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Citations for GE Life Sciences :
1 - 50 of 4524 citations for 4 6 Difluoro 1 methyl 1H indazole 5 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 10 mM acetic acid-NaOH pH 4.0 overnight at 4 °C and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 10 mM acetic acid-NaOH pH 4.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 1,200 rpm at 4°C for 6 minutes and the pellet was resuspended in 5 ml of 40% Percoll (GE Healthcare) in PBS and transferred to a 15 ml tube ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at room temperature (GE Healthcare, NA934V, donkey anti-rabbit, 1:1000) a 5 min incubation with chemiluminescent HRP substrate (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 5 mL Ni2+ Sepharose 6 Fast Flow (GE Healthcare) was transferred to a 20 mL gravity flow column ...
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bioRxiv - Neuroscience 2021Quote: ... using the 14N(p,α)11C route and converted to either [11C]MeI (methyl iodide) or [11C]MeOTf (methyl triflate) using a Tracerlab FX MeI module (GE Healthcare). A Tracerlab FX M module (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution, GE Healthcare, 1h at RT), with washing steps as above ...
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bioRxiv - Cell Biology 2023Quote: ... 1H were collected using a Deltavision Elite system (GE Healthcare) controlling a Scientific CMOS camera (pco.edge 5.5).
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bioRxiv - Biochemistry 2022Quote: ... 5 ml of Ni2+-beads slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) were washed in washing buffer (20 mM TrisHCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Analytical SEC was performed using Superose 6 5/150 GL column (GE Healthcare) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... A Superose 6 Increase 5/150 size exclusion chromatography column (GE Healthcare Lifescience) was used to remove thrombin and exchange the buffer to αCSF90 buffer (70 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biochemistry 2021Quote: 50 µL samples at 5-10 µM concentration were loaded onto a Superose 6 5/150 analytical size exclusion column (GE Healthcare) equilibrated in buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Analytical size-exclusion chromatography was carried out under isocratic conditions on a Superose 6 5/150 or Superdex 200 5/150 (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the supernatant was loaded onto 5 mL Nickel Sepharose 6 fast flow resins (GE Healthcare) in a gravity flow column ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared lysate was incubated with 5 ml IgG Sepharose 6 Fast Flow (GE Healthcare) for 3 h ...
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bioRxiv - Immunology 2022Quote: ... The protein was fractionated by size-exclusion chromatography (Superose 6 increase 5/150, GE Healthcare) with buffer F (20 mM HEPES at pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... and eluted from a Superose 6 Increase 5/150GL size exclusion column (GE Healthcare, UK) in 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
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bioRxiv - Biophysics 2021Quote: ... and loaded at 4 °C with a flow rate of 0.5 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Immunology 2023Quote: ... A 5 ml protein G Sepharose 4 Fast flow column (GE Healthcare) was used to extract total human IgG ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was then cleared by centrifugation (18000 g, 4 °C, 1 hour) and purified using a HisTrap HP 5 ml column (GE Healthcare) and a 10-100 mM imidazole linear gradient followed by elution with 250 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: Analytical size-exclusion chromatography (SEC) was performed on a Superose 6 5/150 column (GE Healthcare) in SEC buffer containing 10 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow, 5 mL, GE Healthcare). Samples were eluted with imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Biophysics 2024Quote: ... The clarified lysate was loaded onto a 5 mL nickel-nitrilotriacetic acid (Ni-NTA) resin (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...