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Citations for GE Life Sciences :
4101 - 4150 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (AmershamTM Protran® 0.45 µm, GE Healthcare Chicago, IL, USA). Membranes were blocked for 1 hour with 5% BSA in TTBS (15 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The TEV-cleaved protein was recovered by second immobilised metal affinity chromatography (IMAC) and further purified by SEC (S200 16/60 column, GE Healthcare) in SEC buffer under isocratic flow ...
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bioRxiv - Immunology 2024Quote: Tested antibody (5 μg/mL for NTD and 1 μg/mL for spike) was captured on protein A sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified SARS-CoV-2 mutant spike or NTDs ...
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bioRxiv - Bioengineering 2024Quote: ... The obtained filtrate was then applied to a Ni-NTA column using a fast protein liquid chromatography (FPLC) system from GE Healthcare. The column was equilibrated with potassium phosphate buffer A (100 mM ...
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bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
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bioRxiv - Developmental Biology 2024Quote: ... The GST-fused Ccrk protein was expressed in Escherichia coli strain BL21 (DE3) and purified using glutathione Sepharose 4 B (GE Healthcare). An antibody against Ccrk was obtained by immunizing guinea pigs with purified GST-fused Ccrk.
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bioRxiv - Synthetic Biology 2024Quote: ... and those found to contain the purified protein were pooled and concentrated using a 10 kDa molecular weight cut-off Vivaspin spin column centrifugal concentrator (GE Healthcare) at 3894 x g and 4°C ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... Abcam)] overnight in rotating conditions followed by incubation with 50 µl of 50% Protein A Sepharose-beads (GE Healthcare,17-0780-01) for 2 hrs at 4°C in rotating condition ...
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bioRxiv - Biochemistry 2024Quote: ... for 18 h at 18°C and N-terminal His6-tag fusion proteins purified by step-wise affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM tris-HCl (pH 7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Microbiology 2024Quote: ... The protein was further purified using size exclusion chromatography with a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare) into a final buffer of 20 mM HEPES pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein detection and quantification were performed using the SYBR-green fluorescence detection mode of the LAS-4000 image analyzer (GE Healthcare) and Image Quant TL software (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham, Piscataway, NJ, USA). After stripping ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Plant Biology 2024Quote: ... His6-tagged proteins (His6-HsfA2/S-HsfA2/S-HsfA4c/S-HsfB1/S-HsfB2a/DBD/tDBD) and His6 mock proteins were purified using glutathione Sepharose 4B beads (GE Healthcare) and nickel-nitrotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2024Quote: ... Full-length untagged cGAS protein was then ultimately purified using size-exclusion chromatography via a 16/600 Superdex S75 column (GE Healthcare). Purified cGAS was concentrated to ∼10 mg/ml in storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Ficoll Paque Plus used for isolating peripheral blood mononuclear cells (PBMC) cells was purchased from GE Healthcare. Staining antibodies ...
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bioRxiv - Genetics 2020Quote: ... with ∼5.0×104 cells per well and images were captured by IN Cell Analyzer 2200 (GE Healthcare) using the 60× objective lens.
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged for 72h in an IN CELL Analyzer 2000 microscope (GE Healthcare, Chicago, IL, USA) equipped with temperature and CO2 controller ...
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bioRxiv - Neuroscience 2022Quote: ... and then the live cells were imaged using an INCell Analyzer 6000 Cell Imaging System (GE Healthcare) and custom algorithms were used to detect fluorescent YFP-positive puncta (aggregates).
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare, Pittsburgh, PA) using AKTA pure FPLC system ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein separation was carried out with different ion exchange columns: A cation exchange column (HiScreen Capto SP ImpRes, GE Healthcare, München, Germany), an anion exchange column (HiTrap Q HP 1 ml ...
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bioRxiv - Biophysics 2019Quote: ... The protein bands were subsequently imaged using an Amersham Imager 600 instrument and its accompanying software (v0.9.8) (GE Healthcare, Chicago, IL, USA) with a 1 s exposure time ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... Cleaved protein was separated from TEV and the His-GST tag with a Ni-NTA column and proteins were further purified by size-exclusion chromatography on Superdex 75 16/600 (GE Life Sciences) in NMR buffer (10 mM MES ...
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bioRxiv - Neuroscience 2021Quote: ... Co-IPs done to ckeck for homodimers were done the same way except that agarose beads (Protein G Sepharose™ 4 Fast Flow by GE Healthcare) were used that were incubated with mouse anti-myc antibody (9B11 ...
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bioRxiv - Microbiology 2019Quote: ... Affinity-purified proteins were polished by size-exclusion chromatography (SEC) on Superdex 75 or Superdex 200 columns (GE Healthcare, Chicago, IL, USA) equilibrated with buffer B2 (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Co-immunoprecipitations of Rps3p with Bfr1p-3HA were performed with similar conditions to Bfr1p-yeGFP but immunoprecipitated with Protein G Sepharose® 4 Fast Flow beads (GE Healthcare). Prior to the immunoprecipitations ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences) equilibrated in Buffer C (25 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Biophysics 2019Quote: GST-fusion proteins (UBAN domains of OPTN, ABIN, NEMO and Ub) were cloned into pGEX-4T-1 (GE Life Sciences, Freiburg, Germany) and site-directed mutagenesis was performed by PCR to introduce desired amino acid substitutions.
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bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Yield can be improved by taking pooled TRAPP complex before SEC and manually running protein through a 1 mL HisTrap™ FF column (GE Healthcare) that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... TEV protease-digested proteins were passed over Ni-NTA agarose to remove uncleaved proteins and then further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed once with PBS containing 0.05% Tween-20 (PBST) and then incubated with Protein A/G Sera-Mag SpeedBeads (GE Life Sciences, 17152104010350) for 1 hour at 4°C with orbital shaking ...
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bioRxiv - Biochemistry 2021Quote: Samples were dialysed overnight in the corresponding buffers and different concentrations of protein were loaded onto a size-exclusion Superdex200 Increase 3.2/300 column (GE Healthcare Life Science) at a flow rate of 50 μL/min ...
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bioRxiv - Bioengineering 2021Quote: ... The eluted protein was then buffer exchanged to 10 mM HEPES at pH 7.4 by PD-10 columns (GE Healthcare Life Sciences) following the manufacturer’s instructions.