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Citations for GE Life Sciences :
4451 - 4500 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were first purified with affinity chromatography using AKTA pure 25 system and HiTrap MabSelect SuRe protein A column (GE healthcare, Cat# 11-0034-94) for huACE2-IgHu-pVax ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with developing solution kit (Amersham™ ECL Select™ Western Blotting Detection Reagent) and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was further purified by size exclusion chromatography using HiLoad® 16/600 Superdex® 75 pg column (GE Life Sciences, 28-9893-33) equilibrated with 1x Tris buffer (50 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The protein samples were then applied to size exclusion chromatography (Superdex 200 prep grad, HiLoad 26/60 or 16/60, GE Healthcare Life Sciences, Freiburg, Germany) using 50 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Plant Biology 2024Quote: ... His-tagged recombinant proteins were overexpressed in Escherichia coli strain Rosetta™ (DE3) and purified via an Äkta chromatography system using HisTrap columns (GE Healthcare, Waukesha, WI, USA). Washing (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: 3D structured illumination microscopy of fixed T cells cells was performed with a commercial Deltavision OMXv4.0 BLAZE microscope (GE Healthcare, Amersham, UK) using a 60x ...
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bioRxiv - Cancer Biology 2020Quote: ... The mantle cell lymphoma cell line Maver-1 (#ACC 717, DSMZ Gmbh, Braunschweig, Germany) was cultured in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin.
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bioRxiv - Cancer Biology 2023Quote: ... which was determined by imaging of DAPI stained cells using the Incell 2200 or 6500 Cell Imaging Systems (GE Healthcare, Parramatta, Australia).
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bioRxiv - Systems Biology 2020Quote: ... Cells were imaged using a DeltaVisionQR Elite (GE Healthcare), with a 60× 1.42NA Oil Plan APO and an InsightSSI 7 Colour Combined Unit illumination system (CFP = 438nm ...
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bioRxiv - Cell Biology 2020Quote: ... cells were reverse-transfected using DharmaFECT 1 (GE Healthcare) and then transfected again 24 h later according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and mononuclear cell isolation on Ficoll-Paque (GE Healthcare) density gradient ...
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bioRxiv - Immunology 2021Quote: ... Cell pellets were resuspended in 37% Percoll (GE Healthcare) and centrifuged at 1200 x g for 30 min with deceleration set to 0 ...
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bioRxiv - Genomics 2019Quote: ... HEK293T cells were purchased from GE Life Sciences (HCL4517). K562 cells were purchased from Sigma-Aldrich (89121407) ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were imaged on a DeltaVision (Applied Precision) imaging system equipped with an IX71 microscope (Olympus) ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were isolated using Ficoll-Paque (GE Healthcare) and stored at −80°C.
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bioRxiv - Cell Biology 2021Quote: ... Cells were visualized using a DeltaVision microscope (Applied Precision) fitted with a 60X ...
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bioRxiv - Neuroscience 2019Quote: The GE IN Cell Analyzer 2500HS (GE Life Sciences) was used for imaging ...
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bioRxiv - Immunology 2021Quote: ... The cells were suspended in 40% Percoll (GE Healthcare). The suspension was overlaid on 70% Percoll and centrifuged at 836 g for 30 minutes at room temperature ...
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bioRxiv - Systems Biology 2021Quote: HEK 293 cells were cultivated in DMEM (GE Healthcare), supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... widefield high content imager (IN Cell 2200, GE Healthcare) using a 10x/0.45 NA lens and standard DAPI (nuclear stain ...
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bioRxiv - Microbiology 2022Quote: ... were taken using IN Cell Analyzer 2000 (GE Healthcare). HepG2 nuclei as well intracellular and extracellular sporozoites were counted using the IN Cell Developer Toolbox v.1.9.2 software (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were first reverse-transfected using DharmaFECT (GE Healthcare) and then transfected again with HiPerFect (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... cells were re-suspended in 37% Percoll (GE Healthcare) and centrifuged at 500g for 30min at 18°C ...
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bioRxiv - Immunology 2019Quote: ... Dead cells were removed by Ficoll centrifugation (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in 44% Percoll (GE Healthcare) on an underlay of 67% Percoll ...
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bioRxiv - Microbiology 2021Quote: ... G1/swarmer cells were isolated using Percoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended into a 30% Percoll (GE Healthcare) solution and centrifuged to separate the fat from the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated cells were resuspended in 40% percoll (GE Healthcare), overlaid onto 80% percoll and centrifuged at 500g (with brake off ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged on a DeltaVision microscope (Applied Precision) using 100X oil immersion lens ...
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bioRxiv - Neuroscience 2024Quote: ... Pelleted cells were resuspended into 30% Percoll (GE Healthcare) and centrifuged at 700 × g for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... COS-7 cells were cultured in DMEM (GE Healthcare) supplemented with FCS (PAN-Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... mononuclear cells (BMMCs) were isolated by Ficoll (GE Healthcare) density gradient centrifugation and stored in liquid nitrogen until further use ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were incubated with Streptactin II (GE healthcare) affinity beads for 2 hours at 4°C while rotating ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were imaged with InCell Analyzer 6000 (GE Healthcare). Eight fields were captured from 2 wells ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-rabbit IgG (Cell signaling, GE Healthcare) used in a dilution of 1:10000 and incubated at room temperature for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... pGEX-4T-1 cells were obtained from GE Healthcare. All other reagents and chemicals used were of the highest grade available.
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bioRxiv - Immunology 2024Quote: ... The apoptotic cells were stained with CypHer5 (GE Healthcare) and seeded together with normal and metastasis-associated macrophages isolated from Clec4f tdT-Cre;CD68EGFP mice or H11DreERT2;Clec4f tdT-Cre;R26LSL-RSR-tdT-DTR;Ccr2GFP/GFPmice in a non-tissue culture-treated plate at 1:10 phagocyte to cancer cell ratio for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biochemistry 2021Quote: Size exclusion chromatography (SEC) of the concentrated protein solution was conducted using a HiLoad™ 26/600 Superdex™ 200 pg column (GE Healthcare, Mu-nich, Germany) coupled to the aforementioned chromatography system ...
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bioRxiv - Cancer Biology 2019Quote: ... The immune complexes were mixed with 20 μl of Protein A or G sepharose 4 Fast flow beads (50% v/v, GE Healthcare Life Sciences, Marlborough, MA, USA). Whole cell lysates and immunoprecipitates were detected by Western blotting ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...