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Citations for GE Life Sciences :
401 - 450 of 2444 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Supernatant was mixed with GFP nanobody-conjugated Sepharose 4 Fast Flow resin (GE Healthcare). Resin was washed with buffer containing 0.06% digitonin ...
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bioRxiv - Neuroscience 2024Quote: ... purified from cell culture supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare) and dialyzed against PBS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Immunology 2024Quote: ... total cell isolates were resuspended in 4 mL of 70% Percoll solution (GE Healthcare), overlaid with 4 mL of 37% Percoll ...
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Genetics 2022Quote: ... 5% glycerol pH 7.5 (TNG buffer) and incubated for 30 min with 5 mL Ni-nitrilotriacetic acid (Ni-NTA) Fast Flow resin (GE Healthcare, Uppsala, Sweden) in a gravity-fed column to capture the fusion protein via its affinity to Ni-NTA ...
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Probe hybridization and horseradish peroxidase-mediated signal detection were performed using the ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, Chicago, IL), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Membranes were washed 3 times in TBST and incubated with Amersham ECL PLUS™ (GE Healthcare, RPN2132) prior to exposure to Super RX film (Fujifilm ...
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bioRxiv - Immunology 2021Quote: ... 2-D and 3-D images were obtained and analyzed using the software program MicroView (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Neuroscience 2020Quote: ... Binding was detected with an anti-mouse Cy-3 and anti-rabbit Cy-5 antibodies (GE Healthcare). Blots were scanned using a Typhoon 5 (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: The study was performed using a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Immunology 2020Quote: ... Post-nuclear supernatants were incubated with 3 μg Protein A Sepharose CL-4B (PAS; Ge HealthCare, #GEH17078001) for 1 hour and then with 3 μg PAS plus primary antibody (0.4 ug anti-GFP mAb ...
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bioRxiv - Neuroscience 2024Quote: The experiments were performed in a 3-Tesla scanner (HDx Signa platform, GE healthcare, Milwaukee, WI, USA) with an 8-channel head coil ...
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bioRxiv - Neuroscience 2023Quote: MRI scans were performed using a GE 3 Tesla MR750 Discovery scanner (GE Healthcare, Milwaukee WI, USA). The anatomical scan acquired a T1-weighted image with the MPRAGE sequence of TR/TE = 5/2 ms ...
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bioRxiv - Neuroscience 2023Quote: ... (3) Size exclusion chromatography (SEC): EVs were isolated from ISF using Sephadex-G25 (GE Healthcare; Uppsala, Sweden) following the vendor’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA of GmPHR1 was cloned into the prokaryotic expression vector pGEX-6p-3(GE Healthcare, USA) cleaved with BamHI ...
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bioRxiv - Neuroscience 2024Quote: ... The sample was then injected into 3 sequential HiTrap SP HP 5ml (15ml total) columns (GE healthcare). For cation exchange ...
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bioRxiv - Plant Biology 2024Quote: ... nuclei extracts from 3-week-old seedlings were transferred to a 0.2 μm nitrocellulose membrane (GE Healthcare) after separated by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was loaded on 3 × 5 mL HiTrap™ DEAE Sepharose FF (GE Healthcare, Munich, Germany) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered sample was incubated with 3 mL of CNBr activated Sepharose Fast Flow resin (GE Healthcare) beads coupled to GFP nanobody (42 ...
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bioRxiv - Developmental Biology 2020Quote: ... The fusion proteins were affinity-purified on Glutathione Sepharose® 4 Fast Flow (GE Healthcare), and then bound to POROS GoPure XS (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were precleared with Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 30 min at 4 °C under rotation ...
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bioRxiv - Plant Biology 2021Quote: ... coli strain BL21 (DE3) and purified with Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) by following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Synaptosomes were purified from 4-5 cerebella on discontinuous Percoll gradients (GE Healthcare, Uppsala, Sweden). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... and a multi-frequency linear-array transducer (L4-12T, 4–12 MHz, GE Healthcare, USA) was utilized to capture a VL image the transverse plane at approximately 50% the distance between the mid-inguinal crease and the proximal patella ...
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bioRxiv - Biochemistry 2022Quote: ITC measurements were performed at 4 °C using a MicroCal auto-iTC200 calorimeter (GE Healthcare). Samples were extensively dialyzed against a buffer containing 500 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... A second incubation overnight at 4°C with Protein G-magnetic Sepharose beads (GE Healthcare) was used to pull down protein:DNA complexes prior to washing in low salt (120mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-activated Sepharose 4 Fast Flow and Q-Sepharose Fast Flow was from GE Healthcare Life Sciences ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Protein A Sepharose CL-4B and Glutathione Sepharose 4 Fast Flow were from GE Healthcare Life Sciences (Chicago ...
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bioRxiv - Biochemistry 2022Quote: ... Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column, GE Healthcare) and SEC (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl and 4 mM β-mercaptoethanol using an Akta FPLC system (GE Healthcare). Pure His-tev-ecMSG or tag-cleaved ecMSG was concentrated to 40-80 mg/ml ...
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bioRxiv - Biophysics 2022Quote: SEC and DMC columns were prepared as described previously.4 Sepharose CL-4B (GE Healthcare) and Fractogel EMD SO3- (M ...
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bioRxiv - Molecular Biology 2023Quote: DNA was extracted from leaves of 4 week-old plants by Nucleon Phytopure (GE Healthcare) and total RNA was extracted from 3 week-old plants by RNeasy (QIAGEN ...
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bioRxiv - Biochemistry 2023Quote: ... incubated for 40 min at 4°C with nickel phosphate loaded Sepharose beads (GE Healthcare) to separate the His tag ...
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bioRxiv - Genomics 2023Quote: ... each AP2 domain was previously cloned into the pGEX-4-T1 vector (GE Life Sciences) and transformed into BL21- CodonPlus(DE3)-RIL E ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified from clarified supernatants using Protein G Sepharose 4 Fast Flow (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... This was incubated at 4°C overnight with 2 units of PreScission protease (GE Healthcare) in cleavage buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Cell Biology 2024Quote: ... equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Immunology 2024Quote: ... for 30 min at 4°C and with Streptavidin Sepharose High Performance beads (GE Healthcare) for 2h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).