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Citations for GE Life Sciences :
101 - 150 of 3478 citations for 5 NAPHTHALEN 1 YL 2H PYRAZOL 3 YLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 3 ml Ficoll-Paque media (GE Healthcare) was pipetted into the bottom and the diluted 4 ml blood sample was carefully layered on top ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Biophysics 2019Quote: ... The reaction was incubated at 37°C for 2-3 hours on an orbital shaker and loaded onto an anion-exchange colum (1 ml DEAE FF, GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... Full-length coding regions were amplified and inserted in frame into pET-28a(+) (69864-3, MilliporeSigma) and/or pGEX-4T-1 (28-9545-49, GE Healthcare) to create N-terminal ...
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bioRxiv - Biophysics 2022Quote: ... The TOP3A-RMI1/2 complex was assembled by incubating purified TOP3A and RMI1/2 (1:3 ratio) followed by purification using a Superdex 200 Increase (GE Healthcare) in buffer I.
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging of one-cell embryos expressing CYB-1::mNG or mNG::CYB-3 was performed on a widefield deconvolution microscope (DeltaVision Elite; Applied Precision) equipped with a charge-coupled device camera (pco.edge 5.5 sCMOS ...
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Enrichments were performed in test tubes containing 5 mL of M63 minimal media and a 1 x 10 cm strip of Whatman #1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) pressed against the side of the tube (30) ...
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bioRxiv - Immunology 2019Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0-0.5 M NaCl in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and loaded onto a Mono Q HR 5/5 column (GE Healthcare Life Sciences). Desired tetramers were eluted using a linear gradient of 0–0.5 M NaCl in 20 mM Tris (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were washed for 5 min three times with TBS-T before incubating at room temperature for 1 h with the secondary antibody (anti-rabbit HRP conjugated anti-rabbit IgG [GE Healthcare] diluted 1:5,000 in 3% BSA/ TBS-T). After washing the membranes three times in TBS-T buffer ...
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bioRxiv - Genomics 2019Quote: ... The bioreactors were inoculated at a target cell density of 0.4 × 106 cells/mL in ActiPro batch media and fed daily beginning on Day 3 (3% (v/v) Boost 7a and 0.3% (v/v) Boost 7b (GE Healthcare)) ...
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bioRxiv - Biochemistry 2022Quote: ... and then immobilized in 7 cm IPG strips (pH 3-10 : 70×3×0.5 mm) and a linear gradient (NL) (GE Healthcare Immobiline™ Dry Strip IPG ...
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bioRxiv - Immunology 2023Quote: ... [3] A HisTrap FF column (Cytiva, GE Healthcare) was used in the Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Plant Biology 2024Quote: ... and subcloned into pGEX-4T-3 (GE Healthcare) using BamHI and SalI ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml volume (GE Healthcare), preequilibrated with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Cy®5 (GE Healthcare) anti-mouse secondary antibody was used ...
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bioRxiv - Immunology 2020Quote: SARS-CoV and SARS-CoV-2 ectodomain and RBD constructs were transiently transfected in Expi293F cells at a density of 2 million cells/mL using polyethylenimine and expression plasmids at a molar ratio of 3:1 and purified from clarified transfected culture supernatants 4-days post-transfection using Ni2+-NTA affinity chromatography (GE Life Sciences). Proteins were eluted from the column using 250 mmol/L imidazole ...
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bioRxiv - Microbiology 2023Quote: ... equivalent to ∼1-3×108 cells per sample) were collected by vacuum filtration onto glass microfiber filters (grade GF/A, GE Healthcare Whatman), immediately plunged into liquid nitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2019Quote: ... The water phase was loaded to a MonoQ 5/50 GL (1 ml) column (GE17-5166-01, GE Healthcare). ppGpp was eluted with a linear gradient from 0.5 mM to 600 mM LiCl in buffer C (25 mM Tris-HCl pH 8.3 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and maintained in a 4:1 ratio of DMEM (Dulbecco’s modified Eagle’s medium, GE Healthcare, Logan, UT) to Medium 199 containing 10% calf serum (HyClone ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2021Quote: ... and the cleaved DRH-3 CTD and DRH-3 NTD were purified using a Superdex 75 HiLoad 16/600 size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 5-15 mg ml−1 in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sample was then run on an 18 cm 3-10 strip using the Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare). Four voltage steps (50 V for 10 hours ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2023Quote: ... the lysates were adjusted to equal protein concentrations and incubated with 500 pmol of GST-14-3-3 immobilised on glutathione-sepharose 4B beads (GE Healthcare) for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluate was further purified by ion exchange chromatography (Mono Q 5/5, GE Healthcare). Peak fractions were pooled and protein purity was assessed by SDS–PAGE and Coomassie staining ...
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bioRxiv - Biophysics 2021Quote: ... followed by ion exchange purification using a mono-Q HR 5/5 column (GE Healthcare). Purified Nap1 was snap frozen in buffer containing 10 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...