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Citations for GE Life Sciences :
4551 - 4600 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and a 1 ml HiTrap Q column (GE Healthcare), both equilibrated in a buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.
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bioRxiv - Physiology 2021Quote: ... followed by biotinylated antibody (GE Healthcare, RPN1004V, 1:200), HRP-labeled streptavidin (Southern Biotech ...
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bioRxiv - Biophysics 2020Quote: ... through a 1 mL HisTrap HP column (GE Healthcare) and eluting with 350 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... and sheep anti-mouse IgG (GE Healthcare, 1:2000) HRP-conjugated secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit IgG HRP (1:5000 GE Healthcare NA934V), anti-mouse IgG HRP (1:5000 Abcam ab97046).
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bioRxiv - Cancer Biology 2022Quote: ... and anti-mouse IgG (1:10,000; NXA931V; GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... and concentrated to 1 mL (Vivaspin 20, GE Healthcare). Desalted RSA-sulfo-MBS was added to the peptides ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT) on an ÄKTAmicro system (GE Healthcare). Fractions were analyzed by SDS-PAGE on 4–12 % Bis-Tris gels (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... or sheep anti-mouse IgG (GE Healthcare, 1:10,000) in TBS-T containing 5% milk for 2 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... using anti-Rabbit HRP-conjugated (1:5000, GE Healthcare) as a secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... or 1 mL Ni-NTA HisTrap column (GE Healthcare, for fast performance liquid chromatography (FPLC)-based purification) ...
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bioRxiv - Physiology 2024Quote: ... and sheep anti-mouse (GE Healthcare #NA931V, 1:1000). Protein bands were detected using Chemiluminescent Substrate (Thermo Fischer ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were performed using Dharmafect-1 (GE Healthcare) and 80nM of indicated siRNA ...
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bioRxiv - Biochemistry 2023Quote: ... or donkey anti-rabbit (1:10,000; GE Healthcare #NA934V).
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bioRxiv - Cell Biology 2022Quote: ... or anti-rabbit (GE Healthcare # NA9340, dilution 1:10,000) origin and ECL substrate (Thermo Scientific # 32106) ...
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bioRxiv - Microbiology 2023Quote: ... and a HisTrap FF Crude 1 mL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... loaded on a 1 ml heparin column (GE Healthcare) and eluted in the heparin buffer supplemented with 500 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... using a 1 ml HisTrap-HP column (GE Healthcare), then by a Heparin-affinity purification using a 1 ml HiTrap Heparin-HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... and HiTrap SP XL 1 mL columns (GE Healthcare). Fractions containing DprA were concentrated on an Amicon Ultra-4 centrifugal filter (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary HRP-conjugated antibody was added for 1 hour at a dilution of 1:5000 followed by detection with ECL Prime Western Blotting Detection Reagent (GE Life Sciences; RPN2232) and exposure to autoradiography film at various time intervals or by digital imaging (LI-COR Odyssey) ...
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bioRxiv - Developmental Biology 2021Quote: ... horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844, Amersham, UK) following incubation with ECL for 1 minute (RPN2232, GE Healthcare, IL, USA) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... were freshly isolated from blood collected in EDTA-coated vials by layering diluted blood (1:1 in PBS) on top of an equal volume of Ficoll (GE Healthcare Life Sciences), followed by centrifugation and isolation of the buffy coat.
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...
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bioRxiv - Biochemistry 2020Quote: The human Cdc42 1-179aa Q61L (referred to as Cdc42)-encoding DNA was cloned into a modified pGEX-4T-1 vector (GE Healthcare, Chicago, Illinois) as previously described (Barthelmes et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were desalted using 500 µg of a 1:1 mixture of hydrophobic and hydrophilic Sera-Mag Carboxylate-modified Speed Beads (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Synthetic Biology 2021Quote: ... peripheral blood mononuclear cells were isolated by Ficoll-Paque (GE Healthcare) centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-16-cell embryos were transferred to 3 % Ficoll (GE Healthcare) in 0.6x MMR and injected with 5-10 nl of a solution containing mRNAs and/or MOs ...
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bioRxiv - Immunology 2020Quote: ... Cell culture medium containing 20 % FBS (SH30066.03, HyClone, GE Life Sciences), 10 mM HEPES (15630-080 ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell suspension was loaded onto a discontinuous Percoll (GE Healthcare) gradient (20% ...
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bioRxiv - Molecular Biology 2020Quote: ... Images were acquired using an IN Cell Analyzer 2200 (GE Healthcare), and quantitative image analyses were run in the open-source software CellProfiler (www.cellprofiler.org ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with the indicated siRNAs using DharmaFECT (GE healthcare), HiPerFect (Qiagen ...
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bioRxiv - Immunology 2019Quote: Splenic mononuclear cells were isolated by Ficoll-paque Plus (GE Healthcare) (Little Chalfont ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell were washed with phosphate-buffered saline (PBS, GE Life Sciences), scrape harvested ...
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bioRxiv - Microbiology 2020Quote: ... Individual infected cells were quantified using ImageQuant TL software (GE Healthcare). All pseudovirus work was performed in a Class II Biosafety Cabinet under BSL-2 conditions at Erasmus Medical Center.
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bioRxiv - Cancer Biology 2020Quote: ... plates were analysed using the IN Cell analyser 2000 (GE Healthcare) high-content analysis system ...
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bioRxiv - Cell Biology 2020Quote: ... T151-cells were cultured with tetracycline-free FBS (GE Healthcare, SH30070.03T)22 ...
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bioRxiv - Cell Biology 2022Quote: Human peripheral blood mononuclear cells were isolated by Ficoll (GE Healthcare) density gradient centrifugation and then stained with PE anti-CD3 (1:100 ...
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... Image processing was performed with IN Cell Investigator software (GE Healthcare), applying the provided granularity algorithm ...
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bioRxiv - Microbiology 2022Quote: ... The cell lysate was incubated with Ni-NTA-agarose (GE Healthcare) affinity resin for overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Cells were imaged using a DeltaVision system (Applied Precision, Issaquah, WA) equipped with an inverted Olympus IX70 microscope (60X oil objective ...