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Citations for GE Life Sciences :
351 - 400 of 719 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... was expressed as fusion protein with an N-terminal glutathione-S-transferase (GST)-tag using the pGEX-6P-1 vector (GE Healthcare). A synthetic PNPLA1 gene ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with an Olympus UPIanApo 100× numerical aperture 1.35 oil objective with the complementing immersion oil (N=1.516, Applied Precision, Issaquah, WA), Standard DeltaVision filters FITC and Rhodamine ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... 45 μl was spotted to a double-membrane system on a dot-blot apparatus with an upper nitrocellulose membrane and a lower hybond-N+ membrane (GE Healthcare). After filtration under vacuum ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Biochemistry 2023Quote: The GSTs contained an N-terminal hexahistidine tag enabling their purification by immobilized metal affinity chromatography (IMAC) using a Ni-IMAC column (GE Healthcare). The supernatant fraction from the centrifugation was loaded onto the column equilibrated with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by removal of the N-terminal His6-Ztag by TEV protease cleavage and size exclusion chromatography using a Superdex 200 16/60 column (GE Healthcare) (Roostalu et al ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
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bioRxiv - Microbiology 2021Quote: ... dadantii OutB was cloned into the pGEX-6P-3 vector (GE Healthcare) encoding a cleavable N-terminal Glutathione S-transferase (GST ...
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bioRxiv - Cell Biology 2019Quote: ... 3 were acquired using DeltaVision Elite wide-field microscope system (Applied Precision). Images were acquired as z-stacks with a step size of 0.5 μm ...
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bioRxiv - Biochemistry 2022Quote: ... and 3 mM MgCl2] using a size exclusion column (NAP5; GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... using a multi-frequency linear array transducer (3-12 MHz, GE Healthcare). Participants were instructed to lay on an athletic training table in the supine position 5 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 3D-SIM was carried out using an OMX Version 3 (Applied Precision) microscope equipped with a UPlan APO 100× 1.4 NA oil-immersion objective (Olympus) ...
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bioRxiv - Cancer Biology 2024Quote: ... for 3-5 minutes and visualized using Amersham Imager 600 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... The elute from Q Sepharose was loaded on to 0.20 L source 15RPC (GE Healthcare, USA) column to separate non-glycosylated and glycosylated rhEPO ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Heeseon An) were maintained in McCoy’s 5A (modified) medium with L-Glutamine (#SH30200.01, GE Healthcare, USA) medium supplemented with a 10% final concentration of fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of DNA from each specimen were spotted on a piece of Nytran N nylon blotting membrane (GE Healthcare Life Sciences). The membrane was incubated with a primary antibody that recognized Pt-induced DNA adducts (ICR4 ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Molecular Biology 2020Quote: ... and 49A were generated by inserting the BamHI–EcoRI PCR fragments amplified from pEGFP plasmids into the BamHI–EcoRI sites of the N-terminal GST-fused protein expression vector pGEX-6P-2 (GE Healthcare, US). Recombinant proteins were obtained as described in Supporting Methods 1 ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were separated by electrophoresis using a 1% agarose gel and transferred to nylon membranes (Hybond N+®, GE Healthcare), where hybridization was carried out using an 812 bp fragment of Micropia from D ...
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bioRxiv - Plant Biology 2024Quote: ... Enrichment of N-terminal labeled peptides was carried out using 200 µL NHS-Sepharose slurry (17-0906-01, GE Healthcare, Solingen, Germany) per 100 µg protein ...
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bioRxiv - Microbiology 2019Quote: ... IEF was performed using 24 cm 3–7 NL Immobiline DryStrip (GE Healthcare) in an IPGphor unit (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... placed on Ficoll-Paque Plus (ratio 7:3) (GE Healthcare Life Sciences, 17144003), and centrifuged for 20 min at 1800 rpm at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Immunology 2022Quote: ... and 3 mL of 66% percoll was underlaid (GE healthcare 17–0891–01). Samples were spun for 20 min at 2000 rpm at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
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bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HUVEC were attached to dextran-coated Cytodex 3 beads (GE Healthcare Bio-Sciences) and embedded at 250 beads per well in a 24-well plate in a fibrin clot ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM NaN3) on a Superdex 200 Increase 5/150 column (GE Healthcare) equilibrated by the same buffer and run at a 0.45 ml/min flow rate ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...