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Citations for GE Life Sciences :
4301 - 4350 of 6604 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Secondary antibodies included HRP-conjugated sheep anti-mouse IgG (GE Healthcare, NA931V, lot 9682503), HRP-conjugated donkey anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected using HRP-conjugated secondary anti-mouse (GE Healthcare®, NA931V) and anti-rabbit (GE Healthcare® ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated anti-mouse and anti-rabbit secondary antibodies (Cell Signaling or GE Healthcare) were used at a 1:10,000 dilution ...
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bioRxiv - Genetics 2019Quote: ... Bound secondary antibodies were detected with an Amersham Imager 600 (GE Healthcare Life Sciences) using chemiluminescence.
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were rabbit IgG HRP conjugated (GE Healthcare life sciences, Malborough, MA) or mouse IgG HRP conjugated (GE Healthcare life sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... and detected through horseradish peroxidase conjugated α-rabbit antibodies (GE Healthcare Life Sciences, NA934V). Experiments were repeated independently three times ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then incubated overnight with anti-BrdU antibody (RPN202, GE Healthcare, Buckinghamshire, UK) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare). Human MSLN (Advance BioMatrix 5123-0.1mg ...
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bioRxiv - Plant Biology 2022Quote: ... antibodies at proper dilution and visualized with enhanced chemiluminescence (ECL) reagent (GE Healthcare, USA).
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with horseradish peroxidase-linked anti-rabbit IgG (GE Healthcare, UK), and ECL Prime Western Blotting Detection Kit (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody–antigen complexes were visualized using the ECL plus chemiluminescence detection system (GE Healthcare) using the Stella imaging system (Raytest ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with horseradish peroxidase (HPR)-conjugated secondary antibody (GE Healthcare, USA) diluted 1 in 2500 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were detected using ECL Western Blotting Detection Reagent (GE Healthcare: #95038-562). Immunoblot band densitometry and normalization to GAPDH expression was performed using NIH ImageJ software35.
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bioRxiv - Cancer Biology 2023Quote: ... after primary and secondary antibody incubations and detected with ECL detection reagent (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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bioRxiv - Biophysics 2024Quote: ... we used horseradish peroxidase conjugated secondary antibodies against mouse (GE Healthcare, Cat. No.: NA931V) or rat (Jackson ImmunoResearch ...
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bioRxiv - Biophysics 2024Quote: ... The labeled antibodies were then purified using a NAP-5 column (17085302, GE Healthcare) and determined by a UV-Vis spectrophotometer.
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bioRxiv - Neuroscience 2024Quote: The SOD specific antibodies were coupled to CNBr-activated Sepharose Fast Flow (GE Healthcare) following the manufacturer’s protocol at a concentration of 5 mg/mL wet gel ...
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bioRxiv - Neuroscience 2024Quote: ... and sheep anti-Mouse IgG - Horseradish Peroxidase antibody (GE Healthcare, Cat # NA931; RRID: AB_772210).
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2020Quote: ... 9 mL of filtered immune serum were purified against the soluble part of the recombinant protein (10 mg) covalently coupled to 1mL HiTrap NHS-activated HP resin (GE Healthcare) according to the manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... 50 µL of 6 µM Cdt1 or Cdt1Uniform was prepared in Protein Buffer and then loaded and run on a Superose 5/150 GL sizing column (GE Healthcare) pre-equilibrated and run in Protein Buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was then purified by size exclusion chromatography (HiLoad 16/600 Superdex 75 pg column, GE Life Sciences cat#28989333). Purified protein was concentrated using Amicon Ulta 10k M.W ...
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biophysics 2021Quote: ... thrombin was removed and the proteins were concentrated by ion exchange chromatography on MonoQ or MonoS columns (Äkta purifying system, GE Healthcare). The Δ49-complex was assembled by first mixing syx-1 and the soluble syb 2 fragment (1:2 ...
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bioRxiv - Biophysics 2021Quote: ... the concentrated protein sample was loaded onto a Superdex 200 increase 10/300 GL size-exclusion column (GE Healthcare Life Sciences) and eluted in the gel-filtration (GF ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing the eluted and cleaved protein was concentrated down and applied onto a Superdex 75 column (16/60 prep grade, GE Healthcare) and eluted with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare). We labeled 50μM protein using 10μM SFP synthase (purified according to published protocols (53)) ...
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bioRxiv - Biophysics 2019Quote: ... The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Cell Biology 2020Quote: ... Ni-NTA–eluted SEC13/31A protein was further purified using an anion exchange column (MonoQ) on an AKTA FPLC system (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: GST-tagged proteins (Retromer, SNX3, WASHC2C-5 and WASHC2C-21) were purified by incubating clarified lysate with glutathione (GSH) Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cell Biology 2020Quote: ... 250 μg of MCF10A cell lysate and 5 μg of individual GST-tagged recombinant proteins were mixed and incubated with glutathione Sepharose 4b beads (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2019Quote: ... A final purification step was performed using size-exclusion chromatography by loading the proteins on a HiLoad 10/300 Superdex 200 column (GE Healthcare) using 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Size exclusion chromatography was performed to further purify proteins using AKTA FPLC using an S200 26/600 filtration column (GE Healthcare), following Khandokar et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE and examined by Western blotting using an Amersham imager 600 detection system (GE Healthcare). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample was centrifuged for 10 minutes at 12 000 g and the supernatant kept to pulldown biotinylated proteins by adding 200 µL of streptavidin-coupled magnetic beads slurry (Streptavidin Mag Sepharose, GE Healthcare) previously washed and equilibrated ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was kept to pulldown biotinylated proteins by the addition of 100 µL of streptavidin-coupled magnetic beads slurry (Streptavidin Mag Sepharose, GE Healthcare) previously washed and equilibrated ...
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bioRxiv - Molecular Biology 2021Quote: ... The secreted proteins were purified from the harvested media by two-step chromatography – an IMAC was performed on a Talon column (GE Healthcare), followed by SEC on a Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... The fractions containing the protein were pooled and applied onto a size-exclusion chromatography column (HiLoad 26/600 Superdex 200 pg, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequential affinity chromatography was performed on the combined fractions containing the desired asymmetric protein complexes from the size exclusion chromatography step by first using a Ni-sepharose HisTrap column (GE Healthcare) and then a StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the proteins were concentrated with Amicon Ultra 10K filter (viMillipore) and applied to HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions containing either AsCas12a or SpCas9 were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...