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Citations for GE Life Sciences :
351 - 400 of 2490 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... Slides were then washed twice with TBST (TBS with 0.05 % wt/v Tween 20) before incubating with the appropriate biotinylated secondary antibodies (GE Healthcare, 1:500) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated with horseradish peroxide (HRP)-conjugated) anti-rabbit IgG or anti-mouse IgG secondary antibodies (1:10,000, GE Healthcare Life Science) at room temperature and proteins were detected using Pierce ECL Western Blotting Substrate (34095 ...
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bioRxiv - Developmental Biology 2021Quote: ... The immunoblots were washed and incubated for 1 h at RT with HRP-linked secondary antibody donkey anti-rabbit IgG (GE Healthcare, NA934). The HRP-signal was detected using SuperSignalTM West Pico PLUS Chemiluminescent Substrate Kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used: donkey anti-rabbit IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK) and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and detected with a 1:10000 goat anti rabbit secondary antibody conjugated with HRP and imaged with ECL (GE healthcare NA931, NA934).
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bioRxiv - Microbiology 2020Quote: ... 10 μl of samples were loaded on 12% SDS-PAGE and transferred to Amersham Protran premium 0.2 μm nitrocellulose membranes (GE Healthcare Life Sciences) for 1 hour in an ice-cold transfer buffer (25mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Biophysics 2021Quote: ... The TLC plate was exposed to Fuji film BAS-MS 2025 imaging plate for 12 hr and analyzed using Typhoon FLA 9500 (GE Healthcare Ltd). The background obtained from reaction buffer (having no EccCb1 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences) and HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, IL). Membranes were rinsed in PBS + 0.05% Tween-20 before visualization.
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies include ECLTM anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (#NA934V, GE Healthcare) and ECLTM anti-mouse IgG Horseradish Peroxidase linked whole antibody (#NA931V ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody (ECL anti-mouse IgG horseradish peroxidase linked whole antibody) (GE Healthcare) was applied for 1 h in 1% skimmed milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were anti-Rabbit IgG – Horseradish Peroxidase antibody (GE Healthcare, Cat# NA9340 RRID: AB_772191), anti-HRP-conjugated antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... The bound protein was detected using rabbit anti-GH antibody at a final dilution of 1:40,000 (AFP5672099, NIDDK-NHPP) followed by horseradish peroxidase-conjugated antibody (anti-rabbit, IgG; GE Healthcare, Chicago, Illinois, USA) at a final dilution of 1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... NET2 buffer was added to a final volume of 1 mL with 20 µL of Glutathione Sepharose 4B or SF3b155a-antibody conjugated with Protein G Sepharose (GE Healthcare Life Sciences) and incubated at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed to remove unbound phages and then probed with anti-M13-HRP secondary antibody (1:5000, Cytiva [formerly GE Healthcare], Marlboro, MA) followed by TMB as noted above.
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V; anti-mouse, GE Healthcare, NA931V) for 2 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was then incubated in PBST containing a 1:2500 diluted secondary antibody (horseradish peroxidase-linked anti-mouse IgG from sheep; GE Healthcare, Cat. NA931V). After 3 rounds of 5-min wash with PBST ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... H2AX (total) (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Neuroscience 2021Quote: The SH-SY5Y cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and maintained in MEM/F-12 mixture containing 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2020Quote: The human neuroblastoma SH-SY5Y cells purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) were maintained in MEM/F-12 mixture containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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Stress response in Entamoeba histolytica is associated with robust processing of tRNA to tRNA-halvesbioRxiv - Microbiology 2021Quote: ... 20 μg RNA samples were separated on a denaturing 12% polyacrylamide gel and transferred to a membrane (Amersham™ Hybond™ -N+ Membrane, GE Healthcare). Probe DNA was 5′-end labeled by PNK reaction using γ-[32P]-ATP and hybridized with the membrane in perfectHyb buffer (Sigma ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10–12 % SDS-polyacrylamide gel electrophoresis gel (SDS-PAGE; Carl Roth) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Membranes were blocked for 1 h with 5 % non-fat milk in PBS-T (PBS with 0.1 % Tween-20 ...
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bioRxiv - Pathology 2021Quote: ... Excess reagents were removed by repeated diafiltration into fresh PBS buffer (140 mM sodium chloride and 12 mM sodium phosphates at pH 7.4) using VivaSpin sample concentrators (GE Healthcare, 10,000 Da MWCO), and the volume was corrected to 200 μL ...
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bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
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bioRxiv - Cell Biology 2022Quote: ... For immunoblotting the platelet lysates were separated by a SDS-polyacrylamide gel electrophoresis (12 % SDS-polyacrylamide gel) and electro-transferred onto a nitrocellulose blotting membrane (GE Healthcare Life sciences). After blotting ...
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bioRxiv - Neuroscience 2024Quote: ... 25 μg of protein was separated on a 12% SDS-PAGE gel and transferred to a Hybond-LFP PVDF membrane (GE Healthcare, Illinois, USA). The membrane was blocked in 5% skimmed milk in 1X Tris-buffered saline (TBS ...
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bioRxiv - Physiology 2024Quote: ... and a multi-frequency linear-array probe (12 L-RS, 5 – 13 MHz, 38.4-mm field of view, GE Healthcare, Milwaukee, WI, USA) were used to obtain ultrasonography images of the dominant VL and RF (based on kicking preference ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (GE Healthcare; # NA934V) incubated in PBST for 1h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Peroxidase-labelled anti-mouse IgG antibody and anti-rabbit IgG antibody (GE Healthcare, Chicago, IL, USA) were used as secondary antibodies for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Il USA). Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (#NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse secondary antibody (#NXA931 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
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bioRxiv - Biochemistry 2024Quote: ... the anti-HA antibodies (isotype mIgG2a) were captured with anti-mouse Fc antibodies (Biacore, GE Healthcare) which were immobilized on a CM5 sensor chip ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-rabbit IgG-HRP antibody and anti-mouse IgG-HRP antibody were purchased from GE Healthcare Life Sciences.
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bioRxiv - Cell Biology 2020Quote: ... HRP-linked antibody (NA934; GE Healthcare) as secondary antibody.
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were diluted 1:10,000 in TBS + 0.1% Tween-20 and applied to the membrane for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... IgG1 or IgG2a antibodies (GE Healthcare). The plates were washed and developed with 50 μl of TMB substrate per well until blue color appeared ...