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Citations for GE Life Sciences :
351 - 400 of 1885 citations for R 3 Amino 5 hexynoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA HisTrap columns (GE Healthcare, Chicago, IL, USA) were used ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... and loaded onto a HisTrap HP 5 ml column (GE Healthcare) at 4 °C using an ÄKTA Pure 25 M (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... with Nickel affinity chromatography (5 mL HisTrap HP column, GE Healthcare), anion ion exchange chromatography (5 mL HiTrap Q FF column ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity chromatography utilizing a HisTrap HP 5 mL column (GE Healthcare) was then employed to purify the protein in binding buffer consisting of 50 mM NaPO4 (pH 7.4) ...
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bioRxiv - Molecular Biology 2023Quote: ... MSN was buffer exchanged (NAP-5 column; GE Healthcare Life Sciences) and diluted to 20 µM in ITC buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded onto a 5 mL HiTrap SPHP column (GE Healthcare). A linear 5 CV gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was injected in S200 5/150 column (GE Healthcare) pre-equilibrated with KMNZ reconstruction buffer and the peak fraction was taken for structural analysis.
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bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL HisTrap FF column (GE Healthcare) at a rate of 2 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... 5 ml HisTrap FF crude columns were bought from GE Healthcare. NuPAGE® MES SDS Running Buffer (20X) ...
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bioRxiv - Biochemistry 2023Quote: ... RNGTT was further loaded onto Mono Q (5/50GL, GE Healthcare) while CMTR1 was further loaded onto a Heparin column (5/50GL ...
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bioRxiv - Microbiology 2023Quote: ... and applied to 5 ml HiTrap Q HP column (GE Healthcare) equilibrated with TGE buffer containing 50 mM NaCl and 1 mM β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... before loading onto a 5 ml HisTrap HP column (GE Healthcare). The protein was eluted in 300 mM imidazole ...
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bioRxiv - Synthetic Biology 2023Quote: ... using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) as previously described3 except that an optimized buffer gradient was employed consisting of a first wash step of 5 column volumes (CV ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was applied to HisTrap HP 5 ml column (GE Healthcare) pre-equilibrated in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Biochemistry 2024Quote: ... Excess TCEP was removed using a NAP-5 column (GE Healthcare) pre-equilibrated with cold-degassed PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... each layered on 5 mL of Ficoll-Paque Premium (GE Healthcare) in 15 mL sterile Falcon tubes ...
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bioRxiv - Biochemistry 2024Quote: ... and 5% glycerol using an AKTA Start FPLC system (GE Healthcare). The column was washed with 10 CV of the equilibration buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Excess dye was removed using NAP-5 columns (GE Life Sciences). Fractions containing ssDNA were identified using absorbance measurements at 260 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... and applied to 2 × 5-mL StrepTrap HP columns (GE Healthcare), connected in series ...
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bioRxiv - Biochemistry 2024Quote: ... for IgG expression and 5-ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting supernatant was incubated with 3 mL equilibrated Glutathione-Sepharose 4B beads (GE Healthcare) for 45 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM DTT and was loaded onto a MonoS 10/100 column (GE Healthcare). The protein was eluted with an increasing salt (KCl ...
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bioRxiv - Neuroscience 2022Quote: Images were acquired using a 3 tesla MRI scanner (Discovery MR750; GE Healthcare, Waukesha, Wisconsin) equipped with a 32-channel head coil ...
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bioRxiv - Molecular Biology 2024Quote: Raw 3-phase images generated by 3D-SIM were reconstructed using SoftWorX (Applied Precision, GE), and these reconstructed stacks were 3D rendered using the 3D script plugin 31 in FIJI ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplification product of WRKY45 was cloned into pGEX-6P-3 vector (GE Healthcare, USA) that was cleaved with BamHI ...
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bioRxiv - Neuroscience 2023Quote: Whole brain images were acquired with a 3-T MRI scanner (Discovery MR750; GE Healthcare) at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare, Velizy-Villacoublay, France) in cold blotting buffer (25mM Tris ...
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bioRxiv - Neuroscience 2024Quote: All MRI data were acquired on GE 3 T Signa Premier (GE Healthcare, Chicago, Illinois) with a 48-channel head coil ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Biophysics 2024Quote: ... and the (His)6-tagged protein was purified using affinity chromatography with Nickel nitrilotriacetic acid (Ni-NTA) according to the manufacturer’s recommendations (GE Healthcare, UK). SDS-PAGE analysis validated the purification of rESAT-6 ...
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bioRxiv - Biochemistry 2024Quote: WT ubiquitin was expressed in a tagless form and purified with the glacial acetic acid method followed by gravity cation exchange chromatography (GE Healthcare) and SEC as described previously.77
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bioRxiv - Neuroscience 2022Quote: ... For AKT1 detection, anti-AKT (pan) (11E7) (Cell Signalling Technology, #4685, 1:1000 in 5% BSA) and donkey-anti-rabbit IG (GE Healthcare, NA9340V, 1:3000 in 5% BSA) antibodies were used ...
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bioRxiv - Biochemistry 2020Quote: ... The 2.5 ml of R-β-actin sample was desalted to remove DTT leftovers by using PD-10 (Prepacked Disposable PD-10 Columns, GE Healthcare #17085101) column pre-equilibrated with G-buffer without DTT ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-Scp160 antibodies (Gift of R. Jansen, U. Tuebingen) was performed using the Amersham ECL Western Blotting Detection Kit (GE Healthcare Life Sciences). Quantification of protein bands was performed using ImageJ software.
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bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... a stabilized soluble version of the spike protein (based on GenPept:QHD43416 ORF amino acids 1–1207) was produced using an ExpiCHO Expression system (Thermoscientific, USA, Cat. No. A29133) following purification using HisTrap (GE Healthcare, U.K.) and Strep-TactinXT (IBA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was applied to a 5 ml StrepTactin column (GE Healthcare, Sweden) and eluted with 2.5 mM desthiobiotin in 25 mM HEPES pH 7.5 ...