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Citations for GE Life Sciences :
401 - 450 of 588 citations for Corning Lymphocyte Serum Free Medium since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... All tissue culture media was supplemented with 10% HyClone Fetal Bovine Serum (GE Healthcare Life Sciences, SH3091003) and 1X Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 100μg/ml of streptomycin and 5% fetal calf serum (FCS; HyClone, GE Healthcare, South Logan, UT, U.S.A.) at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Neuroscience 2023Quote: HMC3 cells were grown in EMEM media (ATCC) supplemented with 10% of bovine calf serum (GE healthcare) and 1x of Antibiotic and Antimycotic (Gibco ...
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bioRxiv - Microbiology 2020Quote: 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (GE Healthcare Life Sciences) supplemented with 10% FBS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated medium was purified using a HiTrap protein A column (GE Healthcare) as previously described (41 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary CLL cells were grown in HyClone™ RPMI 1640 medium (GE Healthcare) supplemented with 10% heat-inactivated FBS and 1% Penicillin/Streptomycin at 37°C and 5% CO2.
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bioRxiv - Immunology 2020Quote: ... Cells were frozen down in freezing medium containing FBS (Hyclone, GE Healthcare, SH30071.03) and 10 % dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GE Healthcare), supplemented with 5% fetal bovine serum and penicillin-streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... the medium was filtered through a 0.2 μm filter (GE Healthcare Whatman™) directly to an ultra-clear centrifuge tube (Beckman Coulter®) ...
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bioRxiv - Immunology 2020Quote: ... the harvested medium was incubated with rProtein A Sepharose FF resin (GE healthcare). The resin was then washed with 20 column volumes of PBS and the antibody was eluted with 50 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT-RPE1 and IMCD3 cells were cultured in DMEM/F12 medium (GE Healthcare) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were loaded onto a Sephadex G-25 medium column (GE Healthcare) to exchange the buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in Dulbecco’s modified Eagle’s medium (Hyclone; GE Healthcare Life Sciences, USA) with 0.2% fetal bovine serum ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were dried and radioactivity in bound and free bands were quantified using a Phosphorimager (GE) and ImageQuant 5.2 software (GE Healthcare, IL). Plots of the fraction bound RNA vs ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling of SNAP fusion proteins and depletion of free dye was confirmed by SDS-PAGE followed by fluorescence imaging on a Typhoon imager (GE Healthcare). Extracts were checked to verify transcription activity (e.g. ...
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bioRxiv - Biochemistry 2020Quote: ... The 8 × His tag was removed by TEV protease and tag-free WDR522-334 was further purified by gel filtration using a Superdex 75 (10/300) increase column (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... 10.2 µM protein was incubated with 100 µM probe and 0.5 mM TCEP for ∼20 h at 4 °C and free probe was removed by desalting using a 5 mL HiTrap desalting column (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were then passed through Glutathione Sepharose resin to remove the free GST and concentrated to run through Superdex-75 (GE Healthcare) into 200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Microbiology 2022Quote: ... The free fluorescent probe was removed by a step of gel filtration chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) in the reaction buffer 50mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was quenched with 10 mM DTT and free dye was separated by size exclusion chromatography on a HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore) ...
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bioRxiv - Immunology 2020Quote: ... Conjugated VLPs or NPs were then separated from free HA trimers by SEC on a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and AAV2-CMV-mKO) were produced using a helper-free triple transfection procedure and purified via affinity chromatography (GE Healthcare, USA).
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Broken cells then underwent ultra-centrifugation at 142,000 x g for 60 minutes before collecting the cell free extract and incubating it with pre-equilibrated HisTrap Ni2+ resin (GE Healthcare) in a 3:1 volume ratio for 2 hours under gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through (containing soluble free histones) was filtered with 0.22 μ filters and loaded on a cation exchange Resource S column (GE Healthcare) equilibrated with the dilution buffer EB5_1 (50 mM Tris-HCl pH8) ...
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bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The tag-free protein was further purified by size-exclusion chromatography on a Hiload 16/60 Superdex 75 column (GE Healthcare). Mutants and ZF truncations were expressed and purified using the same procedure as for the wild-type proteins ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were resolved by stain-free sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane (GE Healthcare) using the semi-dry Trans-Blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The His-tag free MUC5AC-D3 variants were loaded onto a HiPrep Q HP 16/10 anion exchange chromatography column (GE Healthcare) and eluted in a 0-500 mM NaCl gradient in 20 mM Tris pH 8.
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bioRxiv - Cell Biology 2024Quote: Cell-free extracts from Drosophila heads were incubated with a 50% slurry of equilibrated Protein A-Sepharose beads (GE Healthcare Ltd) in the ratio of 100 µl bead slurry for 1 ml extract for the Pre –clearing step in Immunoprecipitation ...
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Fasting blood serum samples were also fractionated on QAE Sephadex A-25 (GE Healthcare Bio-Sciences AB, Sweden) strong anion exchange particles ...
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bioRxiv - Biochemistry 2020Quote: ... the filtered medium was loaded on a 5 mL HisTrap Excel column (GE Healthcare) equilibrated with buffer AG.A (500mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... and isolated using the Ficoll Paque Plus density gradient medium (GE Healthcare Life Sciences) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and 5 ml of RPMI medium (HyClone GE Healthcare Life Sciences) with 5% FBS was injected in the mice peritoneum and left for 1 min before recovery by aspiration with a syringe ...
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bioRxiv - Cancer Biology 2022Quote: ... MOC1 cells were cultured in HyClone™ Iscove’s Modified Dulbecco’s Medium (IMDM; GE Healthcare Life sciences ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow was overlayed on Ficoll-paque density gradient medium (GE healthcare, Chicago, IL), washed twice with phosphate buffered saline (PBS) ...
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bioRxiv - Microbiology 2021Quote: ... Duke-NUS) [64] were all maintained in Dulbecco’s modified Eagle’s medium (DMEM, GE Healthcare), with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture medium was MEM/F-12 mixture (HyClone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (HyClone ...
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bioRxiv - Cell Biology 2023Quote: ... were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (HyClone GE Healthcare, Little Chalfont, UK) supplemented with 4.5 g/L glucose ...
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bioRxiv - Neuroscience 2022Quote: ... in Hanks’ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with 20 mg/mL DNase (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... and THP-1 g2c2 cells were cultured in Iscove’s modified Dulbecco’s medium (GE Healthcare) supplemented with 10% fetal bovine serum and penicillin/streptomycin (100 U/mL and 100μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat. # 17-0476-01, GE Healthcare). Second ...
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bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.