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Citations for GE Life Sciences :
401 - 450 of 2537 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of protein was injected onto a Superdex 75 increase 10/300 GL column (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... completing with up to 100% imidazole solution in 15 minutes using an ÄKTA FPLC system (GE Healthcare). Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
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bioRxiv - Biophysics 2021Quote: ... The dual-labeled heterodimer was then purified by cation exchange chromatography (Mono S 10/100, GE Healthcare) in 25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then applied to a HiPrep 26/60 Sephacryl S-100 column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.0 and 5 mM β-mercaptoethanol) containing 100 mM NaCl by using desalting column (GE Healthcare) and further purified by affinity chromatography with HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by gel filtration (HiLoad 16/60 Superdex 75 or HiLoad 16/60 Superdex 100, GE Healthcare) as previously described [38] ...
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bioRxiv - Cell Biology 2020Quote: ... 100 mM NaCl and 0.5 mM TCEP) and re-loaded onto the HisTrap HP column (GE Healthcare) to remove the tag ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Cell Biology 2019Quote: ... actin in the supernatant was loaded into HiPrep 16/60 Sephacryl S-100 HR (GE healthcare, #17116501) pre-equilibrated with G-buffer for a size-exclusion chromatography (SEC) ...
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bioRxiv - Immunology 2021Quote: ... debris and aggregates were removed using a 100/60/40/30-percent gradient of Percoll (GE HealthCare) in RPMI 1640 (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... was followed by molecular exclusion with the column HiPrep 26/60 Sephacryl S-100 HR (GE HealthCare), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA (100 ng) was dephosphorylated with calf intestine alkaline phosphatase (GE Healthcare Europe GmbH, Glattbrugg, Switzerland), denaturated with dimethyl sulfoxide ...
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bioRxiv - Microbiology 2023Quote: ... The pull-down of biotinylated proteins was performed using 100 µl StrAv Mag Sepharose™ (GE Healthcare) for each condition and incubated in a rotation wheel for 3h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the crude mixture was purified using a MonoQ HR 10/100 GL anion exchange column (GE Healthcare) with the following gradient system ...
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bioRxiv - Biophysics 2023Quote: ... The sample was further purified by anion-exchange chromatography on Mono Q 10/100 GL (GE Healthcare; 160 ml linear gradient of 300-500 mM NaCl in 10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... 100-250 mM NaCl and 2 mM DTT using the ÄKTA™ Pure 25 system (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... the crude mixture was purified using a MonoQ HR 10/100 GL anion exchange column (GE Healthcare) with the following gradient system ...
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bioRxiv - Microbiology 2024Quote: ... One milliliter of the supernatant was then incubated with 100 µL Glutathione Sepharose (GS) resin (GE Healthcare), pre-equilibrated in buffer F for 1 hour at 4°C with constant agitation at 1,000 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1 mL with buffer B and added immediately onto an anion exchange resin (Q resin; GE Healthcare; 0.2 mL; equilibrated in buffer H) and incubated (5 min) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Bioengineering 2023Quote: ... heated to 60°C to remove aggregates and further purified on two coupled IEX columns 1 mL HiTrap Q FF and 1 mL HiTrap SP FF (GE Healthcare Life Sciences, NSW, Australia) by collecting the flow-through ...
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Biochemistry 2021Quote: ... loaded onto a 5-ml HiTrap Heparin HP column (GE Healthcare), and eluted with a 12-CV gradient from buffer A1 to A2 (1.5 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was loaded onto a 5 ml HisTrap column (GE Healthcare) pre-loaded with Ni2+ ...
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bioRxiv - Biochemistry 2020Quote: ... eluted though a tandem 5 ml HiTrap desalting column (GE Healthcare) to remove excess cross-linking reagents ...
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bioRxiv - Immunology 2021Quote: ... 20 ml of EAH Sepharose 4B resins (GE Healthcare Life Sciences) were washed with distilled water three times and mixed with 100 mg of DS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Biochemistry 2020Quote: ... at 0.5 ml/min using an ÄKTA™ Pure (GE Healthcare). The column outlet was fed into a DAWN® HELEOS™ II MALS detector (Wyatt Technology) ...
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bioRxiv - Biochemistry 2020Quote: ... at 0.5 ml/min using an ÄKTA™ Pure (GE Healthcare). Elution fractions of wild-type and mutant proteins for each KASH binding-partner were analysed by SDS-PAGE.
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bioRxiv - Biochemistry 2020Quote: ... protein was loaded onto a 5 mL HisTrap column (GE Healthcare) and eluted with a linear imidazole gradient (20-350 mM ...
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bioRxiv - Biochemistry 2020Quote: ... and applied onto a GE HisTrap HP 5 mL (GE Healthcare). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... at 0.5 ml/min using an ÄKTA™ Pure (GE Healthcare). The column outlet was fed into a DAWN® HELEOS™ II MALS detector (Wyatt Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... and applied to a 1 mL HisTrap HP column (GE Healthcare) using an AKAT Prime Plus (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified using 5 mL Ni-NTA column (GE Healthcare) and a HiLoad Superdex200 16/60 size exclusion column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... A 1 ml HisTrap FF Crude chromatography column (GE Healthcare, USA) was equilibrated with 5 ml of buffer (30 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was loaded to 5 mL HisTrap HP column (GE Healthcare). With rPtxS1 proteins ...
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bioRxiv - Biophysics 2020Quote: ... and desalted (5 ml HiTrap, GE Healthcare Life Science, Chicago, USA). KR2 was incorporated into liposomes with an inside-out orientation ...
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bioRxiv - Biophysics 2020Quote: ... were purified using 5 ml SPFF cation exchange column (GE Healthcare) with a 100 ml gradient of 0.1 to 1 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Biochemistry 2020Quote: ... and circulated through a 5 ml HisTrap HP column (GE Healthcare) for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... equipped with 1-ml HisTrap affinity column (HisTrap HP, GE Healthcare) loaded with Ni (II ...
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bioRxiv - Microbiology 2020Quote: ... the cells were resuspended in one mL R10 (RPMI [GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2019Quote: ... 1.5 mL of collagenase buffer (10mL DMEM (GE Life Sciences, SH30081.01), 0.20 g Collagenase Type 2 (Worthington Biochemical ...
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bioRxiv - Bioengineering 2021Quote: ... and loaded into a HisTrap HP 5 mL column (GE Healthcare) using an ÄKTA pure 25 (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... and on one 5 ml StrepTrap HP column (GE Healthcare, US) equilibrated in binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...