 16/600 column (GE Healthcare) equilibrated in 20 mM Tris.HCl ...
-
bioRxiv - Immunology 2022Quote: ... Aliquots of the purified A48 or D48 PTX3 proteins were run under denaturing conditions on Tris-Acetate 3–8% (w/v) and Bis-Tris 10% (w/v) protein gels (GE Healthcare Life Sciences), in the absence and presence ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 x 20 mL (GE Healthcare) on an ÄKTA pure protein purification system (Cytiva ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 x 20 mL (GE Healthcare) on an ÄKTA pure protein purification system (Cytiva ...
-
bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
-
bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... Fractions containing the Mtw1c were concentrated using a Vivaspin 20 ultracentrifugal unit MWCO 10000 Dalton (Satorius) and loaded to a HiLoad Superdex 200 16/600 pg (GE Healthcare) equilibrated in 30 mM HEPES ...
-
bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
-
bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
-
bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
-
bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein containing fractions were concentrated to 3 mL and further purified by size exclusion using a HiPrep 16/60 Sephacryl S-500 HR (GE Healthcare) in Buffer D (20 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... Active HAP fractions eluting from 0.2–0.3 M KPi were pooled and separated on a Superose 6 XK 16/70 gel filtration column (130 ml, GE Healthcare) equilibrated with buffer D + 0.1 mM EDTA at 0.15 M KCl ...
-
bioRxiv - Microbiology 2023Quote: ... The eluted fractions were concentrated to 3 ml and applied to 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... and then the eluate was incubated overnight on a rotator at 4°C.The protein was resolved on a HiLoad 16/600 Superdex 200 Column (GE Healthcare) pre-equilibrated with storage buffer (20 mM HEPES-KOH ...
-
bioRxiv - Immunology 2021Quote: ... or pH 6-11 (GE Healthcare, Uppsala, Sweden). With IPG strip reduction ...
-
bioRxiv - Cancer Biology 2021Quote: ... 20 µL of Protein A Sepharose 4 FF (GE Healthcare Japan, Tokyo, Japan) was added and mixed with rotation at 4°C for 3 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... using 10mM sodium acetate pH4.0 solution (GE Healthcare). The surface of the sensor chip was blocked with 1M ethanolamine-HCl pH8.5 (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... The rest was incubated for 1 h at 4°C with 20 µl Ni Sepharose High Performance beads (GE Healthcare Life Sciences) to isolate the nanobodies ...
-
bioRxiv - Cell Biology 2020Quote: ... Fractions containing CLIK-1 were applied to HiLoad 16/60 Superdex 75 (GE Healthcare) and eluted with a buffer containing 0.1 M KCl ...
-
bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
-
bioRxiv - Plant Biology 2019Quote: ... and electrotransfered (2 h at 80 V or 16 h at 30 V and 4°C) onto polyvinylidene difluoride membrane (Amersham, GE Healthcare). The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen) ...
-
bioRxiv - Biochemistry 2019Quote: Peak fractions from the IEX were concentrated to < 4 ml and loaded onto a Superdex 16/60 gel filtration column (GE Healthcare) in Buffer A ...
-
bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
-
bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... The affinity-purified protein was TEV-cleaved o/n at 4°C and subsequently subjected to gel filtration on an equilibrated Superdex 75 gel filtration column 16/600 (GE Healthcare). Monomer fractions were pooled and concentrated to 1 mg/ml ...
-
bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Biochemistry 2019Quote: ... Concentrated protein was spun down 15 min in a bench-top centrifuge (4°C) and immediately loaded onto a HiLoad 16/600 Superdex 200 column (GE Healthcare), preequilibrated in gel filtration buffer consisting of 20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
-
bioRxiv - Biochemistry 2021Quote: ... spun at max speed for 30 min in a bench-top centrifuge (at 4°C) and finally loaded on a Superdex200 16/60 (GE Healthcare) previously equilibrated in MPS1 buffer (250 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... samples were concentrated in 10K MWCO centricons (PAL Corporation) (5000rpm [3214xg] at 4°C) and applied on a Superdex200 HiLoad 16/600 column (GE Healthcare) using 25 mM Hepes pH 7.6 ...
-
bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
-
bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... and UNC- 16(1-506) was covalently coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare).
-
bioRxiv - Biochemistry 2022Quote: ... 16/600 (GE Healthcare, Rosenberg, Sweden). The size exclusion chromatography as last step of the purification was performed directly in NMR buffer.
-
bioRxiv - Plant Biology 2022Quote: ... A cellulose acetate membrane filter (GE Healthcare Life Sciences) was placed in a slot blot apparatus (Bio-Rad ...