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Citations for GE Life Sciences :
351 - 400 of 4314 citations for Recombinant Human IL6 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the his-tagged FABP1 was purified using HisTrap HP affinity column (GE Healthcare, Chicago, IL), the tag was cleaved by thrombin and the cleaved protein was purified and buffer exchanged by gel filtration into 10 mM potassium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... a non-targeting scramble siRNA (#D-001210-01-05, GE Healthcare Dharmacon) was used in the experiments shown in figure 3 and supplementary figure 6C while a pool of four scrambled siRNAs (#D-001206-13-05 ...
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bioRxiv - Molecular Biology 2019Quote: ... and non-saturating exposures acquired on an ImageQuant LAS4000 system (GE Healthcare). Densitometry analysis was carried out using ImageQuant TL software.
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bioRxiv - Cell Biology 2022Quote: ... Non-targeting siRNA ON-TARGETplus (D-001810-10-05) (Dharmacon, GE Healthcare) served as the siRNA control (“siRNA scramble”) ...
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bioRxiv - Molecular Biology 2023Quote: ... The non-incorporated isotope was removed using G-25 columns (GE Healthcare) and the eluted DNA was precipitated with 2 vol of EtOH ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies were added as follows: biotinylated anti-mouse 1:200 (GE Healthcare RPN 1001) followed by Tyramide Super Boost with Alexa Fluor 488 1:500 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Bound biotinylated peptide was detected by addition of streptavidin-alkaline phosphatase conjugate (GE Healthcare, France) and 4-methylumbelliferyl phosphate substrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated Wuhan RBD ligand was immobilised on a streptavidin-coated (SA) sensor chip (GE Healthcare) at 2453 RU ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged products were then purified using Glutathione Sepharose 4B according to manufacturer’s instruction (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... GST-tagged PfHDAC1 was purified from the soluble fraction with glutathione Sepharose 4B beads (GE Healthcare) using 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2021Quote: ... or GFP tagged INO1 were acquired on a DeltaVision Elite imaging system (GE Healthcare Life Sciences) with a 60x/1.42 NA oil ...
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Biochemistry 2022Quote: Purified GST-tagged DIDATRX and DIDATRX fragments were immobilized on Glutathione sepharose 4B beads (GE Healthcare) under saturating conditions in binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... targeting siRNA and non-targeting scramble control siRNA were purchased from GE Healthcare (mouse Dnmt1 siRNA-SMARTpool (L-056796-01) ...
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bioRxiv - Neuroscience 2022Quote: ... Bound antibodies were detected by applying biotinylated goat anti-rabbit IgG (GE Healthcare, Velizy Villacoublay, France) for 2h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The biotinylated GRP78 was captured onto a streptavidin-coated Sensor Chip SA (GE Healthcare, NJ, USA) for analyzing the binding of a synthetic GRP78-targeting peptide WDLAWMFRLPVG (GL Biochem Ltd ...
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bioRxiv - Developmental Biology 2020Quote: ... Biotinylated PCR products (20μL) were immobilized on Streptavidin-coated Sepharose beads (GE Healthcare, 17-5113-01). DNA strands were separated using the PyroMark Q24 Vacuum Workstation ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... L7TIR and MBP tagged L7TIR were subjected to Superose 6 10/300 gel filtration column (GE Healthcare). Fractions eluted at void volume were collected ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... the Fc tag was cleaved by O/N incubation with GST-tagged 3C PreScission Protease (GE Healthcare) in cleavage buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Microbiology 2019Quote: ... cAMP was quantified using a cAMP Direct Biotrak EIA (non-acetylation) kit (GE Healthcare). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Labelled oligonucleotides were separated from non-incorporated nucleotides via G50 MicroSpin columns (GE Healthcare). 1 μg of total RNA from the indicated gradient fractions were annealed to 0.3 pmol of radiolabelled specific primer in 30 mM Tris-Cl pH 7.5 and 2 mM KCl at 90°C for 2 min and then cooled down to room temperature for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... or the respective negative controls (non-targeting small interfering RNA, siCTR, GE Healthcare Dharmacon) using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific #13778-150 ...
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bioRxiv - Biochemistry 2022Quote: ... Approximately 300-400 resonance units (RU) of FPLC-purified biotinylated arrestin in HBS-P+ Buffer (GE Healthcare) were captured on an SA-chip (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Site-specifically biotinylated RBD was immobilized on streptavidin sensor chips (Series S sensor Chip SA, GE Healthcare) to a level of 200 resonance units (RU) ...
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bioRxiv - Immunology 2021Quote: ... Site-specifically biotinylated RBD was immobilized on streptavidin sensor chips (Series S sensor Chip SA, GE Healthcare) to a level of ~200 resonance units (RU) ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tagged phytochrome samples were first purified with NiNTA affinity purification using HisTrap™ columns (GE Healthcare), followed by size-exclusion chromatography (HiLoad™ 26/600 Superdex™ 200 pg ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and the His-tagged sec49K was purified with HisTrap excel Ni beads (GE Healthcare), eluted with 300 mM imidazole and dialysed against buffer containing 50% glycerol (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Hexahistidine-tagged AldA was further purified through a Superdex-200 26/60 size-exclusion column (SEC) (GE healthcare) in 25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The biotinylated PCR products were purified on a MiniQ 4.6/50 ion exchange column (GE Healthcare Life Sciences) and loaded into a HiTrap Streptavidin HP column (Amersham Biosciences) ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... Individual tRNA species were bound to biotinylated DNA probes anchored to high-capacity streptavidin agarose resin (GE Healthcare) in 30 mM Hepes-KOG ...
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bioRxiv - Biochemistry 2022Quote: ... The biotinylated Norrin samples were immobilized onto the surface of a CM5 sensor chip (GE Healthcare Life Sciences) on which approximately 8500 resonance units of streptavidin were coupled via primary amines ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...