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Citations for GE Life Sciences :
401 - 450 of 661 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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bioRxiv - Microbiology 2020Quote: ... The PhuS or PhuS H212R mutant lysate was applied to a Sepharose-G column (GE Life Sciences) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 5 ml GSH column (GE Healthcare) equilibrated in washing buffer (lysis buffer without DNAse and PMSF) ...
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bioRxiv - Cell Biology 2019Quote: ... 250 μL of cleared lysate was fractionated on Superose6 Increase 10/30 gel filtration column (GE Healthcare) equilibrated in running buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was passed over a self-packed Ni2+-charged IMAC Sepharose 6 Fast Flow (GE Healthcare) column ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences). To slow Fe2+-oxidation and precipitation in equilibration buffer ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare) equilibrated in Buffer A (50 mM Hepes pH8 ...
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bioRxiv - Cell Biology 2022Quote: ... The cleared lysate was subsequently loaded onto a pre-equilibrated HisTrap column (GE Healthcare 1mL HisTrap column) using an ÄKTA Pure Protein Purification System (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protan, GE Healthcare). Incubation with specific antibodies was followed by peroxidase-conjugated secondary antibodies and detection using SignalFire ECL Reagent (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was loaded onto a 1 ml StrepTrap HP column (GE Healthcare Life Sciences, Buckinghamshire, UK) that was equilibrated with Lysis buffer.
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bioRxiv - Developmental Biology 2023Quote: ... lysates were shaken with a 1:1 mixture of hydrophilic and hydrophobic Sera-Mag SpeedBeads (GE Healthcare) in a final concentration of 50% ethanol for 8 mins at 23 ℃ ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysate was applied to a nickel affinity column (GE Healthcare; Sepharose high performance beads) equilibrated in Buffer A and purified by FPLC ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were incubated with 70⍰μl of streptavidin beads (5⍰ml; 17-5113-01, GE Healthcare) at 4° for 3⍰h ...
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysates were added to 1.5 mL glutathione-Sepharose 4B resin (17-0756-05, GE Healthcare) that had been pre-washed with buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was clarified by centrifugation (65,000 rpm for 45 min) and incubated with IgG Sepharose (GE Healthcare) for 2 h at 12 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The cleared lysate was incubated with 0.5 mL of a nickel sepharose resin (GE healthcare, Little Chalfont, UK), pre-equilibrated with 50 mM of KH2PO4 buffer (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... and equal amounts of lysates were filtered through a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) and washed with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... the cell lysates were directly subjected to size exclusion chromatography using an ÄKTA purifier (GE Healthcare Life Science). The column was washed with three column volumes (75 ml ...
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bioRxiv - Microbiology 2020Quote: ... All protein lysates were separated on 12% SDS-polyacrylamide gels and transferred to polyvinyl difluoride (PVDF, GE Healthcare) membranes ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate supernatant was than loaded on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) pre-equilibrated with a binding buffer (8 M Urea ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and His6-KIAA1841(BTB) was isolated on a HisTrap FF column (GE Healthcare). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant from the centrifuged cell lysate was loaded onto a Q Sepharose fast flow column (GE Healthcare). In vitro nitrogenase activity was determined by monitoring acetylene reduction to ethylene as previously described.14 Ethylene and acetylene were quantified using gas chromatography (activated alumina 60/90 mesh column ...
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bioRxiv - Biophysics 2022Quote: ... Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare). Then the proteins were biotinylated using Avidity biotinylation kit and recommended protocol ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were injected (200 µL per injection) onto a Superose® 6 10/300GL column (GE Healthcare, UK) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Filtered lysate was loaded onto a pre-equilibrated 1 mL or 5 mL HisTrap HP column (GE Healthcare) charged with Ni2+ ...
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bioRxiv - Microbiology 2021Quote: ... Enzymes were isolated from the cleared lysates via nickel affinity chromatography (IMAC) using a HisTrap column (GE Healthcare) and size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was divided equally and incubated with 50 μl of prewashed 50% Protein G slurry (GE Healthcare) for each condition ...
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bioRxiv - Molecular Biology 2022Quote: ... The cleared lysate was then injected into the equilibrated column connected to ÄKTA pure purification system (GE Healthcare). We did not observed the clogging of the column and the system ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate was passed through a HiTrap SP HP cation exchange chromatography column (GE Healthcare Life Sciences) to remove impurities ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl) and the lysate was batch binding with 300 μL Ni-NTA column (GE Healthcare, USA). After an extensive washing with buffer A ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto a HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...