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Citations for GE Life Sciences :
101 - 150 of 3155 citations for 5 Pyrimidinecarbonitrile 4 ethoxy 6 trifluoromethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Microbiology 2019Quote: ... 5-10 µg of proteins were resolved on 4–12% SDS-polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with various antibodies prior to development with ECL prime (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used ...
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bioRxiv - Neuroscience 2022Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 column (GE Healthcare) was used.
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bioRxiv - Biochemistry 2020Quote: ... HyClone Boost 6 supplement (GE Healthcare) was added with 10% of the end volume ...
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bioRxiv - Biochemistry 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used.
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bioRxiv - Synthetic Biology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used ...
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bioRxiv - Immunology 2020Quote: ... a Superose 6 Increase (GE healthcare) column was used (Supplemental Figure 2).
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bioRxiv - Biochemistry 2022Quote: ... After Superose 6 (GE Life Sciences) size-exclusion chromatography ...
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bioRxiv - Bioengineering 2023Quote: ... Clarified lysate was loaded onto a nickel (II) 5 mL HisTrap packed with Sepharose® 6 fast flow agarose beads (GE Healthcare Biosciences, NSW, Australia) using an ÄKTApure™ system (GE Healthcare Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed with 4% paraformaldehyde for 5 min and images were captured at room temperature on a DeltaVision fluorescence microscope (Applied Precision) installed with softWoRx version 5.5 housed in the Micron Oxford essentially as described [13] ...
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was removed by centrifugation at 10000xg for 45 min at 4°C and the supernatant was loaded onto either a 1 mL or 5 mL HisTrap FF crude column (GE Healthcare) equilibrated in Ni-1 buffer using a peristaltic pump ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Physiology 2021Quote: ... guinea pigs were anesthetized (4-5% isoflurane with 2L/min O2) and placed on a micro-PET scanner (GE eXplore Vista, GE Healthcare) fitted with a nose cone to inhale 2.5-3% isoflurane with 1L/min O2 for maintenance of anesthesia ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Biochemistry 2020Quote: ... After concentration by spin filtration to 4-5 mL pro-rhodesain was loaded onto a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), and purified by SEC (0.5 mL/min ...
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bioRxiv - Cell Biology 2022Quote: ... 5 min at 4 °C followed by an anion exchange chromatography loading the supernatant on a HiTrap Q HP 5 ml column (GE Healthcare) and intensive wash step of 20 column volumes with ion exchange buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... Aβ42 peptides in the filtrate were further concentrated to ∼50 μM at 4°C and 4000×g with a 5 000 Da Vivaspin concentrator (GE Healthcare). The Aβ42 peptide concentration was calculated using an extinction coefficient of 1400 M-1cm-1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Biochemistry 2022Quote: ... and size-exclusion (Superose 6; GE Healthcare) chromatography ...
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bioRxiv - Immunology 2020Quote: ... and Superose 6 (GE Healthcare Life Sciences) size exclusion chromotography (SEC) ...
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bioRxiv - Immunology 2020Quote: ... and a Superose 6 column (GE Healthcare). Before sample loading ...
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bioRxiv - Biochemistry 2022Quote: ... Superose 6 increase 10/300 (GE Healthcare) was utilized for analysis of gephyrin and LS variants complexes ...
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bioRxiv - Biophysics 2023Quote: ... Sepharose 6 Fast Flow beads (GE Healthcare) were packed in a column (packed volume 10 L ...
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bioRxiv - Bioengineering 2023Quote: ... and AKTA Flux 6 (GE Healthcare, USA) were used for buffer exchange in this unit process step ...
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bioRxiv - Biophysics 2023Quote: ... and size-exclusion (Superose-6; GE Healthcare) chromatography.
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The fusion protein was cleaved with 10% (w/w) TEV protease overnight at 4°C and the mixture was loaded on a 5 ml HisTrap™FF column (GE Healthcare). 6xHis-tag-labeled disulfide bond isomerase and TEV protease were retained ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Immunology 2023Quote: ... covered in 4+4 Whatman papers (GE Healthcare, UK) soaked in transfer buffer (192 mM glycine ...
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bioRxiv - Plant Biology 2021Quote: ... GFP or mCherry fluorescence was measured 4-5 days post-infiltration (see Table S3 for raw data) using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) with the appropriate excitation and emission settings (EX ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Cell Biology 2020Quote: ... followed by purification via Superose 6 (GE Healthcare). The proteins were frozen in liquid nitrogen and stored at −80 °C.