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Citations for GE Life Sciences :
401 - 450 of 559 citations for Prestained SDS PAGE Standards Single Color since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: RNA was separated on 12 % Urea-PAGE in 0.5x TBE and transferred to Nylon membranes (Roche, GE Healthcare) using semi-dry blotting in 0.5x TBE for 30 minutes at 5 V=constant ...
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bioRxiv - Neuroscience 2020Quote: ... the stimulation was quenched by adding hot lysis buffer (final concentrations: 0.1% SDS (GE Healthcare), 25 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: Proteins were resolved on 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare). The primary antibodies used were mouse anti-EHD2 (L-05 ...
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bioRxiv - Cell Biology 2020Quote: ... High molecular weight gel filtration standards (Thyroglobulin, Aldolase and Ovalbumin) were purchased from GE Healthcare (Catalog #28403842) and used to estimate the Stokes’ radii of eluted proteins in the same buffer as used for corresponding SEC run69.
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bioRxiv - Bioengineering 2020Quote: The images were reconstructed using the manufacturers’ standard algorithms (Toshiba reconstruction filter AIDR3D, FC08-cfa, GE Healthcare AsirV=0.5 standard kernel ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Biochemistry 2021Quote: ... All columns were calibrated using protein standards from both high and low molecular weight kits (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... blots were processed for chemiluminescence using standard protocols (ECL Prime Western Blotting Detection Regent #RPN2236, GE Healthcare), and signals were detected using a LAS 4000 Imager (Fuji Film).
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bioRxiv - Cell Biology 2021Quote: ... labelled probe was purified by native TBE-PAGE gel and illustra G-25 columns (GE Healthcare, Cat. No. 27532501). Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif ...
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bioRxiv - Biochemistry 2022Quote: ... The substrates and products were resolved by 15% urea-denaturing PAGE gel and quantified with Typhoon (Amersham, GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... The PAGE gel was exposed to a phosphorimage screen and analyzed using Typhoon phosphorimager and ImageQuant software (GE Healthcare). Band intensities were analyzed by creating line profiles of each lane using ImageQuant ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 8-15% SDS gels and transferred to a nitrocellulose membrane (GE Healthcare) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... 250 mM glycine and 0.1 % SDS) and transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare). The membrane was blocked with 5 % bovine serum albumin (BSA ...
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bioRxiv - Systems Biology 2023Quote: ... proteins in 2% SDS were bound to paramagnetic beads (SeraMag Speed beads, GE Healthcare,#45152105050250,#651521050502) by addition of ethanol to a final concentration of 50% ...
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bioRxiv - Neuroscience 2019Quote: ... Mononuclear cells were isolated from CB and PB by density centrifugation using standard Ficoll-Hypaque technique (GE Healthcare) then treated with 0.15M NH4Cl to lyse residual erythrocytes and washed in phosphate-buffered saline (PBS).
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare). PBMC were frozen in 10% DMSO/FBS and stored in liquid nitrogen until analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were isolated from heparinized fresh blood by standard density gradient centrifugation with Ficoll-Paque Plus (GE Healthcare). PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201 ...
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bioRxiv - Molecular Biology 2023Quote: Western blotting was performed using standard procedures and blots visualized with Amersham Imager 600 or 800 (GE Healthcare). GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8 ...
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bioRxiv - Immunology 2021Quote: ... single cell suspension of thymi were subjected to density-gradient centrifugation with a three-layer Percoll gradient (GE Healthcare) with gravities of 1.115 ...
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bioRxiv - Cancer Biology 2021Quote: ... The 12 fields were then stitched together into a single image post-acquisition using the GE Developer Toolbox (v1.9.3; GE Healthcare). All plates were imaged at 8-hour intervals for a total of 7 time points ...
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bioRxiv - Biochemistry 2019Quote: ... Complexes were then run using 5% native PAGE in 0.5× TBE and visualized using the Typhoon 9400 variable mode laser scanner (GE Healthcare).
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bioRxiv - Physiology 2019Quote: ... Purity of the isoHb fractions was verified by means of native polyacrylamide gel electrophoresis (PAGE) using a PhastSystem (GE Healthcare) in 10-15% gradient precast gels ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA products were extracted with phenol:chloroform:isoamyl alcohol and analyzed on an 8% urea-PAGE using fluorescence scanner (Typhoon™ FLA7000, GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE and were transferred from a 4-12 % Bis-Tris NuPAGE gel to a 0.45 µM nitrocellulose membrane (GE Healthcare) using an XCell II Blot module (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... SDS-PA gels were blotted onto Amersham Hybond P 0.2 μm PVDF membranes (GE Healthcare, Chicago, Illinois) using an EasyPhor Semi-Dry-Blotter (Biozym Scientific ...
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bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... The Southern was washed with 1XSSC + 0.1% SDS and imaged using a Storm 825 phosphorimager (GE Healthcare) usually overnight and analyzed with ImageQuant software.
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bioRxiv - Biophysics 2020Quote: ... 10 mM SDS) and then purified using size exclusion chromatography (HiLoad 16/600 Superdex 200; GE Healthcare) in buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Microbiology 2019Quote: ... All samples and standards (500 µl total volume) were separated on a Superdex 200 10/300 column (GE Healthcare) attached to an AKTA-format FPLC system using a flow-rate of 0.5 ml/min and PBS as a running buffer ...
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bioRxiv - Microbiology 2021Quote: ... CM5 chip was first coated with streptavidin by standard amine coupling using an amine coupling kit (Biacore, GE Healthcare), followed by immobilization of biotin-heparin (38) ...
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bioRxiv - Biophysics 2019Quote: ... We selected four proteins as molecular mass standards from the gel filtration calibration kit HMW (GE Healthcare, Uppsala, Sweden), i.e ...
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bioRxiv - Biochemistry 2019Quote: ... The size-exclusion column was calibrated using a set of standard proteins (Gel Filtration HMW Calibration Kit, GE Healthcare) in SEC buffer ...
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bioRxiv - Cancer Biology 2019Quote: Cells for single cell RNA-seq were prepared by density centrifugation using Ficoll-Paque media (GE Healthcare, 17-5446-02) followed by magnetic-activated cell sorting (MACS ...
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bioRxiv - Genomics 2020Quote: The total genomic DNA was extracted from a single aphid using Illustra Nucleon Phytopure kit modifying the manufacturer’s protocol (GE Healthcare). The lysis step was performed adding 10 ul of Proteinase K and incubating the sample in a water bath for 2 hours at 55°C ...
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bioRxiv - Bioengineering 2021Quote: ... TILs were then isolated from the single cell suspension using a density gradient with Percoll Centrifugation Media (GE Life Sciences) and DMEM Media (10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted for 7 s at force 10 using single-layer Whatman filter papers (1442-005, GE Healthcare) before plunging into liquid ethane using an FEI MarkIV Vitrobot at 8 °C and 100% humidity ...
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bioRxiv - Biochemistry 2021Quote: Single cysteine mutants of ColE9 or the ColE9 DNase domain were desalted on a 5 ml HiTrap column (GE Healthcare) equilibrated with binding buffer (20 mM potassium phosphate pH 7 ...
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bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were passed through a 100 µm strainer and decidual leukocytes were enriched using a Percoll (GE Healthcare) density gradient ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Molecular Biology 2021Quote: ... The PAGE gel was exposed to a phosphosimage screen for ∼2 hours and analyzed using a Amersham Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Molecular Biology 2019Quote: ... from mouse liver was separated by 10% denaturing polyacrylamide gel electrophoresis (PAGE) and blotted onto a nylon membrane (Amersham Hybond N+; GE Healthcare) using a Transblot Turbo apparatus (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction products were separated by denaturing (8 M urea) 10% PAGE and were visualized by using a Typhoon phosphorimager (GE Healthcare). As described previously,48 band intensities were determined and used to estimate the fraction of RNAs bound to ligand ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were resolved by SDS-PAGE, and in-gel fluorescence (λex=633 nm, λem=670 nm) was detected using a Typhoon FLA 9000 imager (GE Healthcare). The proteins were then transferred to nitrocellulose membranes and analyzed by Western blot ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Molecular Biology 2022Quote: Protein samples were run on 5∼20% precast gradient SDS-PAGE (ATTO) or 4∼20% precast gradient gel (Shanghai WSHT Biotechnology Inc.) and then transferred to Hybond ECL membranes (GE Healthcare) followed by western blot analysis with the indicated antibodies ...