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Citations for GE Life Sciences :
401 - 450 of 1392 citations for Rat Wide range C Peptide CP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: The human PCM1 C-terminal fragment was cloned into pGEX-2TK GST fusion vector (GE Healthcare Life Sciences) using the primers ...
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bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Time-lapse imaging was carried out at 27 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision) comprising an Olympus IX70 widefield inverted epifluorescence microscope ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Genomics 2019Quote: ... hybridization (at 68°C in 50% formamide) and washing was done following the instructions of the manufacturer (GE Healthcare). The riboprobes were obtained by rub-off transcriptions of linearized pBlueScript plasmids containing the full retrozymes in the presence of DIG-UTP (Roche Diagnostic GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... dried for one hour at 80°C and exposed overnight to Phosphor screen in a cassette (GE healthcare, USA). Images were captured in Fujifilm FLA 5100 scanner (Japan ...
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bioRxiv - Biochemistry 2020Quote: ... Analytical size exclusion chromatography was performed at 24 °C using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a mobile phase containing 10 mM HEPES (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: ... Two 2 ml aliquots were collected using GF/C glass microfiber filters (20 mm; Whatman, GE Healthcare Life Sciences), transferred to glass tubes ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Cell Biology 2022Quote: Living embryos were imaged at 21°C using a DeltaVision OMX V3 Blaze microscope (GE Healthcare Life Sciences, UK). The system was equipped with a 60x/1.42 NA oil UPlanSApo objective (Olympus Corp.) ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was then incubated with increasing amounts of ribosomal subunits/ribosomes for 15 min at 37°C before being rapidly filtrated through a cellulose nitrate membrane filter (0.2μm, GE Healthcare). The cellulose nitrate membranes were washed with 1 mL of cold TAKM5 buffer and placed into 10 mL of EcoLite (+ ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Immunology 2020Quote: ... culture media was collected and incubated overnight at 4°C with nickel-Sepharose Excel (GE Healthcare 17-3712-01). His-tagged proteins were eluted from the nickel-Sepharose with 1 M imidazole in TrisCl pH 8.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 1 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2022Quote: ... incubated at 4 °C for 2 hour and further purified by Superose 6 increase 10/300 column (GE Healthcare). The peak tube was concentrated to 0.4-0.5 mg/mL in 20 mM Tris pH8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted mononucleosome fraction was incubated for 2 h at 4°C with Protein G Sepharose beads (GE Healthcare), which were preincubated with rabbit polyclonal anti–chicken CENP-A antibody64 ...
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bioRxiv - Plant Biology 2024Quote: Analytical SEC experiments were carried out at 4 °C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in SEC running buffer ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining purification steps were carried out at 4 °C using an ÄKTA protein purification system (GE Healthcare/Cytiva) for column chromatography steps ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Bioengineering 2024Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrapTM HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Microbiology 2024Quote: ... The complex was resolved by gel filtration at 4 °C with a Superdex 200 10/300 column (GE Healthcare) equilibrated with 75 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were dried under vacuum at 75°C onto 3MM paper (Whatman) and imaged using Typhoon phosphorimager (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... ECL chemiluminescent kit (RPN2132, GE Healthcare) was used for detection according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The ECL Prime Kit (GE Healthcare) was used for HRP detection.
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bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Bioengineering 2020Quote: ... 10% glycerol (v/v) at 4°C and re-loaded onto a Ni-NTA column (HisTrap™ HP, GE Healthcare). An imidazole gradient was applied (from 20mM to 500mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was recovered by centrifugation (50,000 g, 20 min, 4°C) and applied to a HisTrap FF NiNTA column (GE Healthcare). After washing with buffer A (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Titrations were performed at 25°C with a stirring speed of 750 rpm using the MicroCalTM iTC200 System (GE Healthcare). A total of 20 injections with 2 μL each and a spacing of 180 seconds were titrated into the 200 μL protein sample (25 mM Hepes pH 7.5 ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The complex was incubated for 10 min at 22°C before size exclusion chromatography using a Superdex 200 Increase 3.2/300 column (GE Healthcare) at 22°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was pre-equilibrated for 1.5 hrs at 4°C with Sup6-150 buffer without inhibitors or DTT on an AKTA Purifier system (GE Healthcare). 500 μL lysate (2 mg protein ...
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bioRxiv - Biochemistry 2019Quote: ... Following dialysis the E215C K490 construct was reacted for 4 h at 4°C with Cy3-maleimide (GE Healthcare, PA13131) at a motor/Cy3 dye ratio of 1:10 as previously described (Tomishige et al ...