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Citations for GE Life Sciences :
351 - 400 of 921 citations for Recombinant Mouse Lgals1 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: The green state of PhoCl1 and PhoCl1 empty barrel recombinant proteins were further purified with size exclusion chromatography using SD200 column (GE Healthcare) and buffer exchanged to 20 mM Tris pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...
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bioRxiv - Immunology 2019Quote: ... donkey anti-mouse-HRP (GE Healthcare), donkey anti-rabbit-HRP (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-mouse-HRP (GE healthcare, NXA931) 1:5000 and HRP Protein A (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG-HRP (GE Healthcare).
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bioRxiv - Cancer Biology 2019Quote: ... or sheep anti-mouse (GE Healthcare) IgG ...
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bioRxiv - Cancer Biology 2019Quote: ... and goat-anti-mouse (GE Healthcare), were used at a concentration of 1:10,000.
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bioRxiv - Developmental Biology 2019Quote: ... HRP-linked anti-mouse (GE HealthCare),
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bioRxiv - Molecular Biology 2020Quote: ... ECLTM anti-mouse IgG (GE Healthcare), and ECLTM anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-coupled α-mouse (GE Healthcare) 1:10000 ...
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bioRxiv - Plant Biology 2022Quote: ... sheep anti-mouse (NA931, GE Healthcare) was used at a dilution of 1:50,000 ...
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bioRxiv - Systems Biology 2022Quote: ... or anti-mouse (GE Healthcare NXA9311ML) peroxidase-linked secondary antibody (1:6000 dilution) ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse-HRP (GE Healthcare, NA931V). To visualise HS neoepitope ...
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bioRxiv - Cancer Biology 2024Quote: ... and goat-anti-mouse (GE Healthcare) secondary antibodies were used at concentrations of 1:10,000.
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bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Mt RnhC was concentrated and further purified by size exclusion chromatography using a 16/600Superdex® 200 pg columns (GE Healthcare). Mt RnhC eluted in a single peak and the fractions were pooled ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Molecular Biology 2020Quote: ... or anti-mouse antibody (GE Healthcare, NA931), blots were visualized using SuperSignal™ West Femto ECL substrate (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-mouse IgG (GE Healthcare, NA9310). Membrane was soaked with an ImmunoStar Zeta reagent (FujiFilm Wako Pure Chemical) ...
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bioRxiv - Microbiology 2020Quote: ... with HRP-conjugated anti-mouse (GE Healthcare) or HRP-conjugated anti-rabbit (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... donkey-anti-mouse-HRP (NA931, GE Healthcare), donkey-anti-rabbit-HRP (NA931 ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse (1:25,000; GE healthcare) IgG secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (NXA931V; GE Healthcare 1:5,000) and anti-rabbit (NA9340 ...
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bioRxiv - Cancer Biology 2019Quote: ... or sheep anti-mouse IgG (GE Healthcare) conjugated with horseradish peroxidase ...
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bioRxiv - Molecular Biology 2019Quote: ... An anti-mouse antibody (GE Healthcare, BR100838) was covalently coupled to a CM5 sensor chip (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-mouse-HRP (GE healthcare, NA931-1ML), anti-rabbit-HRP (GE healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-mouse IgG (GE Healthcare, NA931) were used as secondary antibodies at 1:1,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... HRP-coupled anti-mouse (NA9310V, GE Healthcare) or anti-rabbit (RPN4301 ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-linked Anti-mouse (GE Healthcare, NXA931), HRP-linked Anti-rat (Jackson Immunity ...
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bioRxiv - Microbiology 2024Quote: ... Secondary peroxidase labeled α-mouse (GE Healthcare), α-rabbit (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...