Labshake search
Citations for GE Life Sciences :
4601 - 4650 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
-
bioRxiv - Immunology 2021Quote: ... 0.5-1 mL plasma or serum was heat inactivated at 56°C for 45 minutes and incubated overnight with Protein G Sepharose 4 Fast Flow beads (GE Healthcare) at 4°C ...
-
bioRxiv - Immunology 2020Quote: The GST-rNLRP1FIIND S969A-rDPP9 complex and rNLRP1FIIND proteins purified as described above were subjected to gel filtration (Superose 6, 10/30; GE Healthcare) in buffer containing 10 mM Tris pH 8.0 and 100 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
-
bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
-
bioRxiv - Immunology 2021Quote: ... Media was harvested 4 days after transfection and the secreted protein purified using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2021Quote: Samples of purified proteins were thawed on ice and the buffer was replaced using G-25 columns (PD MiniTrap G-25, GE Healthcare) with 25 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... Bound protein was eluted with elution buffer (lysis buffer containing 250 mM imidazole) and loaded onto glutathione Sepharose resin (GE Healthcare) to separate bound NEC from excess His6-SUMO-UL31 ...
-
bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
-
Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... for 2h at 4 °C in Tris/NaCl buffer (50 mM Tris/300 mM NaCl and the tagged protein was purified over a HisTrap FF column (GE Healthcare). GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore ...
-
bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
-
bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used to incubate the membrane for an hour followed by protein detection with enhanced chemiluminescence (ECL) western blotting substrate and viewed on Amersham Imager 600 (GE Healthcare). Uncropped immunoblot images are shown in Figure S8.
-
bioRxiv - Cell Biology 2021Quote: ... The Trx-6xHis-3C tag was removed by protease 3C cleavage and the untagged protein was further purified by size-exclusion chromatography (Superdex 75, GE Healthcare).
-
bioRxiv - Genomics 2021Quote: ... we used the Ascaris proteins cleaved from GST-fusions bound to Glutathione Sepharose 4B by treatment with PreScission protease (GE Healthcare). AsWAGO-2 peptides (YASRRMFELTKQSRDDYRA and RNGGQETSSSSSGEAGHLDI ...
-
bioRxiv - Cell Biology 2021Quote: ... was applied and immunoreactive proteins were visualized either using ImageQuant LAS 4000 machine (Raytek) or chemiluminescence film (Scientific Laboratory Supplies or GE Healthcare).
-
bioRxiv - Cell Biology 2021Quote: ... Image stacks were deconvolved and protein levels at centrioles were quantified based on maximal intensity projections using Softworx Explorer (GE Healthcare). To quantify pericentriolar protein we applied with Imaris (Bitplane ...
-
bioRxiv - Genetics 2020Quote: ... The protein sample was finally purified on size-exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare), pre-equilibrated with buffer (20 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... His6-tagged SECTO-5GS-1TD0 spike protein was extracted from the supernatants using an immobilized Ni SepharoseTM Excel column (GE Healthcare) and eluted with 500 mM Imidazole prior to SEC ...
-
bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2022Quote: ... Both the 6His- and Strep-tagged proteins were then further purified by SEC on an ÄKTA Purifier using a Superdex 200 16/600 GL column (GE Healthcare), and simultaneously buffer-exchanged into 50 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap FF crude 5 ml Ni-based column using ÄKTA Xpress System (Cytiva, former GE Healthcare). For that a wash buffer (50 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
-
bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
-
bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The cleaved TPR-CHAT protein was eluted from GST beads and further fractionated by a Resource Q (AKTA Pure, GE Healthcare) in buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... all proteins were then further purified by size exclusion chromatography using a Superdex 75 10/300 GL increase column (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... Fractions containing the proteins were pooled and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
-
bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
-
bioRxiv - Genomics 2022Quote: ... The supernatant was harvested and the protein was purified by FLAG-affinity purification followed by MonoQ anion exchange chromatography (GE Healthcare). Finally ...
-
bioRxiv - Plant Biology 2022Quote: The purified protein from untagged OscobB was further analysed by a size exclusion chromatography (SEC) passing 0.5mg protein through the SUPERDEX 200 (10/300 GL) column (GE Healthcare, USA) attached to AKTA pure 25 at the rate of 1ml/min ...
-
bioRxiv - Microbiology 2022Quote: ... Eluates were concentrated by centrifugal filtration and proteins were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare) with running buffer PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... The concentrated protein is dialysed against buffer A which was further subjected to purification using the gel-filtration sepharose column (GE healthcare) and collected using an automated AKTA collector ...
-
bioRxiv - Microbiology 2022Quote: ... Fractions containing purified MglA-His6 or His6-MglB proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer A without imidazole for use in GTPase assays or buffer C (20mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins (40 µg) were run on 10% SDS–PAGE gel and transferred to an Amersham Hybond-ECL nitrocellulose membrane (GE healthcare), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The 6xHis-tagged protein was purified from the insoluble fraction of a separately induced culture using Ni-NTA beads (GE Healthcare) with 250 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... A total of 100 μl containing 10 mg/ml of disassembled protein were loaded onto a Superdex 75 Increase 10/300 GL (GE Healthcare) using 300 mM NaCl for elution ...
-
bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... Separation of cleavage tags and aggregated proteins was performed using size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in 20□mM citric acid pH 5.0 ...
-
bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
-
bioRxiv - Neuroscience 2023Quote: ... Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare) eluting in PBS with 1 mM DTT ...
-
bioRxiv - Plant Biology 2022Quote: ... and the supernatant was filtered on 0.45 µm units before loading onto a nickel-Sepharose Fast Flow column (1mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The PLP protein was eluted at 160 mM imidazole ...
-
bioRxiv - Microbiology 2023Quote: 4 mg of native protein standard was loaded to calibrate the gel filtration column (Superdex 200 increase 10/300 GL column, GE Healthcare). Following this ...
-
bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... The PfPHB1-and PfPHB2-GST fusion proteins were purified via affinity chromatography from bacterial extracts using glutathione-sepharose (GE Healthcare, USA) using the soluble fractions of the lysed bacterial cultures ...
-
bioRxiv - Molecular Biology 2022Quote: ... We eluted bound proteins using an imidazole gradient and subjected them to size-exclusion chromatography (Superdex 200 16/600, GE Healthcare) using 20 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant recovered after centrifugation was used to purify proteins with GE HisTrap HP column (GE Healthcare Bio-Sciences, Piscataway, NJ) and a Bio-Rad EconoPump (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... The sample was centrifuged after incubation with TEV and the supernatant containing the cleaved protein was purified by anion exchange chromatography using a column packed with either Q Sepharose High Performance (GE Healthcare) for Pex13 and 14 IDRs or SP Sepharose High Performance (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... Peak fractions containing the protein complexes were pooled and further purified by size exclusion chromatography using a 16/60 Superdex 200 column (GE Healthcare) equilibrated with a buffer containing 200 mM NaCl ...