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Citations for GE Life Sciences :
4051 - 4100 of 6253 citations for Cow Rho Associated Protein Kinase 1 ROCK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein fraction obtained was further subjected to Hi-Load Superdex 200 pg 16/600 size-exclusion chromatography column (GE Healthcare/Cytiva) equilibrated with buffer B (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested by centrifugation 7 days after transfection and incubated with Protein G-coupled beads overnight at 4°C (GE Life Sciences). Beads were loaded onto chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the desired protein were pooled together and concentrated to 2ml using a 10 kDa cutoff Vivaspin20 concentrator (GE Healthcare Biosciences). Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: ... PD-10 desalting columns (Sephadex G-25 resin), and size exclusion chromatography column (HiLoad® Superdex, 75 pg) used for protein purification were obtained from GE Healthcare. Amorphous ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions with low protein background were pooled and subjected to size-exclusion chromatography on a Superdex 75 pg column (GE Healthcare, USA) in the storage buffer (DesAOMT ...
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bioRxiv - Microbiology 2023Quote: ... The first-generation immunoadhesins do not carry the Twin-Strep-tag and were purified using Protein A Mag Sepharose™ Xtra beads (50 µL beads/mL of supernatant; #28-9670-62, GE Healthcare) using binding buffer pH 7 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Genetics 2023Quote: ... Peaks containing the ThsA protein were injected to a size-exclusion chromatography (SEC) column (Superdex 200_16/60, GE Healthcare, 28-9893-35) equilibrated with SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: The concentrated affinity-purified protein sample underwent further purification by fast performance liquid chromatography (FPLC) using an AKTA purifier UPC10 system (GE Life Sciences) and a Hiprep 16-60 Sephacryl S300HR size exclusion chromatography column at a flow rate of 1 ml min-1 (running buffer was the same as the binding buffer but without the imidazole) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8x-His-SUMO tag was removed by Ni-NTA agarose beads and the proteins were further purified by size exclusion chromatography using Superdex 75 16/600 (GE Life Sciences) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using, ECL prime (Amersham, Uppsala, Sweden) and detected using ImageQuant™ LAS 4000(GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Microbiology 2023Quote: ... The amount of protein present in immunoblot bands was quantified using the ImageQuant LAS 4000 system with ImageQuant TL7.0 analysis software (GE Healthcare Life Sciences) or ChemiDoc MP (Bio- Rad ...
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity-purified protein was further purified (>95%) using Superdex 75 prep-grade matrix in a 16/70 C column (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2023Quote: The supernatant containing the target protein was then loaded on a 5 mL Ni-NTA column followed by a size exclusion column (Superdex 75, GE Healthcare, USA). YsiA is then eluted stepwise with 30 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Microbiology 2023Quote: The His6-tagged proteins were purified by affinity chromatography using HisTrap™ HP Ni-NTA columns and the ÄKTA purifier liquid chromatography system (GE Healthcare) according to the instructions of the manufacturers ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant LMW-SLP proteins from the soluble fraction were purified by affinity chromatography on Ni-agarose columns using an AKTA prime (GE Healthcare, #11001313). Proteins were dialyzed against 10 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL (GE healthcare, GE28-9909-44) in SEC buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Transfected cells were harvested 72 hours post-transfection and the 6 × His-tagged protein in the supernatant was purified using Ni-NTA (GE Healthcare, USA) affinity chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immuno-reactive protein bands were detected by the ECL system and visualized by imaging analyzer (ImageQuant LAS 500, GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... The purification of Ubi-SSFc and His6-SSFc was performed using HisTrap resin and AKTA protein purification system (Ge Healthcare Life Sciences). Firstly ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS– PAGE) and proteins were transferred into nitrocellulose membranes (Amersham Protran Premium 0.45μm nitrocellulose blotting membranes, GE Healthcare Life Sciences, USA). For immunostaining ...
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bioRxiv - Cell Biology 2024Quote: ... the protein signal was developed by Super Signal West Femto (Thermo 34095) and visualized with Amercham Hyperfilm ECL system (GE Healthcare 28906839) film.
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bioRxiv - Cancer Biology 2022Quote: ... a 1:1 mix of hydrophobic/hydrophilic Sera-mag carboxylate-modified speed beads (GE Healthcare) was prepared at a final bead concentration of 10 μg μL-1 ...
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bioRxiv - Immunology 2019Quote: ... Detection of immuno-reactive products was performed using 1:1 ECL reagent (#RPN2235, GE Healthcare) and a C-DiGit® Blot scanner (Model 3600 ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... A 1:1 stoichiometry was confirmed using SEC-MALS (S200, 10/300 GL GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Cell Biology 2024Quote: Cells were grown in 1:1 DMEM high glucose: Ham’s F-12 (Hyclone, GE Healthcare) supplemented with 5% fetal bovine serum (Hyclone) ...
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bioRxiv - Neuroscience 2021Quote: ... with precursor and reagent kits in single-use cassettes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... were precipitated using the 2D Cleanup kit (GE Healthcare, Chicago, IL), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... using ‘Illustra DNA extraction kit Phytopure’ (GE Healthcare, Little Chalfont, UK). To ensure that DNA originated from endophytic microorganisms ...
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bioRxiv - Biochemistry 2020Quote: ... Development utilized Enhanced Chemiluminescence Advance Kit components (GE Healthcare, PA, USA). Quantitation was carried out by densitometry with ImageJ (http://rsb.info.nih.gov/ij/index.html) ...
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bioRxiv - Plant Biology 2021Quote: ... detection was through the ECL Western Blotting Detection Kit (GE Healthcare) revealed via ChemiDocTouch from Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... membrane was developed with Amersham ECL prime kit (GE healthcare RPN2236) and imaged with Alpha Innotech imaging system.
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bioRxiv - Genomics 2020Quote: ... The immunocomplexes were detected using an ECL Prime kit (GE Healthcare) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using ECL Western Blotting Substrate kit (GE Healthcare). The intensity of the immunoreactive bands was quantified by densitometry using ImageJ software (National Institutes of Health).
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bioRxiv - Microbiology 2020Quote: ... Biotin CAPture Reagent provided in the Biotin CAPture Kit (GE Healthcare) was captured onto the sensor chip according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactive bands were detected by chemoluminescent detection (ECL kit, GE Healthcare) and images were acquired using the ImageQuant LAS 4000 (GE Healthcare Life Science) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... purified with IllustraTM DNA and Gel Band Purification Kit (GE Healthcare), and then cloned into pGEM®-T easy vector (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We used the 2-D Quant Kit (GE Healthcare, Uppsala, Sweden) to determine protein concentrations.
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... immuno-luminescence was detected using the ECL Prime kit (GE Healthcare) or ECL Clarity (BioRad ...
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bioRxiv - Plant Biology 2021Quote: ... The immunoreaction was developed using an ECL Prime Kit (GE Healthcare) and detected with Amersham Imager 680 blot and gel imager.