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Citations for GE Life Sciences :
151 - 200 of 1737 citations for 5 Sulfosalicylaldehyde sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... using the Amersham 5 AI600 (GE Healthcare, USA).
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bioRxiv - Neuroscience 2021Quote: ... After washing again for 5 x 5 min western blots were visualized with ECL plus Western Blotting detection system (#RPN2231, GE Healthcare) or Immobilon Forte Western HRP substrate (#WBLUF ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...
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bioRxiv - Biochemistry 2020Quote: ... and 5% (v/v) glycerol for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 50 mM to 1 M NaCl ...
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bioRxiv - Microbiology 2019Quote: ... and was chromatographed sequentially on a 5-ml HiTrap Q HP column and on a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare), which was equilibrated with MonoQ buffer A containing 50 mM NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... at 4 °C overnight and removed by passing the sample through a 5 ml Ni2+-NTA column connected to a 5 ml dextrin sepharose (MBPTrap) column (GE Healthcare), both equilibrated in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: Custom siRNAs targeting 5’-CACCAUGAGUGGCAGUCAG-3’ 33 for FRA1 and 5′-GUUCACUGCUGGCCUAUAA-3’ 34 for FLI1 were ordered from GE Healthcare Dharmacon ...
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bioRxiv - Epidemiology 2019Quote: ... measles MV-N was purified by chromatography on a MonoQ HR 5/5 strong anion exchange column (GE Healthcare, Piscataway, NJ) prior to use ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and then separated sequentially on a 5-ml HiTrap Q HP column followed by a 5-ml HiTrap SP FF column (GE Healthcare), which were both pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Microbiology 2023Quote: nFhGST was purified by affinity chromatography from a Fasciola hepatica adult fluke extract using a 5/5 GSTrap HP column (GE Healthcare) following a pre-established protocol9 ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Microbiology 2021Quote: Whole cell protein lysate from 1 × 106 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were blocked by incubation with 5% milk powder in PBS for 1 h at room temperature (RT ...
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bioRxiv - Microbiology 2022Quote: Fresh blood was collected from healthy donors in a sodium heparin vial and PBMCs were isolated by Ficoll-paque™ PLUS (GE healthcare) density gradient centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Microbiology 2022Quote: ... The resulting translated proteins were resolved by a sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) gel and analyzed by phosphoimager analysis (Typhoon, Amersham, GE life sciences).
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate, pH 7.2, 500 mM NaCl, 30mM imidazole) using an AKTA FPLC (GE Healthcare, Piscataway, NJ). The column was washed with 15 column volumes of binding buffer and protein was eluted in a linear gradient to 100% elution buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Immunology 2021Quote: ... the protein was reduced with DTT and buffer-exchanged into 100 mM sodium phosphate buffer using a Hi-Trap® desalting column (GE Healthcare). Reduced Hypocrates (0.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed of unbound probe with a pre-warmed (42°C) 2X saline-sodium citrate (SSC) buffer and detected by phosphor imaging (Storm™ 840, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... FhuFred was prepared using sodium dithionite and then its excess was removed through buffer exchange in a HiTrap® Desalting Column (GE Healthcare). The different concentrations were used to avoid and deal with the spectra overlap between FhuF and the Fe(III)-siderophores ...
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Molecular Biology 2023Quote: ... with 20 mM sodium phosphate (pH 6.5) and by size-exclusion chromatography on a HiLoad 16/60 Superdex 75 column (GE Healthcare Life Sciences) with 20 mM sodium phosphate (pH 6.5 ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The IgG anti-SARS-CoV-2 was purified from plasma of a convalescent patient using a 5/5 HiTrap rProtein-A column (GE Healthcare, USA). See detailed information about this procedure in Supplementary method No.1.
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bioRxiv - Microbiology 2022Quote: ... was supplemented with 4 mM MgCl2 and 5 mM imidazole and loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 0.1 % LDAO ...
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bioRxiv - Cell Biology 2021Quote: ... 5% fetal bovine serum (FBS; GE Healthcare, Chicago, IL), and 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were imaged using Typhoon 5 Imager (GE Healthcare) to record signals from the Cy5 dye ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare) was used for further purification which resulted in a monodisperse and clear INO80 complex ...
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bioRxiv - Biophysics 2019Quote: ... loaded onto a 5-mL HisTrap column (GE Healthcare) and finally eluted with 220 mM imidazole in buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... on a MonoQ 5/50 GL column (GE Healthcare). Sample purification and analysis with HPLC were performed using Agilent 1260 Infinity (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl samples of Protein A-Sepharose (GE Healthcare) were preincubated with 10 µl each of purified α-MyoE or α-Uso1 antibodies for 3 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µl of streptavidin beads (GE Healthcare, Chicago, USA) per O.D.254 unit was added ...
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bioRxiv - Bioengineering 2021Quote: ... a Sephadex G-25 column (GE Healthcare, NAP-5) was equilibrated with PBS (PH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... A HisTrap HP column (5 ml, GE Healthcare, France), prepacked with Ni High Performance Sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were scanned using a Typhoon 5 (GE Healthcare) and the signal intensity quantified using Image Studio Lite (LI-COR Biosciences).
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bioRxiv - Biochemistry 2022Quote: ... applied to a 5 ml HisTrap HP (GE Healthcare), and washed with lysis buffer using an Äkta pure fast-performance liquid chromatography system (GE Healthcare,) ...
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bioRxiv - Biochemistry 2020Quote: ... Anion exchange chromatography (MonoQ 5/50 GL, GE Healthcare, Buffer ...