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Citations for GE Life Sciences :
351 - 400 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: His-tagged HexaPro was immobilized to a NiNTA sensorchip (GE Healthcare) to a level of ∼500 response units (RUs ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
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bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Neuroscience 2024Quote: ... DaT single-photon emission tomography was performed on a subset of patients and health controls using hybrid gamma camera Discovery 670 Pro system (GE Healthcare) with 123I-N-omega-fluoropropyl-2beta-carbomethoxy-3beta-(4-iodophenyl)nortropane (123I-FP-CIT ...
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bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The 6xHis-Lipo tag was cleaved off using Thrombin (GE Healthcare) and the protein was separated from the cleaved tag using the same column and buffers as described above ...
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bioRxiv - Plant Biology 2021Quote: ... tags could not be completely removed by PreScission protease (GE Healthcare). Arabidopsis Nudt7 (residues 1-322) ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Biochemistry 2024Quote: ... GST-tag was cleaved and removed by heparin-sepharose (GE Healthcare) purification and spDnmt2 was further purified by Superdex S75 size exclusion chromatography (Cytiva™) ...
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bioRxiv - Bioengineering 2021Quote: ... followed by purification on a His-Trap HP affinity column (GE Healthcare) using a gradient from 10 to 250 mM imidazole over 30 min in Tris buffered saline pH 7.5 in the presence of 1 mM betamercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... applied to Ni2+ ion affinity chromatography His Trap FF column (GE Healthcare), and purified using an Äkta pure chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then run through an equilibrated His-trap (GE healthcare) in an AKTA start fast protein liquid chromatography system ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a 5mL His-trap column (GE Healthcare) for protein purification and eluted with a linear imidazole (20 mM-200 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
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bioRxiv - Biochemistry 2020Quote: ... the cleaved MBP tag was removed with an MBPTrap column (GE Healthcare), and the protein was bound to a HiTrap heparin HP column (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cell Biology 2024Quote: Human full-length wild-type GCase protein (Cerezyme, Genzyme, Naarden, NL) was immobilized on an SPR CM5 sensor (GE Healthcare, #29149603) using standard amino coupling with a high protein concentration of 100 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: Human full-length wild-type GCase protein (Cerezyme, Genzyme, Naarden, NL) was immobilized on the SPR CM5 sensor (GE Healthcare, #29149603,) by standard amino coupling using relatively high protein concentration of 100 µg/mL ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was collected for purification using His Trap Excel column (GE Healthcare). The proteins were labeled with an AlexaFluor dye through the ybbR-tag and gel-filtered via a Superdex 75 10/300 GL column ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Neuroscience 2020Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on a Ni-NTA His-trap column (GE healthcare), washed with 10 column volume of buffer A containing 30 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were passed over a 1 ml His-Trap HF column (GE Healthcare). The bound protein was washed with 15 ml high salt wash buffer (20 mM potassium HEPES ...
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bioRxiv - Biochemistry 2021Quote: IMAC purification was performed using a His-Trap HP 1 ml (GE Healthcare) on an ÄKTA START protein purification system (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... and applied to a 1-ml chelating Hi-Trap Sepharose column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...