Labshake search
Citations for GE Life Sciences :
401 - 450 of 990 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Physiology 2021Quote: 200 μl of pooled plasma from gender and genotype matched mice (n = 4-9) was loaded onto a Superose 6 column (GE Healthcare) calibrated with elution buffer (0.15 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with an Olympus UPIanApo 100× numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Neuroscience 2020Quote: ... injection of 185 MBq [123I]-2ß-carbometoxy-3ß-[4-iodophenyl]-N-[3-fluoropropyl]nortropane ([123I]FP-CIT, GE Healthcare, Amersham, UK). The SPECT data acquisition was begun after exactly 3 hours ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Cell Biology 2019Quote: ... 2) A Deltavision Elite System (GE Healthcare) equipped with an IX-71 inverted microscope (Olympus) ...
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bioRxiv - Immunology 2019Quote: ... 20U/ml Xuri IL-2 (GE Healthcare) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... The elute from Q Sepharose was loaded on to 0.20 L source 15RPC (GE Healthcare, USA) column to separate non-glycosylated and glycosylated rhEPO ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were washed extensively with TBS-T before chemiluminescent detection using the ECL western Blotting Detection Reagents (GE Healthcare Life Sciences, RPN2106) and X-ray film or VersaDoc system (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Molecular Biology 2019Quote: ... characterization of an N-terminal Aβ peptide DAE-EG (DAEFRHDSGYSGKQKSRNEGKGGC) binding to anti-mouse captured antibodies from hybridoma supernatants was performed using a BIAcore T-200 instrument (GE Healthcare, Marlborough, MA) [36] ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated and depleted of CD8+ T cells in a single step by density centrifugation using FicollPaque PLUS (GE Healthcare Life Sciences), SepMate tubes (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... membranes were washed 3x in PBS-T and then incubated with appropriate secondary antibody conjugated with horseradish peroxidase (GE Healthcare, Chicago, IL, USA). Chemiluminescence was accomplished using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and at the indicated time points (T=4d, 8d, 12d, 16d, 20d) using a 4X objective (InCell 6000 Analyzer, GE Healthcare Life Sciences). Segmentation and counting of GFP- and mCherry-positive cells were performed using an Acapella script (PerkinElmer).
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of DNA from each specimen were spotted on a piece of Nytran N nylon blotting membrane (GE Healthcare Life Sciences). The membrane was incubated with a primary antibody that recognized Pt-induced DNA adducts (ICR4 ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were separated by electrophoresis using a 1% agarose gel and transferred to nylon membranes (Hybond N+®, GE Healthcare), where hybridization was carried out using an 812 bp fragment of Micropia from D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...