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Citations for GE Life Sciences :
4351 - 4400 of 5529 citations for Mouse Anti Hantavirus Nucleocapsid Protein Antibody 4958 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... after primary and secondary antibody incubations and detected with ECL detection reagent (GE healthcare).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HPR)-conjugated secondary antibody (GE Healthcare, USA) diluted 1 in 2500 in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: The SOD specific antibodies were coupled to CNBr-activated Sepharose Fast Flow (GE Healthcare) following the manufacturer’s protocol at a concentration of 5 mg/mL wet gel ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing GFP and OFP fusion proteins were pulled down with GST-tagged anti-GFP and anti-mCherry nanobodies pre-bound to glutathione– Sepharose-4B beads (GE Healthcare #17075601), respectively ...
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bioRxiv - Immunology 2022Quote: ... HLA class-I and -II-associated peptides were isolated using the pan-HLA class I-specific mAb W6/32 and the pan-HLA class II-specific mAb TÜ39 (both in house mouse monoclonal) covalently linked to CNBr-activated Sepharose (GE Healthcare). HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid ...
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bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The sequence encoding mouse LRRC23 (residue 171-340 aa, CCDS20528.1) was cloned and inserted into pGEX6p-1 expression vector (GE healthcare), followed by transformation into E ...
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bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
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bioRxiv - Molecular Biology 2023Quote: Images of mouse spermatocyte spreads testicular cell squashes were acquired using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision) with a 60ξ NA 1.4 oil immersion objective ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-linked donkey anti-rabbit (NA934 GE Healthcare LifeSciences) were used as secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... HRP conjugated donkey anti-rabbit IgG (GE Healthcare, NA934V), 1:10000 ...
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bioRxiv - Neuroscience 2019Quote: ... or anti-rabbit HRP-conjugated (1:20,000; GE Healthcare) in blocking solution for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-rabbit (GE Healthcare Life Sciences, NA934V/AG) antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-rabbit conjugated HRP (1:500; GE Healthcare) as secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated with goat anti-GST (GE Healthcare) for an hour and GST binding was detected kinetically using an alkaline-phosphatase-linked rabbit anti-goat IgG secondary antibody (diluted 1:2000 in 0.1% BSA/PBS) ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit IgG HRP (1:5000 GE Healthcare NA934V), anti-mouse IgG HRP (1:5000 Abcam ab97046).
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bioRxiv - Plant Biology 2022Quote: ... using anti-Rabbit HRP-conjugated (1:5000, GE Healthcare) as a secondary antibody ...
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bioRxiv - Immunology 2024Quote: ... or peroxidase-conjugated anti-rabbit IgG (#NA934; GE Healthcare), were added as the secondary antibodies to the buffer in which the membrane was soaked ...
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bioRxiv - Biochemistry 2023Quote: ... or donkey anti-rabbit (1:10,000; GE Healthcare #NA934V).
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bioRxiv - Cell Biology 2022Quote: ... or anti-rabbit (GE Healthcare # NA9340, dilution 1:10,000) origin and ECL substrate (Thermo Scientific # 32106) ...
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bioRxiv - Immunology 2023Quote: ... or anti-rabbit IgG (for IgT detection; GE Healthcare), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-rabbit IgG (Cell signaling, GE Healthcare) used in a dilution of 1:10000 and incubated at room temperature for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... and goat-anti-rabbit were obtained from GE HealthCare/Amersham ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...