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Citations for GE Life Sciences :
4101 - 4150 of 4670 citations for Rat Androgen dependent TPF1 regulating protein ADTRP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and pre-cleared at 4°C for 1 h by incubating with 80 µl of protein A beads (#17127901, GE Healthcare, Freiburg, Germany), pre-equilibrated with ChIP dilution buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: Protein samples were subjected to 10% SDS-PAGE and the proteins were then electrophoretically transferred to Hybond-ECL nitrocellulose membranes (GE Healthcare, Amersham, UK). After blocking with 1% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2020Quote: ... These affinity-purified protein concentrates were then applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) packed with dextran covalently bound to highly cross-linked agarose ...
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bioRxiv - Neuroscience 2020Quote: Protein samples were separated by standard Laemli SDS-PAGE on 9% Tris-Glycine gels and transferred onto nitrocellulose membrane (GE Healthcare Bio-Sciences). PhosTag gels were prepared by adding 200 μM PhosTag™ (WAKO chemicals ...
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bioRxiv - Cell Biology 2022Quote: ... and expressed proteins were purified from the lysis supernatant by Nickel affinity and gel filtration (Superdex 75 26/60, GE Healthcare, Chicago, IL) chromatography ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were affinity-purified from filtered cultured supernatant on a High-Trap protein G column (GE Healthcare, Chicago, USA, Cat.Nr 17-0404-01).
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bioRxiv - Microbiology 2020Quote: ... The Vip3Aa200-end proteins were further purified by HiTrap Q HP ion-exchange chromatography and Superdex 200 gel filtration chromatography (GE Healthcare Life Sciences). Fractions containing the Vip3Aa200-end protein were concentrated to ∼ 7 mg/ml for crystallization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteoliposomes were formed by applying the lipid/protein containing sample (500 μl total volume) to an 18 ml detergent-removal column (Sephadex G-50 fine beads, GE Healthcare Life Sciences). Turbid fractions containing proteoliposomes were pooled ...
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bioRxiv - Molecular Biology 2021Quote: ... NET2 buffer was added to a final volume of 1 mL with 20 µL of Glutathione Sepharose 4B or SF3b155a-antibody conjugated with Protein G Sepharose (GE Healthcare Life Sciences) and incubated at 4°C for 3 h ...
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bioRxiv - Physiology 2021Quote: ... on an ÄKTA Pure Protein Purification System equipped with a Superdex 75 10/300 GL gel-filtration column and a 100 µL loop (GE Healthcare Life Sciences). Buffer was 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The six-His-tagged BclA3CTD protein was purified under native conditions using the His-Trap column as recommended by the manufacturer (GE Healthcare Life Science). The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: Association and dissociation reactions of GP96 to pneumococcal Ali proteins were analyzed using a BIAcore optical biosensor (BIAcore X-100 system, GE Healthcare Life Sciences), as previously described48 ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial protein PA1217 was fused to a GST-tag by cloning it into a pGEX-6P-1 vector (GE Healthcare, Chicago, IL) using the BamHI and EcoRI restriction sites (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 5 min in 0.05% PBST at RT and protein bands were subsequently visualized by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences) using EZ-ECL Chemiluminescence detection kit for HRP (Biological Industries ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified by affinity chromatography with nickel affinity columns (HisTrap 1 ml; Cytiva) using the Äkta pure system (GE Healthcare UK Ltd.). Elution was carried out at a flow rate of 1 ml/min with all solutions maintained at 4 °C and a linear imidazole concentration gradient from 30 mM to 530 mM.
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bioRxiv - Biophysics 2020Quote: ... The fractions with the protein were pooled and further purified by size exclusion chromatography using a 16/60 superdex 75pg column (GE Healthcare Life Sciences) equilibrated with a buffer containing 10 mM Tris pH 7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over Nap10 (GE Healthcare, 17-0854-01) and PD10 (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was further purified by size exclusion chromatography (SEC) using a HiPrep™ 26/60 Sephacryl™ S-200 HR (GE healthcare) column previously equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were further purified via size exclusion chromatography using a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare, Chicago, IL) using an AKTAExplorer 100 FPLC (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... affinity-purified proteins were further purified by size-exclusion chromatography (SEC) using a HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences) in 1x TBS (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a HiTrap MabSelect Sure column (GE Healthcare Life Sciences). Purified mAbs were buffer exchanged into PBS ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgG-YTE) were expressed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) and purified on HiTrap Protein-A column (GE healthcare, UK).
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bioRxiv - Immunology 2021Quote: ... Finkelman (University of Cincinnati, OH) (20) and purified from culture filtrates by standard protein G column chromatography (Amersham Biosciences/GE Healthcare, Piscataway, NJ). The hybridoma cells were grown in RPMI-1640 medium (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2021Quote: ... and 25µg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... dialysed into a Tris-saline buffer (Tris 10 mM, NaCl 150 mM, pH 7.4) then affinity purified on a protein A sepharose column (GE Healthcare, Chicago, IL, USA). Bound antibody was eluted with 0.1 M Glycine pH 2.4 and neutralised with 1 M Tris pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Bioengineering 2022Quote: ... The 100 mM and 250 mM fractions containing the eluted endolysins were dialyzed against PBS at 4 °C and they were further purified using gel filtration (fast protein liquid chromatography) on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The final step was LPS removal ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
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bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... High performance liquid chromatography (HPLC) was conducted using a HiTrap Q XL 1 mL Sepharose column on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). Proteins were eluted with starting buffer A followed by a gradually increasing concentration of buffer B (20 mM Bis-Tris ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a 5 mL HiTrap Chelating HP Sepharose column on the ÄKTA FPLC protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with 10 mL of starting buffer A (native buffer + 10 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... Samples then underwent size exclusion chromatography in an ÄKTA Pure fast protein liquid chromatography (FPLC) using HiLoad 16/600 Superdex 200 or Superdex 75 column (GE Healthcare Life Sciences) in GF-300 buffer (25 mM HEPES buffer at pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Biochemistry 2023Quote: ... The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science) anion-exchange column equilibrated with buffer A using the FPLC systems ...
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bioRxiv - Biophysics 2024Quote: ... Germany) and gel filtration fast protein liquid chromatography (FPLC) was performed on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The sample was dialyzed against phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Cellular lysates were cleared by centrifugation (14,000 rpm, 20 min) and then incubated (30 min) with Protein G-Sepharose beads (GE Healthcare, Mississauga, ON, Canada) followed by subsequent centrifugation (14,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and anti-mouse-ICAM-1 mAb (clone YN1/1.7.4) were produced by culturing hybridoma cells and purified using protein G sepharose chromatography (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Cancer Biology 2023Quote: ... 15 μg of the whole protein extracts was separated by SDS gel electrophoresis and transferred to a nitrocellulose membrane (Whatman, GE Healthcare, Freiburg, Germany). Rabbit polyclonal anti-SDHC (1:200 ...
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bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were purified from filtered culture supernatants by fast protein liquid chromatography (FPLC) on an ÄKTA Pure instrument using a 1 mL HiTrap MabSelect PrismA column (Cytiva, formerly GE Healthcare Life Sciences). Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma) ...
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bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...