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Citations for GE Life Sciences :
401 - 450 of 1888 citations for Calponin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed 3 times for 5 min in TBST before incubation for 1 h at room temperature with secondary antibodies: donkey anti-mouse (1:5000; GE Healthcare, NA931-1ML), or donkey anti-rabbit (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2021Quote: ... Whole blood was diluted 1:1 with balanced salt solution at RT and layered onto Ficoll Paque PLUS (GE Healthcare, #GE17-1440-02) followed by centrifugation at 400 g for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... were freshly isolated from blood collected in EDTA-coated vials by layering diluted blood (1:1 in PBS) on top of an equal volume of Ficoll (GE Healthcare Life Sciences), followed by centrifugation and isolation of the buffy coat.
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bioRxiv - Microbiology 2022Quote: ... Protein samples were solubilized in DHHC Buffer C (1% SDS, 1 mM EDTA, 100 mM HEPES, pH 7.5) and were captured on thiopropyl sepharose 6B (GE Healthcare, 17-0402-01) in the presence of 2 M hydroxylamine or 2 M NaCl (negative control ...
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bioRxiv - Biochemistry 2020Quote: The human Cdc42 1-179aa Q61L (referred to as Cdc42)-encoding DNA was cloned into a modified pGEX-4T-1 vector (GE Healthcare, Chicago, Illinois) as previously described (Barthelmes et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were desalted using 500 µg of a 1:1 mixture of hydrophobic and hydrophilic Sera-Mag Carboxylate-modified Speed Beads (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with TBS + 0.1% Tween and incubated with goat anti-rabbit (1:2000, #NA934V) or anti-mouse (1:2000, #NA931V) secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Illinois) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... adjusted to 0.15 M NaCl and 1 % (v/v) Triton X-100 and were applied to a 1 mL Hi-Trap heparin column (GE Healthcare Life Sciences) equilibrated with a modified phosphate-buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... The final antisera were purified separately using His-DBN-1(1-132) bound to NHS-activated Sepharose High Performance (GE Healthcare Life Science). Both purified antibodies were tested in western blot ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The supernatant was diluted 1:1 with 20 mM sodium phosphate pH 7.4 and then purified using HisTrap Excel columns (GE Life Sciences # 17-3712-05) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µL of a 1:1 mixture of hydrophilic and hydrophobic carboxylate coated paramagnetic beads (SeraMag Speed Beads, #44152105050250 and #24152105050250, GE Healthcare, Little Chalfont, UK) were added to 30 µg protein of each sample ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Cell Biology 2020Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL™ Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Neuroscience 2021Quote: ... IRDye® 680LT goat anti-rabbit IgG (H + L) (LiCor, cat. #926-6802, 1:10,000) and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (GE Healthcare, cat. #AS09603, 1:10,000).
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Physiology 2023Quote: ... with TBS-T and incubated (1-hour, room temperature) with HRP-linked anti-rabbit secondary antibody (1:10,000; GE Healthcare Bioscience, Baie d’Urfe, Canada). Chemiluminescence was assessed using the Luminata™ Crescendo Western HRP Substrate (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... using a 1 kDa MWCO Mini Dialysis Kit (GE Healthcare). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... or sheep anti-mouse-HRP (GE healthcare, NXA931, 1:5000). Chemiluminescence detection was performed using the ECL Prime Western Blotting kit (GE-healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT) using a PD10 desalting column (GE Healthcare). The GST tag was removed via addition of Prescission protease (GE 27084301 ...
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bioRxiv - Cell Biology 2020Quote: ... and sheep ECL anti-mouse (1:3000, GE Healthcare, NA931V).
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bioRxiv - Cell Biology 2020Quote: ... Goat anti-mouse-HRP (GE Life Sciences #NA931, 1:5000) Ten micrograms of each sample were electrophoresed through a 12-well ...
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bioRxiv - Microbiology 2021Quote: ... Purification was performed on 1 ml HisTrap HP (GE Healthcare) column using an FPLC system (ÄKTA P-920 pump and Superdex 75 10/300 GL column ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-mouse IgG HRP linked (GE Healthcare NXA931, 1:7’500).
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bioRxiv - Developmental Biology 2020Quote: ... or donkey anti-rabbit (1:5000; GE Healthcare, NA934-1ML) coupled to Horseradish Peroxidase ...
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bioRxiv - Cancer Biology 2022Quote: ... or mouse (1:20000 dilution, NA9310; GE Healthcare Life Science) were incubated at room temperature with 1%–5% skimmed milk for 1 h ...
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bioRxiv - Physiology 2019Quote: ... supplemented with 1 mM sodium pyruvate (GE Healthcare, Thermo-Fisher), 1% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... and horseradish peroxidase-conjugated secondary antibodies (1:10000, GE Healthcare). Protein bands were visualised using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermofisher) ...
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bioRxiv - Genetics 2020Quote: ... and a 1 ml HiTrap Q HP column (GE Healthcare), both equilibrated in buffer B (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-tagged TMIGD1 constructs in pGEX-4T-1 (GE Healthcare): GST-TMIGD1 (hTMIGD1 cytoplasmic tail (AA 242-262) ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded onto a 1 ml HiTrap column (GE Healthcare) equilibrated in buffer B (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2020Quote: ... and loaded onto a 1 mL NHStrap column (GE Healthcare) previously coupled to 10-20 mg rabbit IgG (Innovative Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... ECL-anti-mouse HRP IgG (GE Healthcare, NA9310V, 1:10000), anti-goat IgG HRP (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM ATP and 0.25 µM dCTP-Cy3 (GE healthcare). The mixtures were then applied to biochips functionalized with a series of plasmids containing specific lesions ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies: anti-rabbit (1:10,000, GE Healthcare, Cat. #NA934V); anti-mouse (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... The pGEX-4T-1 vector (GE Healthcare Life Sciences, USA) was expressed in competent E ...
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Microbiology 2022Quote: ... and Nickel-Sepharose columns (HisTrap HP 1 ml, GE Healthcare) and was continued by size-exclusion chromatography using an ÄKTA FPLC apparatus (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... donkey anti-rabbit IgG-HRP (1:5000, #NA9340, GE Healthcare). Detection ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Biophysics 2020Quote: ... A 1 ml slurry of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Penicillin/Streptomycin (#sv30010, HyClone, GE Healthcare, Chicago, IL). The mantle cell lymphoma cell line Maver-1 (#ACC 717 ...
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bioRxiv - Molecular Biology 2022Quote: ... Using a HiTrap Heparin HP column (1 ml, GE Healthcare), proteins were fractionated via an increased gradient of NaCl ...