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Citations for GE Life Sciences :
401 - 450 of 1343 citations for IL 2R alpha CD25 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... which was transferred to the Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 recombinant proteins were purified over a His-TRAP FF column (GE Healthcare) with 1-mL bed volume ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done using 5 mL pre-packed Ni-sepharose AKTA Hi-TRAP column (GE Healthcare, USA) equilibrated (100 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl of equilibrated in lysis buffer His Mag Sepharose beads Ni (GE28-9799-17, GE Healthcare) was added to each sample and kept on a roller overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the 6x His-MBP fusion was further purified on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Biochemistry 2021Quote: HR1 with C-terminal poly-his tag was immobilized onto a NTA sensor chip (from GE Healthcare) to around 500 RU using a Biacore SPR 8K (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration chromatography with Superdex-75 SEC column (Hi-Load 16/600, Prep Grade, GE Healthcare, USA) and MALDI analysis were used to determine the molecular mass of PrASNase [23].
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Plant Biology 2023Quote: ... Each of the His-tag-fused LssaCA muteins was bound to Ni Sepharose (GE Healthcare, Uppsala, Sweden), then the GST-NP protein or the GST control protein was added to the beads ...
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bioRxiv - Plant Biology 2023Quote: ... GST-SnRK2.6 and His-MBP-SIZ1-C were mixed separately with high-affinity GST resins (GE Healthcare) in a pull-down buffer containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2023Quote: ... was then purified by Ni-NTA using a His-TRAP FF 5-ml column (GE Healthcare®). Collected elutions were dialyzed into 10 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on the His trap HP 5 mL column packed with Nickel (GE Healthcare). FPLC was used for elution of the GST-His6x-TEV-KIX protein with 300 mM imidazole in 50 mM Tris-HCl buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Biophysics 2022Quote: ... crude lysates were purified on a HiTtap TALON column (GE Healthcare; Chicago, IL), cleaved on the column with 3C protease and followed by size exclusion chromatography (HiLoad Superdex 200 ...
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bioRxiv - Neuroscience 2021Quote: ... and then loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: Immunofluorescence images were acquired on Delta Vision Elite (GE Healthcare, Chicago, IL, USA) using a OLYMPUS 20x/0,75 objective ...
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bioRxiv - Immunology 2021Quote: ... C monoclonal antibody (W6/32) coupled Sepharose-4B resin (GE Healthcare, IL, USA) at room temperature for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... The HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare, Chicago, IL) was either equilibrated as for the other proteins or alternatively equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... using a Superdex 75 pg 16/60 column (GE Healthcare, Chicago, IL, USA). The running buffer contained 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Images were captured by using the Delta Vision microscope (GE Healthcare, Chicago, IL). Fluorescence intensity was quantitated from captured images using ImageJ.
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bioRxiv - Cancer Biology 2021Quote: ... Antigen revelation was performed using the ECL kit (GE Healthcare, Chicago, IL, USA) and images were acquired on a MyECL machine (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Horseradish peroxidase-conjugated α-mouse antibody (1:10,000; NA931V, GE Healthcare, IL, USA) was used as a secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... gel filtration column HiLoad 26/600 Superdex 200 pg (GE healthcare, IL, USA) and anion exchange column HiTrap Q HP (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The purified proteins were desalted with desalting column (GE Healthcare, Chicago, IL, USA) using AKTA system (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred to Hybond P 0.45 PVDF membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked with 5% BSA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated anti-rabbit IgG donkey polyclonal antibody (GE Healthcare, Chicago, IL; #NA934V), HRP-conjugated anti-sheep IgG donkey polyclonal antibody (Chemicon International ...
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bioRxiv - Microbiology 2020Quote: ... Gels were placed on a Phosphor Screen (GE Healthcare, Chicago, IL, United States) overnight (16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Real-time B-mode ultrasonography (NextGen LOGIQe R8, GE Healthcare, Chicago, IL, USA) and a multi-frequency linear-array transducer (L4-12T ...
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bioRxiv - Immunology 2019Quote: ... using Superose™ 12 10/300 GL SEC column (GE Healthcare, Chicago, IL). MRI data were acquired on a Bruker ClinScan 7 T MRI (Billerica ...
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bioRxiv - Biochemistry 2019Quote: ... loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare, Chicago, IL) pre-equilibrated with buffer E (HEPES pH 7.0 ...
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bioRxiv - Neuroscience 2020Quote: ... and then visualized using HRP-conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) followed by ECL prime (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then applied to a 5 ml HiTrap column (GE Healthcare, Chicago, IL) that had been equilibrated with phosphate or PBS buffer (depending on the application) ...
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bioRxiv - Microbiology 2020Quote: ITC experiments were performed on a MicroCal Auto-iTC200 (GE Healthcare, Chicago, IL). Prior to the measurement ...
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bioRxiv - Microbiology 2020Quote: ... followed by size exclusion chromatography (Superdex 200 16/90; GE Healthcare, Chicago, IL) and washed with 0.5 M Arginine in Dulbecco’s PBS pH 7.4 (DPBS ...