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Citations for GE Life Sciences :
4201 - 4250 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Cells for single cell RNA-seq were prepared by density centrifugation using Ficoll-Paque media (GE Healthcare, 17-5446-02) followed by magnetic-activated cell sorting (MACS ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21(DE3) cells and purified from the cell lysate using Glutathione-Sepharose 4B resin (Cytiva, Tokyo, Japan (GE Healthcare)) ...
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Individual activator and repressor transcription factors induce global changes in chromatin mobilitybioRxiv - Biophysics 2022Quote: ... Live cell imaging of CDX2-YPet and SIX6-YPet was performed using an IN Cell Analyzer 2200 apparatus (GE Healthcare) with a 20× magnification objective at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with glutathione beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspension was passed through a 40 μm plastic cell strainer and overlaid onto 10 mL Ficoll-Hypaque (GE Healthcare), followed by centrifugation (1000x g ...
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bioRxiv - Genetics 2021Quote: ... the percentage of eGFP-expressing cells/colonies were counted using an IN Cell 6000 confocal microscope (GE Healthcare, Marlborough, MA) and any well presenting less than 5% green fluorescent protein (GFP ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils (polymorphonuclear cells, PMNs) and peripheral blood mononuclear cells (PBMCs) were isolated by ficoll paque discontinuous gradient separation (GE Healthcare). PMNs were purified from the erythrocyte pellet by 5% dextran sedimentation ...
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bioRxiv - Cell Biology 2021Quote: Live cell imaging of cells depleted of TAAC3 and ROD1 was performed using a DeltaVisionElite system fluorescent microscope (GE Healthcare) equipped with a CoolSNAP HQ2 camera (Photometrics) ...
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bioRxiv - Immunology 2023Quote: ... Cell suspensions were filtered through a 40-μm cell strainer into RPMI with 10% FBS (GE Healthcare Life Sciences SH30071.03) and incubated for 5 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in standard procedures using 12% polyacrylamide gel (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare Life science, Germany) using TransBlot semi dry transfer cell (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 50 mM imidazole) before eluting the protein (HBS, 250 mM imidazole) following desalting using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium) to remove imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Neuroscience 2019Quote: ... Equivalent amounts of protein or equal volume from each sample were run on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (GE Healthcare, Piscataway, NJ, USA). Following blocking in 5% milk or bovine serum albumin the blots were incubated with primary antibodies and analyzed by HRP-conjugated secondary antibodies (Jackson Laboratories) ...
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bioRxiv - Pathology 2019Quote: ... Immunoreactive bands were detected by a FluorChem FC3 System (Protein-Simple, San Jose, CA, USA) after incubation of the membranes with ECL Selected Western Blotting Detection Reagent (GE Healthcare, Pittsburgh, PA, USA). Densitometric analyses of the immunoreactive bands were performed by the FluorChem FC3 Analysis Software.
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bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... using a HiTrap NHS-Activated HP affinity column coupled with aIL-4 monoclonal antibodies and an ÄKTA Fast Protein Liquid Chromatography (FPLC) instrument (GE Healthcare, Piscataway, NJ, USA). Immunizations ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The reacting agents were then separated from the now methylated proteins by size-exclusion chromatography using NapTM-5 columns according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK).
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were further resolved using size exclusion chromatography on gel filtration columns using the Superdex 200 (16/600) (GE Healthcare, GE28-9893-35) or Superdex 75 (16/600 ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were incubated in Bjerrum Schafer-Nielsen transfer buffer and proteins were transferred to a water-activated nitrocellulose membranes (Amersham Protran Premium 0.45 NC, GE Healthcare Life Sciences, Sunnyvale, USA) using semi-dry transfer (Trans-Blot® Turbo™ ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... 15-25 μg of total protein per sample were resolved on 8%-14% SDS-PAGE and transferred onto nitrocellulose membrane (Amersham Hybond, GE Healthcare Life Science, 10600002). Membranes were blocked in 5% milk in PBS followed by incubation with specific primary and secondary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was separated on the 10 % SDS PAGE and transferred on the polyvinylidene difluoride (PVDF) membranes (Hybond™-P; GE Healthcare, Piscataway, NJ, USA) using the wet transfer method (1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Following electrophoresis, protein was transferred onto a 0.45 μm nitrocellulose (Amersham Protran Premium, 10600096) or a PVDF membrane (GE Healthcare Amersham Hybond ECL, RPN68D). 0.4% PFA was used to fix the protein on the membrane ...
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bioRxiv - Biophysics 2021Quote: ... Amplified phage particles at 10-fold dilution were assayed against 20 nM biotinylated membrane proteins in nanodiscs using horse radish peroxidase (HRP)-conjugated anti-M13 monoclonal antibody (GE Healthcare, #27-9421-01). Assays were performed in library sorting buffer (200 mM NaCl ...
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bioRxiv - Pathology 2019Quote: ... Equal amounts of protein extracts (25 µg) were separated by SDS-PAGE before electrophoretic transfer onto a nitrocellulose membrane (GE Healthcare, Velizy-Villacoublay, France). Western-blot analysis was carried out using anti-LC3-II (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were transferred to nitrocellulose membranes and visualized by enhanced chemiluminescence using ECL Prime Western Blotting Detection Reagent (#RPN2232, GE Healthcare; Chicago, IL, USA) and imager ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Bio-Rad ChemiDoc XRS imaging system was used for visualizing the protein blot after incubations with ECL plus reagent (GE Healthcare, catalog no. RPN2132).
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Pathology 2020Quote: ... Equal volumes of plasma were pooled from three mice and lipoproteins were fractionated by fast protein liquid chromatography (ÄKTA pure FPLC system, GE Healthcare, Pittsburgh, PA, USA) and triglyceride and cholesterol in fractions quantified with reagent kits (Wako Diagnostics ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Molecular Biology 2021Quote: ... Then 6X-His-tagged wild type SufB precursor and mutant proteins were purified by Ni-NTA affinity column (Ni-NTA His trap, HP GE Healthcare Life Sciences-17524802) (23 ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Rv1339 protein was visualized with a FujiFilm LAS-3000 Image Reader and the Amersham™ ECL™ Western Blotting Analysis system (GE Healthcare, UK).
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were electrophoresed on an SDS polyacrylamide gel and then transferred to a nitrocellulose membrane (Amersham Protran Premium; GE Healthcare, Waukesha, WI, USA) using a semidry method ...
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a Superdex® 200 Increase 10/300 GL on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...